PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 26636375-4 2016 ATM or p53 down regulation was shown to modulate differently the cellular fate of H(2)O(2)-treated hMESCs. Hydrogen Peroxide 82-90 tumor protein p53 Homo sapiens 7-10 26891889-5 2016 Sublytic concentrations of hydrogen peroxide (H2O2) plus NO donor S-nitroso-N-acetyl-D, L-penicillamine (SNAP) enabled to induce a toxic oxidative/nitrosative stress through activating both p38 MAPK and p53 cascades, and cause DNA damage and protein tyrosine nitration in primary neuronal cultures. Hydrogen Peroxide 27-44 tumor protein p53 Homo sapiens 203-206 26891889-5 2016 Sublytic concentrations of hydrogen peroxide (H2O2) plus NO donor S-nitroso-N-acetyl-D, L-penicillamine (SNAP) enabled to induce a toxic oxidative/nitrosative stress through activating both p38 MAPK and p53 cascades, and cause DNA damage and protein tyrosine nitration in primary neuronal cultures. Hydrogen Peroxide 46-50 tumor protein p53 Homo sapiens 203-206 26636375-5 2016 ATM inhibition allowed H(2)O(2)-stimulated hMESCs to escape the permanent cell cycle arrest due to loss of the functional ATM/p53/p21/Rb pathway, and induced bypass of mitosis and re-entry into S phase, resulting in tetraploid cells. Hydrogen Peroxide 23-31 tumor protein p53 Homo sapiens 126-129 26636375-6 2016 On the contrary, suppression of the p53 transcriptional activity caused a pronounced cell death of H(2)O(2)-treated hMESCs via autophagy induction. Hydrogen Peroxide 99-107 tumor protein p53 Homo sapiens 36-39 26535076-5 2015 H2O2-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 171-174 27375834-5 2016 H2O2 produces both nuclear and mitochondrial DNA lesions, increases the expression of cell adhesion molecules, and increases p53 activity and other transcription factors orchestrating cancer cell death. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 125-128 26041389-5 2015 The expressions of p53 and p21 were decreased in H2O2-treated RTEF-1 o/e human umbilical vein endothelial cells. Hydrogen Peroxide 49-53 tumor protein p53 Homo sapiens 19-22 26208454-3 2015 Exogenous H2O2 tended to promote SIRT1 expression and induce more autophagy through mTOR pathway in control ph ESCs, by contrast more apoptosis via activation of p53 and caspase-3 in SIRT1-knockdown ph ESCs. Hydrogen Peroxide 10-14 tumor protein p53 Homo sapiens 162-165 25789565-7 2015 Here, we show that p53 phosphorylation at Ser 33 contributes to H2O2-induced miR-200s transcription. Hydrogen Peroxide 64-68 tumor protein p53 Homo sapiens 19-22 26191131-10 2015 In addition, GQP significantly affected the p53-p21 and p16-pRb pathways in H2O2-treated WI-38 cells. Hydrogen Peroxide 76-80 tumor protein p53 Homo sapiens 44-47 25766317-6 2015 For the first time, we show that persistent exposure to low levels of the ROS, hydrogen peroxide, leads to the long-term expression of p21 in HNSCC cells with a partially functional TP53, resulting in senescence. Hydrogen Peroxide 79-96 tumor protein p53 Homo sapiens 182-186 25576341-9 2015 H2O2 upregulated p53 and miR-143, but downregulated ATG2B, Bcl-2, and LC3-I expression in U2OS cells (wild-type p53) but not in SAOS-2 (p53-null) cells. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 17-20 25576341-9 2015 H2O2 upregulated p53 and miR-143, but downregulated ATG2B, Bcl-2, and LC3-I expression in U2OS cells (wild-type p53) but not in SAOS-2 (p53-null) cells. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 112-115 25576341-9 2015 H2O2 upregulated p53 and miR-143, but downregulated ATG2B, Bcl-2, and LC3-I expression in U2OS cells (wild-type p53) but not in SAOS-2 (p53-null) cells. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 112-115 25818185-11 2015 Finally, pretreatment of cells with DMY prior to H2O2 exposure resulted in the inhibition of p53 activation, followed by the regulation of the expression of Bcl-2 and Bax, the release of cytochrome c, the cleavage (activation) of caspase-9 and caspase-3, and then the suppression of PARP cleavage in H2O2-induced HUVECs. Hydrogen Peroxide 49-53 tumor protein p53 Homo sapiens 93-96 25535325-4 2015 In the present study, we found that H2O2 induced apoptotic cell death in p53-positive human hepatocytes, but not in p53-negative human hepatocytes. Hydrogen Peroxide 36-40 tumor protein p53 Homo sapiens 73-76 25789565-8 2015 In addition, we show that p38alpha can directly phosphorylate p53 at serine 33 upon H2O2 exposure. Hydrogen Peroxide 84-88 tumor protein p53 Homo sapiens 62-65 27012093-1 2015 Human endometrium-derived mesenchymal stem cells (hMESC) under the sublethal oxidative stress induced by H2O2 activate both p53/p21/Rb and p38MAPK/MAPKAPK-2 pathways that are responsible for the induction of hMESC premature senescence (Borodkina et al., 2014). Hydrogen Peroxide 105-109 tumor protein p53 Homo sapiens 124-127 25412667-8 2014 Pretreatment with TJ-10 inhibited H2O2-induced up-regulation of p53 and enhanced AMPKalpha1 expression. Hydrogen Peroxide 34-38 tumor protein p53 Homo sapiens 64-67 24749765-8 2014 H2O2 increased the expression of BAX, BAD, p53 and caspase-3 in a time-dependent manner, those of which anthocyanin significantly decreased. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 43-46 24845634-10 2014 RESULTS: Pulsed H2O2 exposure triggered the acetylation of p53 at lysine 382 (K382) and subsequent increase in its target p21(Waf1/Cip1). Hydrogen Peroxide 16-20 tumor protein p53 Homo sapiens 59-62 25108166-4 2014 Our results revealed that H2O2 reduced viability of cells through up-regulation of p53 followed by increase in Bax/Bcl2 ratio. Hydrogen Peroxide 26-30 tumor protein p53 Homo sapiens 83-86 25431609-5 2014 In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Hydrogen Peroxide 13-17 tumor protein p53 Homo sapiens 211-214 24553354-0 2014 Hydrogen peroxide/ATR-Chk2 activation mediates p53 protein stabilization and anti-cancer activity of cheliensisin A in human cancer cells. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 47-50 24634836-1 2014 The regulatory mechanisms by which hydrogen peroxide (H2O2) modulates the activity of transcription factors in bacteria (OxyR and PerR), lower eukaryotes (Yap1, Maf1, Hsf1 and Msn2/4) and mammalian cells (AP-1, NRF2, CREB, HSF1, HIF-1, TP53, NF-kappaB, NOTCH, SP1 and SCREB-1) are reviewed. Hydrogen Peroxide 35-52 tumor protein p53 Homo sapiens 236-240 24634836-1 2014 The regulatory mechanisms by which hydrogen peroxide (H2O2) modulates the activity of transcription factors in bacteria (OxyR and PerR), lower eukaryotes (Yap1, Maf1, Hsf1 and Msn2/4) and mammalian cells (AP-1, NRF2, CREB, HSF1, HIF-1, TP53, NF-kappaB, NOTCH, SP1 and SCREB-1) are reviewed. Hydrogen Peroxide 54-58 tumor protein p53 Homo sapiens 236-240 25431609-5 2014 In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Hydrogen Peroxide 13-17 tumor protein p53 Homo sapiens 54-57 23875998-3 2013 In the present work, we show that p53-expressing cells are more susceptible to cell death after an apoptotic stimulus such as H2O2. Hydrogen Peroxide 126-130 tumor protein p53 Homo sapiens 34-37 25147862-4 2014 Further results indicated that Vit C caused the dysregulation of some stress responses factors (SIRT1, p53 and FOXO3) in ARPE-19 cells response to H2O2. Hydrogen Peroxide 147-151 tumor protein p53 Homo sapiens 103-106 24188375-8 2013 Hydrogen peroxide generation by oxidation of 2",7"-dichlorodihydrofluorescein diacetate to the fluorescent compound 2",7"-dichlorfluorescein assay, acridine orange/ethidium bromide staining, flow cytometry analysis of annexin-V/7-amino-actinomycin, mitochondrial membrane potential and immunocytochemistry detection of transcription factor p53, caspase-3 and apoptosis-inducing factor (AIF) were evaluated. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 340-343 23852422-0 2013 Cheliensisin A inhibits EGF-induced cell transformation with stabilization of p53 protein via a hydrogen peroxide/Chk1-dependent axis. Hydrogen Peroxide 96-113 tumor protein p53 Homo sapiens 78-81 23955846-5 2013 The results reveal that H2 O2 can induce apoptosis effectively by regulating the activity of apoptosis-related biomolecules, including pro-apoptotic factors p53 and Bax, and anti-apoptotic factor Bcl-2. Hydrogen Peroxide 24-29 tumor protein p53 Homo sapiens 157-160 24198727-4 2013 Both H2O2 treatment and AdRas12V infection induced senescence in VECs, as assessed by senescence-associated beta-Gal activity and the expression of proteins such as p53 and p21(CIP1). Hydrogen Peroxide 5-9 tumor protein p53 Homo sapiens 165-168 23852422-9 2013 Finally, increased hydrogen peroxide was found to mediate Chk1 phosphorylation at Ser345, p53 protein induction, cell apoptotic induction, and transformation inhibition following Chel A treatment. Hydrogen Peroxide 19-36 tumor protein p53 Homo sapiens 90-93 23417568-6 2013 We also observed that menadione induced JNK-dependent p53 expression and apoptotic death in SK-N-MC cells while H2O2-induced JNK activation was p53 independent. Hydrogen Peroxide 112-116 tumor protein p53 Homo sapiens 144-147 23499753-2 2013 H2O2 exposure reduced cellular proliferation similarly in both p53-290 and vector cells, and p53-290 cells demonstrating decreased cell viability at 1mM H2O2 (~85% viable). Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 63-66 23499753-2 2013 H2O2 exposure reduced cellular proliferation similarly in both p53-290 and vector cells, and p53-290 cells demonstrating decreased cell viability at 1mM H2O2 (~85% viable). Hydrogen Peroxide 153-157 tumor protein p53 Homo sapiens 93-96 23321474-4 2013 Nitric oxide activates the unfolded protein and heat shock responses and MAPK kinase signaling, whereas H2O2 stimulates p53 stabilization and poly(ADP-ribose) polymerase (PARP) activation but fails to induce the unfolded protein or heat shock responses or MAPK activation. Hydrogen Peroxide 104-108 tumor protein p53 Homo sapiens 120-123 23241309-5 2013 In response to hydrogen peroxide (H2O2), we measured an increase in PtdIns5P in cells derived from human osteosarcoma, U2OS (5-fold); breast tumors, MDA-MB-468 (2-fold); and fibrosarcoma, HT1080 (3-fold); and in p53-null murine embryonic fibroblasts (8-fold). Hydrogen Peroxide 15-32 tumor protein p53 Homo sapiens 212-215 23241309-5 2013 In response to hydrogen peroxide (H2O2), we measured an increase in PtdIns5P in cells derived from human osteosarcoma, U2OS (5-fold); breast tumors, MDA-MB-468 (2-fold); and fibrosarcoma, HT1080 (3-fold); and in p53-null murine embryonic fibroblasts (8-fold). Hydrogen Peroxide 34-38 tumor protein p53 Homo sapiens 212-215 22785213-4 2012 Treatment of MCF-7 cells with H2O2 caused an increase in this binding affinity while this same treatment of ZR-75-1 cells caused the p53 protein to lose binding affinity to several genes. Hydrogen Peroxide 30-34 tumor protein p53 Homo sapiens 133-136 22955948-6 2013 IKK-beta and p85 S6K1 contributed to H(2)O(2)-induced phosphorylation of Mdm2 (S166) and p53 accumulation. Hydrogen Peroxide 37-45 tumor protein p53 Homo sapiens 89-92 23203124-8 2012 This impairment of p53 suppressed the hydrogen peroxide-induced apoptotic response in hepatocytes. Hydrogen Peroxide 38-55 tumor protein p53 Homo sapiens 19-22 22750558-9 2012 p53 knock-down abrogated H(2)O(2)-induced premature senescence of vector control- and IkappaBalphaSR-expressing HDFs functionally linking canonical NF-kappaB-dependent control of p53 levels to ROS-induced HDF senescence. Hydrogen Peroxide 25-33 tumor protein p53 Homo sapiens 0-3 22750558-9 2012 p53 knock-down abrogated H(2)O(2)-induced premature senescence of vector control- and IkappaBalphaSR-expressing HDFs functionally linking canonical NF-kappaB-dependent control of p53 levels to ROS-induced HDF senescence. Hydrogen Peroxide 25-33 tumor protein p53 Homo sapiens 179-182 23235459-4 2012 In this study, we showed that under DNA damage conditions induced by chemotherapeutic drugs, gamma irradiation and hydrogen peroxide, p53 upregulates a novel protein, proline-rich acidic protein 1 (PRAP1). Hydrogen Peroxide 115-132 tumor protein p53 Homo sapiens 134-137 22454142-8 2012 NS-cultured cells exposed to H(2)O(2) showed significantly reduced cell viability (~50 %), increased p53 and caspase-3, and DNA fragmentation, without affecting ADNP expression. Hydrogen Peroxide 29-37 tumor protein p53 Homo sapiens 101-104 22785213-5 2012 The mutant p53 proteins from all cell lines had minimal to weak binding to these sequences even after treatment with H2O2. Hydrogen Peroxide 117-121 tumor protein p53 Homo sapiens 11-14 22514710-9 2012 In conclusion, the results indicate that H(2)O(2) at low concentrations causes premature senescence in human keratinocytes by activating p53-p21(CIP1) signaling and that these effects can be prevented by acute AMPK activation and enhanced by AMPK downregulation. Hydrogen Peroxide 41-49 tumor protein p53 Homo sapiens 137-140 23227799-7 2012 Our results show that HUVECs incubated with 200 muM H(2)O(2) had significantly decreased the viability of endothelial cells, which was accompanied with apparent cell apoptosis, the activation of caspase-3 and the upregulation of p53 expression, which was known to play a key role in H(2)O(2) -induced cell apoptosis. Hydrogen Peroxide 52-60 tumor protein p53 Homo sapiens 229-232 23259030-7 2012 Furthermore, EGCG was found to prevent serial passage- and H(2)O(2)-induced senescence in HDFs by suppressing p53 acetylation, but the Sirt1 activity was unaffected. Hydrogen Peroxide 59-67 tumor protein p53 Homo sapiens 110-113 22342995-4 2012 Consistent with these observations, the apoptotic pathway activated upon H2O2 treatment relies upon Trx1 oxidation, and is mediated by the p38(MAPK)/p53 signaling axis. Hydrogen Peroxide 73-77 tumor protein p53 Homo sapiens 149-152 23049256-6 2012 Under the same conditions, LMWP-SOD1 abolished activation of the cell cycle regulator proteins, p53 and p21(Cip1), induced by hydrogen peroxide. Hydrogen Peroxide 126-143 tumor protein p53 Homo sapiens 96-99 21516123-1 2011 Mammalian Ste20-like kinase-1 (MST1) kinase mediates H2O2-induced cell death by anticancer drugs such as cisplatin in a p53-dependent manner. Hydrogen Peroxide 53-57 tumor protein p53 Homo sapiens 120-123 21552291-6 2011 Both mouse and human cells lacking p53 are hypersensitive to hydrogen peroxide (H(2)O(2))-induced cell death compared with their isogenic wild-type counterparts. Hydrogen Peroxide 61-78 tumor protein p53 Homo sapiens 35-38 21552291-6 2011 Both mouse and human cells lacking p53 are hypersensitive to hydrogen peroxide (H(2)O(2))-induced cell death compared with their isogenic wild-type counterparts. Hydrogen Peroxide 80-88 tumor protein p53 Homo sapiens 35-38 21552291-8 2011 Although p53 and its classical targets such as p21 and Mdm2 are activated by both H(2)O(2) and CDDP, we found that the expression of haeme-oxygenase-1 (HO-1)-an antioxidant and antiapoptotic protein-was directly induced only upon H(2)O(2) treatment in a p53-dependent manner. Hydrogen Peroxide 82-90 tumor protein p53 Homo sapiens 9-12 21552291-8 2011 Although p53 and its classical targets such as p21 and Mdm2 are activated by both H(2)O(2) and CDDP, we found that the expression of haeme-oxygenase-1 (HO-1)-an antioxidant and antiapoptotic protein-was directly induced only upon H(2)O(2) treatment in a p53-dependent manner. Hydrogen Peroxide 230-238 tumor protein p53 Homo sapiens 9-12 21552291-11 2011 Finally, H(2)O(2)-mediated cell death was rescued significantly in p53-deficient cells by antioxidant treatment, as well as by bilirubin, a by-product of HO-1 metabolism. Hydrogen Peroxide 9-17 tumor protein p53 Homo sapiens 67-70 21909125-12 2011 H2O2 (100 mumol/L) significantly decreased Sirt1 expression, and induced up-regulation of p53 acetylation and p16(INK4a), which were blocked by pre-treatment with RHL (10 mumol/L). Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 90-93 20878061-3 2010 NEEP21 expression is inducible in non-neuronal human cancer cell lines by exposure to adriamycin, hydrogen peroxide, UV and gamma-ray in a p53-dependent manner. Hydrogen Peroxide 98-115 tumor protein p53 Homo sapiens 139-142 21950100-6 2011 It has been observed, that 6 h after exposure oflymphocytes to hydrogen peroxide and UV-light at doses 1510 and 3020 J/m2, the p53 level of investigated cells raises. Hydrogen Peroxide 63-80 tumor protein p53 Homo sapiens 127-130 21258766-4 2011 The dose- and time-dependent effect of H2O2 on Bax, p53 and Bcl-2 expression was assessed by Western blot analysis. Hydrogen Peroxide 39-43 tumor protein p53 Homo sapiens 52-55 21258766-5 2011 Treatment of cells with 1 mM H2O2 for 1 h induced an increase in Bax and p53 expression, but the expression of Bcl-2 was not affected by H2O2 treatment. Hydrogen Peroxide 29-33 tumor protein p53 Homo sapiens 73-76 21210663-4 2011 After a sandwich immunoreaction, the HRP-p53(392)Ab(2)-GO captured onto the electrode surface produced an amplified electrocatalytic response by the reduction of enzymatically oxidized thionine in the presence of hydrogen peroxide. Hydrogen Peroxide 213-230 tumor protein p53 Homo sapiens 41-44 21103079-3 2010 The level of p27 was decreased by hydrogen peroxide in a dose-dependent manner in human colon carcinoma HCT 116 (p53-positive) cells while it was increased upon exposure to hydrogen peroxide in HT 29 (p53-negative) cells. Hydrogen Peroxide 34-51 tumor protein p53 Homo sapiens 113-116 21103079-3 2010 The level of p27 was decreased by hydrogen peroxide in a dose-dependent manner in human colon carcinoma HCT 116 (p53-positive) cells while it was increased upon exposure to hydrogen peroxide in HT 29 (p53-negative) cells. Hydrogen Peroxide 173-190 tumor protein p53 Homo sapiens 201-204 19861417-7 2009 Persistent DHCR24 overexpression did not alter the phosphorylation status of p53 but resulted in decreased acetylation of p53 at lysine residues 373 and 382 in the nucleus after treatment with hydrogen peroxide. Hydrogen Peroxide 193-210 tumor protein p53 Homo sapiens 122-125 20845286-7 2010 BRCA1-IRIS abrogation of the homeostatic balance maintained by p38MAPK-p53-WIP1 pathway suppressed cell death induced by a lethal dose of UVC, high dosages of etoposide or H2O2, and allowed cells to survive and proliferate post geno-/cell-toxic stresses. Hydrogen Peroxide 172-176 tumor protein p53 Homo sapiens 71-74 20190405-9 2010 Induction of p53, the activation of caspase-3 by H(2)O(2) which accompanying downregulation of bcl-2, was blocked by CXC195. Hydrogen Peroxide 49-57 tumor protein p53 Homo sapiens 13-16 19641144-0 2009 Enhanced DNA binding capacity on up-regulated epidermal wild-type p53 in vitiligo by H2O2-mediated oxidation: a possible repair mechanism for DNA damage. Hydrogen Peroxide 85-89 tumor protein p53 Homo sapiens 66-69 19925028-3 2009 Treatment of MCF-7 cells having wild-type p53 with hydrogen peroxide increased the binding of p53 to DNA as detected using all three assays, but to different extents. Hydrogen Peroxide 51-68 tumor protein p53 Homo sapiens 42-45 19925028-3 2009 Treatment of MCF-7 cells having wild-type p53 with hydrogen peroxide increased the binding of p53 to DNA as detected using all three assays, but to different extents. Hydrogen Peroxide 51-68 tumor protein p53 Homo sapiens 94-97 18681908-8 2009 Activation of SIRT1 negatively regulates UV- and H(2)O(2)-induced p53 acetylation, because nicotinamide and sirtinol as well as SIRT1 siRNA enhance UV- and H(2)O(2)-induced p53 acetylation, whereas SIRT1 activator resveratrol inhibits it. Hydrogen Peroxide 49-57 tumor protein p53 Homo sapiens 66-69 19723085-7 2009 DHA and ALA inhibited DNA fragmentation, inhibited the decrease in cell viability, and inhibited the expression of apoptotic genes (p53, Bax, apoptosis-inducing factor) induced by hydrogen peroxide in pancreatic acinar cells. Hydrogen Peroxide 180-197 tumor protein p53 Homo sapiens 132-135 18681908-8 2009 Activation of SIRT1 negatively regulates UV- and H(2)O(2)-induced p53 acetylation, because nicotinamide and sirtinol as well as SIRT1 siRNA enhance UV- and H(2)O(2)-induced p53 acetylation, whereas SIRT1 activator resveratrol inhibits it. Hydrogen Peroxide 49-57 tumor protein p53 Homo sapiens 173-176 18681908-8 2009 Activation of SIRT1 negatively regulates UV- and H(2)O(2)-induced p53 acetylation, because nicotinamide and sirtinol as well as SIRT1 siRNA enhance UV- and H(2)O(2)-induced p53 acetylation, whereas SIRT1 activator resveratrol inhibits it. Hydrogen Peroxide 156-164 tumor protein p53 Homo sapiens 66-69 18681908-8 2009 Activation of SIRT1 negatively regulates UV- and H(2)O(2)-induced p53 acetylation, because nicotinamide and sirtinol as well as SIRT1 siRNA enhance UV- and H(2)O(2)-induced p53 acetylation, whereas SIRT1 activator resveratrol inhibits it. Hydrogen Peroxide 156-164 tumor protein p53 Homo sapiens 173-176 18708163-5 2008 In contrast, the caspase inhibitors, p53-siRNA, and Noxa-siRNA suppressed H(2)O(2)-induced cell death. Hydrogen Peroxide 74-82 tumor protein p53 Homo sapiens 37-40 19082758-0 2009 p53 and ATF-2 partly mediate the overexpression of COX-2 in H(2)O (2)-induced premature senescence of human fibroblasts. Hydrogen Peroxide 60-69 tumor protein p53 Homo sapiens 0-3 19627195-6 2009 Exposure of young HDFs to H(2)O(2) induced G2/M cell cycle arrest, positive senescence-associated (SA) beta-galactosidase (beta-gal) staining, and elevated p53, p21, and p16 protein levels. Hydrogen Peroxide 26-34 tumor protein p53 Homo sapiens 156-159 19627195-10 2009 In conclusion, dropwort showed a potential anti-senescence activity in H(2)O(2)-treated HDFs, which might be mediated by reducing p16, p21, and p53 levels and oxidative stress. Hydrogen Peroxide 71-79 tumor protein p53 Homo sapiens 144-147 19413947-6 2009 Finally, p53-depleted cells exhibit significant disruption of cellular ROS homeostasis, characterized by reduced mitochondrial and cellular superoxide levels and increased cellular hydrogen peroxide. Hydrogen Peroxide 181-198 tumor protein p53 Homo sapiens 9-12 19302811-6 2009 Treatment with 4 mM of H(2)O(2) for 24 or 96 h caused increase in Bax (56%, 227%), cytochrome c (282%, 701%), Smac/DIABLO (155%, 260%), caspase-3 protease activity (51%, 141%), and nuclear and cytosolic p53 (719%, 1581%) levels in the myotubes. Hydrogen Peroxide 23-31 tumor protein p53 Homo sapiens 203-206 18681908-0 2009 SIRT1 confers protection against UVB- and H2O2-induced cell death via modulation of p53 and JNK in cultured skin keratinocytes. Hydrogen Peroxide 42-46 tumor protein p53 Homo sapiens 84-87 19472297-3 2009 In this study, designed to elucidate the regulation of MVP in young and senescent HDFs, we examined the levels of transcriptional factors for the MVP gene, which revealed that among the putative transcriptional factors, p53 decreased only in young HDFs, but not in senescent HDFs in response to H(2)O(2) treatment in the same mode as the expression of MVP. Hydrogen Peroxide 295-303 tumor protein p53 Homo sapiens 220-223 19472297-4 2009 Moreover, the phosphorylation status of p53 increased only in senescent HDFs but not in young HDFs in response to H(2)O(2) treatment. Hydrogen Peroxide 114-122 tumor protein p53 Homo sapiens 40-43 18490454-6 2008 Moreover, Cdk5 (cyclin-dependent kinase 5), a proline-directed Ser/Thr kinase, additionally increases p53 stability via post-translational modification of p53 in response to hydrogen peroxide. Hydrogen Peroxide 174-191 tumor protein p53 Homo sapiens 102-105 18490454-6 2008 Moreover, Cdk5 (cyclin-dependent kinase 5), a proline-directed Ser/Thr kinase, additionally increases p53 stability via post-translational modification of p53 in response to hydrogen peroxide. Hydrogen Peroxide 174-191 tumor protein p53 Homo sapiens 155-158 18490454-9 2008 Our results show that c-Abl and Cdk5 cooperatively regulate maximal activation of p53, resulting in neuronal death in response to oxidative stress by hydrogen peroxide. Hydrogen Peroxide 150-167 tumor protein p53 Homo sapiens 82-85 18177348-10 2008 Finally, we propose a novel mechanism involving H2O2 in the regulation of tyrosinase via p53 through transcription of hepatocyte nuclear factor 1alpha which in turn can also affect the POMC response. Hydrogen Peroxide 48-52 tumor protein p53 Homo sapiens 89-92 18419600-3 2008 Firstly, HCT116 p53(+)/(+) and p53(-)/(-) colorectal cancer cells were treated with H(2)O(2) for 6 hrs and 24 hrs (early/late response). Hydrogen Peroxide 84-92 tumor protein p53 Homo sapiens 31-34 18155176-1 2008 Overexpression of the tumor suppressor gene, wild-type p53 (wtp53), using adenoviral vectors (Adp53) has been suggested to kill cancer cells by hydroperoxide-mediated oxidative stress [1,2] and nutrient distress induced by the glucose analog, 2-deoxyglucose (2DG), has been suggested to enhance tumor cell killing by agents that induce oxidative stress via disrupting hydroperoxide metabolism [3,4]. Hydrogen Peroxide 144-157 tumor protein p53 Homo sapiens 55-58 18155176-1 2008 Overexpression of the tumor suppressor gene, wild-type p53 (wtp53), using adenoviral vectors (Adp53) has been suggested to kill cancer cells by hydroperoxide-mediated oxidative stress [1,2] and nutrient distress induced by the glucose analog, 2-deoxyglucose (2DG), has been suggested to enhance tumor cell killing by agents that induce oxidative stress via disrupting hydroperoxide metabolism [3,4]. Hydrogen Peroxide 368-381 tumor protein p53 Homo sapiens 55-58 18060474-3 2008 The objective of the present study was to elucidate the effect of hydrogen peroxide (H2O2) on apoptotic signal molecules in vitro in SiHa and CaSki cell lines expressing the human papilloma virus 16 E6 protein, which causes the ubiquitin-mediated degradation of p53 protein and is thus p53 deficient. Hydrogen Peroxide 66-83 tumor protein p53 Homo sapiens 262-265 18060474-3 2008 The objective of the present study was to elucidate the effect of hydrogen peroxide (H2O2) on apoptotic signal molecules in vitro in SiHa and CaSki cell lines expressing the human papilloma virus 16 E6 protein, which causes the ubiquitin-mediated degradation of p53 protein and is thus p53 deficient. Hydrogen Peroxide 66-83 tumor protein p53 Homo sapiens 286-289 18060474-3 2008 The objective of the present study was to elucidate the effect of hydrogen peroxide (H2O2) on apoptotic signal molecules in vitro in SiHa and CaSki cell lines expressing the human papilloma virus 16 E6 protein, which causes the ubiquitin-mediated degradation of p53 protein and is thus p53 deficient. Hydrogen Peroxide 85-89 tumor protein p53 Homo sapiens 262-265 18060474-3 2008 The objective of the present study was to elucidate the effect of hydrogen peroxide (H2O2) on apoptotic signal molecules in vitro in SiHa and CaSki cell lines expressing the human papilloma virus 16 E6 protein, which causes the ubiquitin-mediated degradation of p53 protein and is thus p53 deficient. Hydrogen Peroxide 85-89 tumor protein p53 Homo sapiens 286-289 18025460-8 2007 This DNA-mediated oxidation of p53 parallels that seen by treating cells with hydrogen peroxide. Hydrogen Peroxide 78-95 tumor protein p53 Homo sapiens 31-34 18160328-12 2008 Furthermore, CQ inhibited H2O2-mediated up-regulation of p53 activity in the M17 cells and this was dependent on PI3K activation. Hydrogen Peroxide 26-30 tumor protein p53 Homo sapiens 57-60 18202783-3 2008 The data obtained show that normal human lymphocytes exposed in vitro to known DNA-damaging agents, e.g. H2O2, ionizing radiation and mitomycin C, exhibit an asynchronous replication of the genes TP53 and RB1. Hydrogen Peroxide 105-109 tumor protein p53 Homo sapiens 196-200 18154926-12 2008 On the other hand, H(2)O(2) induces long-term p53 expression, late expression of PAK1 without activation of p21 promoter. Hydrogen Peroxide 19-27 tumor protein p53 Homo sapiens 46-49 17567683-0 2007 Role of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure. Hydrogen Peroxide 86-90 tumor protein p53 Homo sapiens 8-11 17951408-3 2007 The down-regulated expression of hOGG1 by its small interfering RNA prominently triggers the H(2)O(2)-induced apoptosis in human fibroblasts GM00637 and human lung carcinoma H1299 cells via the p53-mediated apoptotic pathway. Hydrogen Peroxide 93-101 tumor protein p53 Homo sapiens 194-197 17951408-5 2007 The p53-small interfering RNA transfection into the hOGG1-deficient GM00637 markedly inhibited the H(2)O(2)-induced activation of p53-downstream target proteins such as p21, Noxa, and caspase-3/7, which eventually resulted in the increased cell viability. Hydrogen Peroxide 99-107 tumor protein p53 Homo sapiens 4-7 17951408-5 2007 The p53-small interfering RNA transfection into the hOGG1-deficient GM00637 markedly inhibited the H(2)O(2)-induced activation of p53-downstream target proteins such as p21, Noxa, and caspase-3/7, which eventually resulted in the increased cell viability. Hydrogen Peroxide 99-107 tumor protein p53 Homo sapiens 130-133 17951408-8 2007 Therefore, we suggest that although p53 is a major modulator of apoptosis, hOGG1 also plays a pivotal role in protecting cells against the H(2)O(2)-induced apoptosis at the upstream of the p53-dependent pathway to confer a survival advantage to human fibroblasts and human lung carcinomas through maintaining their genomic stability. Hydrogen Peroxide 139-147 tumor protein p53 Homo sapiens 189-192 17595514-0 2007 H2O2 accelerates cellular senescence by accumulation of acetylated p53 via decrease in the function of SIRT1 by NAD+ depletion. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 67-70 17567683-6 2007 Under mild or severe H2O2-induced stress, p53-deficient SiHa cells exhibited much higher ROS levels than control SiHa cells. Hydrogen Peroxide 21-25 tumor protein p53 Homo sapiens 42-45 17567683-7 2007 Furthermore, we analyzed cell viability and apoptosis after H2O2 treatment and found that p53 deficiency sensitized SiHa cells to H2O2 damage. Hydrogen Peroxide 60-64 tumor protein p53 Homo sapiens 90-93 17567683-7 2007 Furthermore, we analyzed cell viability and apoptosis after H2O2 treatment and found that p53 deficiency sensitized SiHa cells to H2O2 damage. Hydrogen Peroxide 130-134 tumor protein p53 Homo sapiens 90-93 17567683-8 2007 Inhibition of p53 resulted in excessive oxidation of DNA; control SiHa cells exhibited a more rapid removal of 8-oxo-7,8-dihydro-2"-deoxyguanosine from DNA compared with p53-deficient SiHa cells exposed to the same level of H2O2 challenge. Hydrogen Peroxide 224-228 tumor protein p53 Homo sapiens 14-17 16508959-11 2006 Caveolin 1 induction and stresses with both IL-1beta and H2O2 up-regulated p53 and p21 and down-regulated phosphorylated retinoblastoma (Rb), suggesting that the p53/p21/Rb phosphorylation pathway, as well as prolonged p38 MAPK activation, may mediate the features of chondrocyte senescence induced by stress. Hydrogen Peroxide 57-61 tumor protein p53 Homo sapiens 75-78 16829532-8 2006 H2O2 in bolus or generated from the dismutation of O2- by SOD, was cytotoxic at high concentrations and activated p53 independent of NO. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 114-117 16797759-3 2006 By Western blot, the expression of 14-3-3sigma, p53 and p21 was coordinately upregulated in astrocytes following exposure to hydrogen peroxide, 4-hydroxy-2-nonenal (4-HNE) or etoposide, a topoisomerase II inhibitor. Hydrogen Peroxide 125-142 tumor protein p53 Homo sapiens 48-51 17012228-10 2006 In addition, H2O2 treatment of untransfected cells caused an increase in p53 sumoylation by SUMO-2/3, whereas that by SUMO-1 remained unchanged. Hydrogen Peroxide 13-17 tumor protein p53 Homo sapiens 73-76 16785031-7 2006 Although SIRT1 did not affect H(2)O(2)-mediated phosphorylation of mitogen-activated protein (MAP) kinase, it interacted with p53 and inhibited H(2)O(2)-mediated p53 acetylation but not phosphorylation in MMCs. Hydrogen Peroxide 144-152 tumor protein p53 Homo sapiens 162-165 16508959-11 2006 Caveolin 1 induction and stresses with both IL-1beta and H2O2 up-regulated p53 and p21 and down-regulated phosphorylated retinoblastoma (Rb), suggesting that the p53/p21/Rb phosphorylation pathway, as well as prolonged p38 MAPK activation, may mediate the features of chondrocyte senescence induced by stress. Hydrogen Peroxide 57-61 tumor protein p53 Homo sapiens 162-165 16288013-2 2005 Here, we describe the gene expression profiles associated with four microenvironmental components of the inflammatory response (NO*, H2O2, DNA replication arrest, and hypoxia) that result in p53 stabilization and activation. Hydrogen Peroxide 133-137 tumor protein p53 Homo sapiens 191-194 16393695-0 2006 Insulin-like growth factor-1 prevents Abeta[25-35]/(H2O2)- induced apoptosis in lymphocytes by reciprocal NF-kappaB activation and p53 inhibition via PI3K-dependent pathway. Hydrogen Peroxide 52-56 tumor protein p53 Homo sapiens 131-134 15728246-2 2005 In our study, we characterized the signaling pathway linking oxidative stress induced by sublethal concentrations of H2O2 to p53 in primary human endothelial cells through the interferon (IFN)-inducible gene IFI16. Hydrogen Peroxide 117-121 tumor protein p53 Homo sapiens 125-128 15809720-13 2005 These findings indicate that hydrogen peroxide overload on p53-independent pathway due to MnSOD overexpression plus BSO might increase the apoptosis frequency without acceleration of apoptotic process of each cell, resulting in negating ADR-tolerance of MnSOD-overexpressing cell lines. Hydrogen Peroxide 29-46 tumor protein p53 Homo sapiens 59-62 15573749-5 2004 Both TK6 and WTK1 cells showed effective post-damage repair capacity in incubation time of 0.5 h to 1 h. The background level of P53 protein in WTK1 was higher than that in TK6, but the level of P53 protein in H2O2-treated TK6 increased significantly higher than that in H2O2-treated WTK1. Hydrogen Peroxide 210-214 tumor protein p53 Homo sapiens 129-132 15573749-5 2004 Both TK6 and WTK1 cells showed effective post-damage repair capacity in incubation time of 0.5 h to 1 h. The background level of P53 protein in WTK1 was higher than that in TK6, but the level of P53 protein in H2O2-treated TK6 increased significantly higher than that in H2O2-treated WTK1. Hydrogen Peroxide 271-275 tumor protein p53 Homo sapiens 129-132 15795422-7 2005 In contrast, cells treated with apigenin remained DCF and phospho-p53 staining positive in response to H2O2. Hydrogen Peroxide 103-107 tumor protein p53 Homo sapiens 66-69 15489061-8 2004 Both cell lines displayed a higher DNA-binding activity of p53 and HIF-1 72 h after H2O2 exposure. Hydrogen Peroxide 84-88 tumor protein p53 Homo sapiens 59-62 15489061-9 2004 Our results indicate that similar mechanisms involving p21(WAF-1) and probably p53 are at work in BJ and hTERT-BJ1 HDFs under H2O2-induced SIPS, suggesting that generalized DNA damage rather than telomere length/telomerase plays a crucial role in H2O2induced SIPS. Hydrogen Peroxide 126-130 tumor protein p53 Homo sapiens 79-82 15260130-3 2004 H2O2 increased both the ratio of bax/bcl-2 and the p53 mRNA expression. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 51-54 15081873-4 2004 Similarly, the cell-permeable SN50 peptide, which is known to block NF-kappaB nuclear translocation, prevented both H(2)O(2)-induced p53 expression and apoptosis. Hydrogen Peroxide 116-124 tumor protein p53 Homo sapiens 133-136 15163458-11 2004 CONCLUSIONS: This study provides evidence that redox-active (Fe2+), (Mn2+), (Cu2+), and (Zn2+) ion-induced apoptosis in PBL by (H2O2)/(.OH) generation, resulting in mitochondria depolarization, caspase-3 activation, and nuclear fragmentation independent of NF-kappaB and p53 transcription factors activation. Hydrogen Peroxide 128-132 tumor protein p53 Homo sapiens 271-274 14637145-0 2003 Hydrogen peroxide increases a 55-kDa tyrosinase concomitantly with induction of p53-dependent p21 waf1 expression and a greater Bax/Bcl-2 ratio in pigmented melanoma. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 80-83 12959929-2 2004 Although H2O2 and other peroxides have been shown to induce ataxia telangiectasia-mutated (ATM)-dependent p53 phosphorylation in response to DNA damage, the signal transduction mechanisms in response to hyperoxia are currently unknown. Hydrogen Peroxide 9-13 tumor protein p53 Homo sapiens 106-109 12959929-12 2004 Together, our data suggest that hyperoxia activates the ATR-Chk1 pathway and phosphorylates p53 at multiple sites in an ATM-independent manner, which is different from other forms of oxidative stress such as H2O2 or UV light. Hydrogen Peroxide 208-212 tumor protein p53 Homo sapiens 92-95 14555612-7 2004 Doxorubicin and hydrogen peroxide (H2O2) treatment, although inducing p53 stabilization, did not cause the activation of glycosylase. Hydrogen Peroxide 16-33 tumor protein p53 Homo sapiens 70-73 14555612-7 2004 Doxorubicin and hydrogen peroxide (H2O2) treatment, although inducing p53 stabilization, did not cause the activation of glycosylase. Hydrogen Peroxide 35-39 tumor protein p53 Homo sapiens 70-73 14637145-5 2003 Together, these data suggest that response to hydrogen peroxide involves p53-mediated growth-restrictive signaling and unequal induction of specific DOPA oxidases in melanocytic cells with unequal basal pigmentation. Hydrogen Peroxide 46-63 tumor protein p53 Homo sapiens 73-76 12962703-1 2003 We had earlier shown that higher concentration of hydrogen peroxide (H(2)O(2)) induced p53-dependent apoptosis in glioma cell line with wild type p53 but had minimal effect on cells with mutated p53. Hydrogen Peroxide 50-67 tumor protein p53 Homo sapiens 87-90 12962703-1 2003 We had earlier shown that higher concentration of hydrogen peroxide (H(2)O(2)) induced p53-dependent apoptosis in glioma cell line with wild type p53 but had minimal effect on cells with mutated p53. Hydrogen Peroxide 50-67 tumor protein p53 Homo sapiens 146-149 12962703-1 2003 We had earlier shown that higher concentration of hydrogen peroxide (H(2)O(2)) induced p53-dependent apoptosis in glioma cell line with wild type p53 but had minimal effect on cells with mutated p53. Hydrogen Peroxide 50-67 tumor protein p53 Homo sapiens 146-149 12962703-1 2003 We had earlier shown that higher concentration of hydrogen peroxide (H(2)O(2)) induced p53-dependent apoptosis in glioma cell line with wild type p53 but had minimal effect on cells with mutated p53. Hydrogen Peroxide 69-77 tumor protein p53 Homo sapiens 87-90 12962703-1 2003 We had earlier shown that higher concentration of hydrogen peroxide (H(2)O(2)) induced p53-dependent apoptosis in glioma cell line with wild type p53 but had minimal effect on cells with mutated p53. Hydrogen Peroxide 69-77 tumor protein p53 Homo sapiens 146-149 12962703-1 2003 We had earlier shown that higher concentration of hydrogen peroxide (H(2)O(2)) induced p53-dependent apoptosis in glioma cell line with wild type p53 but had minimal effect on cells with mutated p53. Hydrogen Peroxide 69-77 tumor protein p53 Homo sapiens 146-149 12890678-6 2003 Similarly, p53 activation by H2O2 was inhibited by kinase-inactive forms of the PDGF beta receptor or ATM. Hydrogen Peroxide 29-33 tumor protein p53 Homo sapiens 11-14 12890678-3 2003 In response to H2O2, we observed phosphorylation of p53 specifically at serine 15, but not serine 9, 20, or 392. Hydrogen Peroxide 15-19 tumor protein p53 Homo sapiens 52-55 12890678-5 2003 We found that H2O2 induced phosphorylation of the PDGF beta receptor and increased ATM kinase activity, two events integral to p53 activation as either AG1433 (a PDGF beta receptor inhibitor) or caffeine (an ATM kinase inhibitor) inhibited Ser-15 phosphorylation. Hydrogen Peroxide 14-18 tumor protein p53 Homo sapiens 127-130 12935767-8 2003 The induction and nuclear translocation of p53 by H2O2 was blocked by pyruvate and appeared to be somewhat enhanced by L-lactate or aminooxyacetate in association with oxidant generation. Hydrogen Peroxide 50-54 tumor protein p53 Homo sapiens 43-46 12935767-10 2003 The pyruvate-related redox manipulation inhibited the H2O2-induced p53 activation, restored the downregulated bcl-2 and the upregulated bax, and hence enhanced the bcl-2/bax expression ratio. Hydrogen Peroxide 54-58 tumor protein p53 Homo sapiens 67-70 12805649-12 2003 These results demonstrate that, in this cell lineage, sensitivity to oxidative DNA damage by H2O2 decreases with tumorigenicity (i.e., MCF-10A vs. MCF-10ATG3B), and show that DNA repair, altered Ras, and p53 expression, or compensatory mechanisms involving elevated antioxidant enzymes are involved in mediating these effects. Hydrogen Peroxide 93-97 tumor protein p53 Homo sapiens 204-207 12890678-9 2003 Thus, these data link PDGF beta receptor transactivation to H2O2-induced p53 phosphorylation and suggest a functional role for growth factor receptors in modulation of p53 function. Hydrogen Peroxide 60-64 tumor protein p53 Homo sapiens 73-76 12213802-2 2002 H2O2 produced early activation of Akt/PKB and also DNA damage that was followed by stabilization of p53 levels, formation of reactive oxygen species (ROS), and generation of ceramide through activation of a glutathione-sensitive neutral sphingomyelinase. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 100-103 12468545-1 2003 Oxidants such as H(2)O(2) play a role in the toxicity of certain DNA-damaging agents, a process that often involves the tumor suppressor p53. Hydrogen Peroxide 17-25 tumor protein p53 Homo sapiens 137-140 12939400-4 2003 It was found that while alkylating agents induced both apoptosis and phosphorylation of the Ser-15 site of p53 in a MLH1-dependent manner, induction of apoptosis, but not p53 phosphorylation, was MLH1 dependent following treatment with H2O2. Hydrogen Peroxide 236-240 tumor protein p53 Homo sapiens 107-110 12768477-8 2003 These results suggest that induction of p53 by ALLN may be potentially important for triggering H(2)O(2)-induced apoptosis processes in RA synovial cells. Hydrogen Peroxide 96-104 tumor protein p53 Homo sapiens 40-43 12435813-7 2002 We demonstrate that PKCzeta inhibited p53/p56 Lyn phosphorylation and stimulation in drug- or H(2)O(2)-treated cells, suggesting that p53/p56 Lyn redox regulation is altered in PKCzeta-overexpressing cells. Hydrogen Peroxide 94-102 tumor protein p53 Homo sapiens 134-137 12397013-0 2002 Hydrogen peroxide induces upregulation of Fas in human airway epithelial cells via the activation of PARP-p53 pathway. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 106-109 12397013-9 2002 Inhibitors of poly(ADP-ribose) polymerase abrogated the H(2)O(2)-induced Fas translocation to the plasma membrane and p53 activation. Hydrogen Peroxide 56-64 tumor protein p53 Homo sapiens 118-121 12397013-11 2002 These results indicate that H(2)O(2) induces Fas upregulation by promoting cytoplasmic transport of Fas to the cell surface in human airway epithelial cells, and that the activation of the poly(ADP-ribose) polymerase-p53 pathway may be involved in this mechanism. Hydrogen Peroxide 28-36 tumor protein p53 Homo sapiens 217-220 11809417-0 2002 p53 dependent apoptosis in glioma cell lines in response to hydrogen peroxide induced oxidative stress. Hydrogen Peroxide 60-77 tumor protein p53 Homo sapiens 0-3 12160929-4 2002 The results showed that exposure to vapor TCE and PERC produced a dose-dependent and more pronounced accumulation of H(2)O(2) in p53-WT H460 than p53-null H1299 cells. Hydrogen Peroxide 117-125 tumor protein p53 Homo sapiens 129-132 12160929-5 2002 The accumulation of H(2)O(2) was accompanied by severe cellular damage, as indicated by the significant increase of lipid peroxidation and apoptosis in p53-WT H460 cells, but not p53-null H1299 cells. Hydrogen Peroxide 20-28 tumor protein p53 Homo sapiens 152-155 11880544-3 2002 Moreover, p53 also participates in base excision repair of hydrogen peroxide-induced damage, still at an early stage of investigation. Hydrogen Peroxide 59-76 tumor protein p53 Homo sapiens 10-13 11809417-10 2002 The U373MG cell line, having mutated p53, was comparatively resistant to H(2)O(2) induced cell death. Hydrogen Peroxide 73-81 tumor protein p53 Homo sapiens 37-40 11673079-0 2001 The detection of mutations induced in vitro in the human p53 gene by hydrogen peroxide with the restriction site mutation (RSM) assay. Hydrogen Peroxide 69-86 tumor protein p53 Homo sapiens 57-60 11673079-1 2001 We have analysed five mutation hotspots within the p53 gene (codons 175, 213, 248, 249, and 282) for mutations induced by hydrogen peroxide (H(2)O(2)), employing the restriction site mutation (RSM) assay. Hydrogen Peroxide 122-139 tumor protein p53 Homo sapiens 51-54 11313880-3 2001 p53 activation was followed by the formation of reactive oxygen species (ROS), superoxide anion (O2-*) measured by hydroethidium oxidation into ethidium and hydrogen peroxide (H2O2) measured by oxidation of 2",7"-dichlorofluorescin (DCFH) into 2",7"-dichlorofluorescein (DCF), which was accompanied with ceramide generation through the activation of neutral, but not acid, sphingomyelinase. Hydrogen Peroxide 157-174 tumor protein p53 Homo sapiens 0-3 11447225-1 2001 Upon exposure of cells to hydrogen peroxide (H(2)O(2)) phosphorylation of p53 was rapidly induced in human fibroblast GM00637, and this phosphorylation occurred on serine 9, serine 15, serine 20, but not on serine 392. Hydrogen Peroxide 26-43 tumor protein p53 Homo sapiens 74-77 11447225-2 2001 In addition, H(2)O(2)-induced phosphorylation of p53 was followed by induction of p21, suggesting functional activation of p53. Hydrogen Peroxide 13-21 tumor protein p53 Homo sapiens 49-52 11447225-2 2001 In addition, H(2)O(2)-induced phosphorylation of p53 was followed by induction of p21, suggesting functional activation of p53. Hydrogen Peroxide 13-21 tumor protein p53 Homo sapiens 123-126 11744405-13 2001 However, permanent H(2)O(2) levels induce the tumour suppressor p53 which in turn downregulates cytosolic TR. Hydrogen Peroxide 19-27 tumor protein p53 Homo sapiens 64-67 11313880-3 2001 p53 activation was followed by the formation of reactive oxygen species (ROS), superoxide anion (O2-*) measured by hydroethidium oxidation into ethidium and hydrogen peroxide (H2O2) measured by oxidation of 2",7"-dichlorofluorescin (DCFH) into 2",7"-dichlorofluorescein (DCF), which was accompanied with ceramide generation through the activation of neutral, but not acid, sphingomyelinase. Hydrogen Peroxide 176-180 tumor protein p53 Homo sapiens 0-3 11020329-6 2000 Preparative gel electrophoresis of the PGK1 and p53 genes from 300 microg of restriction endonuclease-digested genomic DNA showed a 25-fold enrichment for the genes and, after endonuclease digestion followed by LMPCR, gave sufficient signal to map the frequency of oxidized bases from human cells treated with 50 microM H2O2. Hydrogen Peroxide 320-324 tumor protein p53 Homo sapiens 48-51 12214088-5 2000 H2O2-treatment resulted in dose-dependent increases in cell death due to genomic and mitochondrial DNA damage associated with increased levels of 8-OHdG and the p53 and CD95 pro-apoptosis genes, reduced levels of the Bcl-2 survival gene, activation of JNK and p38 stress kinases, and inhibition of PI3 kinase survival signaling. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 161-164 10922372-0 2000 Vanadate induces p53 transactivation through hydrogen peroxide and causes apoptosis. Hydrogen Peroxide 45-62 tumor protein p53 Homo sapiens 17-20 10922372-9 2000 These results demonstrate that vanadate induces p53 activation mainly through H(2)O(2) generation, and this activation is required for vanadate-induced apoptosis. Hydrogen Peroxide 78-86 tumor protein p53 Homo sapiens 48-51 10939520-0 2000 Hydrogen peroxide-induced apoptosis in human hepatoma cells is mediated by CD95(APO-1/Fas) receptor/ligand system and may involve activation of wild-type p53. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 154-157 10749685-0 2000 Apoptosis or senescence-like growth arrest: influence of cell-cycle position, p53, p21 and bax in H2O2 response of normal human fibroblasts. Hydrogen Peroxide 98-102 tumor protein p53 Homo sapiens 78-81 10939520-3 2000 Analysis of flow cytometry indicated that H2O2 can decrease the level of CD95(APO-1/Fas), and it is confirmed that H2O2 can also activate the differential expression of some specific gene such as p53 by means of RT-PCR technique. Hydrogen Peroxide 42-46 tumor protein p53 Homo sapiens 196-199 10939520-3 2000 Analysis of flow cytometry indicated that H2O2 can decrease the level of CD95(APO-1/Fas), and it is confirmed that H2O2 can also activate the differential expression of some specific gene such as p53 by means of RT-PCR technique. Hydrogen Peroxide 115-119 tumor protein p53 Homo sapiens 196-199 10939520-4 2000 The results indicated that CD95 signal transduction system may be involved in the H2O2-induced apoptosis, and can regulate some specific genes associated with apoptosis in transcription and translation levels such as p53. Hydrogen Peroxide 82-86 tumor protein p53 Homo sapiens 217-220 11708215-0 2000 Measurements of hydrogen peroxide induced premature senescence: senescence-associated beta-galactosidase and DNA synthesis index in human diploid fibroblasts with down-regulated p53 or Rb. Hydrogen Peroxide 16-33 tumor protein p53 Homo sapiens 178-181 10893182-3 2000 As hydrogen peroxide and other oxidants can affect signal transduction pathways at several levels, the present study was aimed to verify: (i) the occurrence of GGT-dependent production of hydrogen peroxide in melanoma cells; (ii) the effects of GGT-dependent prooxidant reactions on known redox-sensitive cellular targets, i.e. protein thiols, the nuclear transcription factor NF-kappa B and p53. Hydrogen Peroxide 188-205 tumor protein p53 Homo sapiens 392-395 10911952-9 2000 By inducing premature senescence with a pulse treatment of H2O2, we can study the role of the cell cycle checkpoint proteins p53, p21, p16 and Rb in gaining each feature of senescence. Hydrogen Peroxide 59-63 tumor protein p53 Homo sapiens 125-128 9890630-8 1999 In addition, p53-deficient astrocytes were more resistant to H2O2-induced apoptosis than wild-type and heterozygous astrocytes. Hydrogen Peroxide 61-65 tumor protein p53 Homo sapiens 13-16 10531375-4 1999 Cadmium at 10-30 microM impairs the p53 induction in response to DNA-damaging agents such as actinomycin D, methylmethane sulfonate, and hydrogen peroxide. Hydrogen Peroxide 137-154 tumor protein p53 Homo sapiens 36-39 10359739-5 1999 Both H2O2- and O2--induced apoptosis of cardiomyocytes were associated with an increase in p53 protein content, whereas protein levels of Bax and Bcl-2 were unaltered. Hydrogen Peroxide 5-9 tumor protein p53 Homo sapiens 91-94 10403664-0 1999 Methyl methanesulfonate and hydrogen peroxide differentially regulate p53 accumulation in hepatoblastoma cells. Hydrogen Peroxide 28-45 tumor protein p53 Homo sapiens 70-73 10403664-5 1999 At non-lethal doses, both H2O2 and MMS induced high level of p53 protein accumulation. Hydrogen Peroxide 26-30 tumor protein p53 Homo sapiens 61-64 10403664-6 1999 Nevertheless, while the amount of p53 protein increased with the dose of MMS and the occurrence of apoptotic cell death events, H2O2 doses that induce cell apoptosis attenuated the p53 protein accumulation level. Hydrogen Peroxide 128-132 tumor protein p53 Homo sapiens 181-184 9989825-9 1999 This detrimental effect of NF-kappaB mediating hydrogen peroxide-induced cell death presumably relies on the induced expression of death effector genes such as p53, which was NF-kappaB-dependently upregulated in the presence of H2O2. Hydrogen Peroxide 47-64 tumor protein p53 Homo sapiens 160-163 9989825-9 1999 This detrimental effect of NF-kappaB mediating hydrogen peroxide-induced cell death presumably relies on the induced expression of death effector genes such as p53, which was NF-kappaB-dependently upregulated in the presence of H2O2. Hydrogen Peroxide 228-232 tumor protein p53 Homo sapiens 160-163 9890630-0 1999 Hydrogen peroxide-induced apoptosis mediated by p53 protein in glial cells. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 48-51 9890630-5 1999 After treatment with H2O2, p53 protein was highly expressed and protein levels of Bak, p21WAF1/CIP1 and GADD45 were also enhanced. Hydrogen Peroxide 21-25 tumor protein p53 Homo sapiens 27-30 9535218-2 1998 We found that induction of apoptosis in Mphi by oxLDL, C2-ceramide, tumor necrosis factor alpha (TNF-alpha), and hydrogen peroxide (H2O2) was associated with enhanced expression of manganese superoxide dismutase (MnSOD) and p53. Hydrogen Peroxide 132-136 tumor protein p53 Homo sapiens 224-227 9576849-0 1998 Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts: p53 and Rb control G1 arrest but not cell replication. Hydrogen Peroxide 22-26 tumor protein p53 Homo sapiens 93-96 9576849-3 1998 To understand the molecular basis for the H2O2-induced growth arrest, we determined the cell cycle distribution, levels of p53 tumour suppressor and p21 cyclin-dependent kinase inhibitor proteins, and the status of Rb phosphorylation in H2O2-treated cells. Hydrogen Peroxide 42-46 tumor protein p53 Homo sapiens 123-126 9576849-6 1998 The level of p53 protein increased 2- to 3-fold within 1.5 h after H2O2 exposure but returned to the control level by 48 h. The induction of p53 protein was dose dependent, beginning at 50-75 microM and reaching a maximum at 100-250 microM. Hydrogen Peroxide 67-71 tumor protein p53 Homo sapiens 13-16 9576849-6 1998 The level of p53 protein increased 2- to 3-fold within 1.5 h after H2O2 exposure but returned to the control level by 48 h. The induction of p53 protein was dose dependent, beginning at 50-75 microM and reaching a maximum at 100-250 microM. Hydrogen Peroxide 67-71 tumor protein p53 Homo sapiens 141-144 9576849-10 1998 The induction of p53 by H2O2 was abolished by the iron chelator deferoxamine and the protein synthesis inhibitor cycloheximide. Hydrogen Peroxide 24-28 tumor protein p53 Homo sapiens 17-20 9576849-14 1998 Thus H2O2-treated cells show a transient elevation of p53, high level of p21, lack of Rb phosphorylation, G1 arrest and inability to replicate when G1 arrest is inactivated. Hydrogen Peroxide 5-9 tumor protein p53 Homo sapiens 54-57 9688948-4 1998 We found that three mesothelioma cell lines (1 with wild-type p53) were highly resistant to apoptosis induced by oxidant stimuli (asbestos, H2O2) or nonoxidant stimuli (calcium ionophore) compared with primary cultured mesothelial cells. Hydrogen Peroxide 140-144 tumor protein p53 Homo sapiens 62-65 9535218-3 1998 Treatment of cells with p53 or MnSOD antisense oligonucleotides prior to stimulation with oxLDL, C2-ceramide, TNF-alpha, or H2O2 caused an inhibition of the expression of the respective protein together with a marked reduction of apoptosis. Hydrogen Peroxide 124-128 tumor protein p53 Homo sapiens 24-27 9535218-4 1998 Exposure to N-acetylcysteine before treatment with oxLDL, C2-ceramide, TNF-alpha, or H2O2 reversed a decrease in cellular glutathione concentrations as well as the enhanced production of p53 and MnSOD mRNA and protein. Hydrogen Peroxide 85-89 tumor protein p53 Homo sapiens 187-190 9257692-4 1997 Here I show that p53 in cultured human skin fibroblasts is elevated after treatment with hydrogen peroxide, an oxidant produced in cells during exposure to solar UV radiation. Hydrogen Peroxide 89-106 tumor protein p53 Homo sapiens 17-20 7790313-3 1995 Immunostaining revealed nuclear accumulation of p53 within 6 h after various stresses [heat shock, osmotic shock, heavy metal (Cd), blockers of the cellular respiratory system (NaN3), amino acid analogues (azetidine and canavanine), an inhibitor of protein synthesis (puromycin), and oxygen free radicals (H2O2)]. Hydrogen Peroxide 306-310 tumor protein p53 Homo sapiens 48-51 9092641-8 1997 Hydrogen peroxide treatment of cells cotransfected with p53 results in a marked decrease in CAT activity, suggesting that oxidation of p53 decreases the ability of the protein to bind to consensus DNA and transactivate target genes in vivo. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 56-59 9092641-8 1997 Hydrogen peroxide treatment of cells cotransfected with p53 results in a marked decrease in CAT activity, suggesting that oxidation of p53 decreases the ability of the protein to bind to consensus DNA and transactivate target genes in vivo. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 135-138 8798554-9 1996 Exogenously added H2O2 increased p58(c-fgr) and p53/56(lyn) activities in nonadherent neutrophils. Hydrogen Peroxide 18-22 tumor protein p53 Homo sapiens 48-51 9799559-4 1998 The increase in p53 required the myeloperoxidase-dependent generation of hypochlorous acid and could be mimicked by exposing cells to a flux of hypochlorous acid produced by purified myeloperoxidase and a hydrogen peroxide-generating system. Hydrogen Peroxide 205-222 tumor protein p53 Homo sapiens 16-19 8036011-3 1994 We have studied the capacity of hydrogen peroxide plus ferric chloride (FeCl3) to induce base pair changes in the hotspot codons 248 and 249 of the p53 tumor suppressor gene in human fibroblasts. Hydrogen Peroxide 32-49 tumor protein p53 Homo sapiens 148-151 8036011-7 1994 It is evident that H2O2/FeCl3 possesses essentially the same mutagenic specificity for codons 249 and 250 of p53 as bulky carcinogens such as aflatoxin B1, benzo(a)pyrene or heterocyclic amines. Hydrogen Peroxide 19-23 tumor protein p53 Homo sapiens 109-112 8485705-2 1993 Activation of p53-DNA binding was seen for treatment with radiation, hydrogen peroxide, actinomycin D, Adriamycin, etoposide, camptothecin, 5-fluorouracil, mitomycin C, and cisplatin. Hydrogen Peroxide 69-86 tumor protein p53 Homo sapiens 14-17 33798680-7 2021 As the p53-specific adaptor of the nuclear import, KPNA4 upregulated with the same pattern of p53 in hydrogen peroxide-induced apoptosis in human lens epithelia cells. Hydrogen Peroxide 101-118 tumor protein p53 Homo sapiens 7-10 33798680-7 2021 As the p53-specific adaptor of the nuclear import, KPNA4 upregulated with the same pattern of p53 in hydrogen peroxide-induced apoptosis in human lens epithelia cells. Hydrogen Peroxide 101-118 tumor protein p53 Homo sapiens 94-97 9799559-8 1998 Hydrogen peroxide alone also elevated p53 and WAF1/CIP1, but the fluxes required were nearly 10-fold higher than those that were effective for hypochlorous acid. Hydrogen Peroxide 0-17 tumor protein p53 Homo sapiens 38-41 34144191-1 2021 Stabilization and activation of the p53 tumor suppressor are triggered in response to various cellular stresses, including DNA damaging agents and elevated Reactive Oxygen Species (ROS) like H2O2. Hydrogen Peroxide 191-195 tumor protein p53 Homo sapiens 36-39 34085157-9 2021 The mechanism for down-regulation of p53 expression in MCF-7iPOX cells was found at the level of p53-PEPD complex formation that was counteracted by hydrogen peroxide treatment. Hydrogen Peroxide 149-166 tumor protein p53 Homo sapiens 37-40 34085157-9 2021 The mechanism for down-regulation of p53 expression in MCF-7iPOX cells was found at the level of p53-PEPD complex formation that was counteracted by hydrogen peroxide treatment. Hydrogen Peroxide 149-166 tumor protein p53 Homo sapiens 97-100 34719737-5 2021 VAP-1 generated hydrogen peroxide acts via the p53 signaling pathway to regulate HSC proliferation. Hydrogen Peroxide 16-33 tumor protein p53 Homo sapiens 47-50 34855076-1 2021 Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 muM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53+ cells (by 40.2%) induced by exposure to H2O2 (60 muM). Hydrogen Peroxide 271-275 tumor protein p53 Homo sapiens 226-229 34855076-3 2021 Incubation of pulmonary fibroblasts culture with peptide G (Phe-D-Ala-Gly-Phe-Leu-Gly, 0.1 muM) also significantly reduced the damaging effect of H2O2: the number of p53+ cells decreased by 73.5%, the area of cell nuclei returned to normal, and generation of superoxide anion-radical decreased by 18.4%. Hydrogen Peroxide 146-150 tumor protein p53 Homo sapiens 166-169 34447990-6 2021 Thus, the results suggest a critical role for mitochondrially-generated H2O2 in the regulation of p53 expression, which in turn modulates XP-C sensitivity to agents that cause mitochondrial stress. Hydrogen Peroxide 72-76 tumor protein p53 Homo sapiens 98-101 34144191-4 2021 However, H2O2 also induces signaling through stress-activated kinases (SAPK, e.g., JNK and p38MAPK) that can activate p53. Hydrogen Peroxide 9-13 tumor protein p53 Homo sapiens 118-121 34099041-5 2021 Further, we found that SPD significantly reduced basal or hydrogen peroxide (H2O2)-induced up-regulated protein expression of the aging markers, cyclin dependent kinase inhibitor 2A (p16/CDKN2A) and tumor protein 53 (p53). Hydrogen Peroxide 58-75 tumor protein p53 Homo sapiens 199-215 34187948-0 2021 Luteolin inhibits H2O2-induced cellular senescence via modulation of SIRT1 and p53. Hydrogen Peroxide 18-22 tumor protein p53 Homo sapiens 79-82 34099041-5 2021 Further, we found that SPD significantly reduced basal or hydrogen peroxide (H2O2)-induced up-regulated protein expression of the aging markers, cyclin dependent kinase inhibitor 2A (p16/CDKN2A) and tumor protein 53 (p53). Hydrogen Peroxide 58-75 tumor protein p53 Homo sapiens 217-220 34099041-5 2021 Further, we found that SPD significantly reduced basal or hydrogen peroxide (H2O2)-induced up-regulated protein expression of the aging markers, cyclin dependent kinase inhibitor 2A (p16/CDKN2A) and tumor protein 53 (p53). Hydrogen Peroxide 77-81 tumor protein p53 Homo sapiens 199-215 34099041-5 2021 Further, we found that SPD significantly reduced basal or hydrogen peroxide (H2O2)-induced up-regulated protein expression of the aging markers, cyclin dependent kinase inhibitor 2A (p16/CDKN2A) and tumor protein 53 (p53). Hydrogen Peroxide 77-81 tumor protein p53 Homo sapiens 217-220 34155600-12 2021 Moreover, the H2O2 treatment group showed significantly downregulated expression of SIRT1 (P < 0.01) but significantly upregulated expressions of p53 and Caspase3 (P < 0.01) compared to the control group. Hydrogen Peroxide 14-18 tumor protein p53 Homo sapiens 146-149 34155600-14 2021 This study suggests that SPPA inhibits H2O2-induced human KGN cell apoptosis through antioxidation, and the SIRT1/p53 signal pathway mediates the antioxidation. Hydrogen Peroxide 39-43 tumor protein p53 Homo sapiens 114-117 35436464-6 2022 Furthermore, mutated LCL displayed lower levels of phospho-Chk1 and phospho-Chk2 following hydrogen peroxide-induced oxidative stress, indicating a poorly effective DNA damage checkpoint, as well as reduced basal levels of p53. Hydrogen Peroxide 91-108 tumor protein p53 Homo sapiens 223-226 35592280-10 2022 Moreover, CUR/CDP treatment at 20 and 40 mug/ml inhibited H2O2-induced increase in phosphorylated-p53 and caspase-3 expression, indicating that CUR/CDP suppressed cell apoptosis to alleviate liver injury. Hydrogen Peroxide 58-62 tumor protein p53 Homo sapiens 98-101 35546176-5 2022 The levels of cellular senescence-associated p53 and p21 significantly increased in H2O2-induced senescent House Ear Institute-Organ of Corti 1 (HEI-OC1) cells and cochlear explants. Hydrogen Peroxide 84-88 tumor protein p53 Homo sapiens 45-48 35418568-8 2022 Furthermore, downregulation of REST by H2O2 treatment induced apoptosis in the House Ear Institute Organ of Corti 1 cell, through the upregulation of p53. Hydrogen Peroxide 39-43 tumor protein p53 Homo sapiens 150-153 35204316-10 2022 Furthermore, 300 microM H2O2 affected genes related to the p53 pathway and cell cycle. Hydrogen Peroxide 24-28 tumor protein p53 Homo sapiens 59-62 33297931-13 2020 Further investigation showed that p53 protein levels had been increased during ROCK1-mediated apoptosis in response to H2O2. Hydrogen Peroxide 119-123 tumor protein p53 Homo sapiens 34-37 35204748-6 2022 Moreover, phosphorylation of the apoptotic p53 protein was reduced while the activity of the AKT/mTOR signaling pathway was increased, which was further enhanced by oxidative stress (H2O2) in granulocytes and erythroleukemic K562 cells. Hydrogen Peroxide 183-187 tumor protein p53 Homo sapiens 43-46 35069864-11 2022 PCA also reduced the gene expression of Cox-2, iNOS, p53 and p16 induced by H2O2. Hydrogen Peroxide 76-80 tumor protein p53 Homo sapiens 53-56 35069864-13 2022 Furthermore, PCA suppressed H2O2-induced changes in the protein expression of p16, p53 and collagen-2. Hydrogen Peroxide 28-32 tumor protein p53 Homo sapiens 83-86 35042627-0 2022 The involvement of ERK1/2 and p38 MAPK in the premature senescence of melanocytes induced by H2O2 through a p53-independent p21 pathway. Hydrogen Peroxide 93-97 tumor protein p53 Homo sapiens 108-111 35042627-9 2022 H2O2 treatment tended to induce premature senescence in melanocytes through a p53-independent p21 pathway. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 78-81 35042627-15 2022 CONCLUSION: H2O2 increases ROS levels, which activates the ERK1/2 and p38 MAPK pathways to induce the premature senescence of melanocytes through p21 via a p53-independent pathway and consequently disrupts melanosome transfer. Hydrogen Peroxide 12-16 tumor protein p53 Homo sapiens 156-159 35090313-8 2022 MP treatment alleviated the H2O2-induced increases in ROS levels, inhibited apoptosis, relieved cell cycle arrest, and downregulated cleaved caspase 3 and P53 protein expression. Hydrogen Peroxide 28-32 tumor protein p53 Homo sapiens 155-158 33576462-7 2021 Through downregulating SIRT1 and activating p53 signaling, miR-138-5p induced apoptosis in H2O2-induced AC-16 and HCM cells. Hydrogen Peroxide 91-95 tumor protein p53 Homo sapiens 44-47 33505358-5 2020 We found that p53 was upregulated during cellular senescence and osteogenic differentiation of MSCs respectively induced by H2O2 and BMP9. Hydrogen Peroxide 124-128 tumor protein p53 Homo sapiens 14-17 33934760-8 2021 Then, p53-antigen was immobilized on the nanocomposite and used in an indirect immunoassay with horseradish peroxidase (HRP)-conjugated secondary antibody and H2O2 as the substrate, following the typical Michaelis-Menten kinetics. Hydrogen Peroxide 159-163 tumor protein p53 Homo sapiens 6-9 32679123-5 2020 The treatment of nifedipine dose-dependently suppressed H2O2-induced senescence by reducing Ca2+ entry, autophagy impairment and mTOR signaling, and this suppression was found to be related to senescence-associated beta-galactosidase (SA-beta-gal) activity and the expressions of senescence marker protein 30 (SMP30), p53, and p21. Hydrogen Peroxide 56-60 tumor protein p53 Homo sapiens 318-321 32780285-9 2020 Eventually, the relative proteins expression levels of p53, cleaved caspase-9/3, cytochrome c, Fas-L, Fas, FADD and caspase-8 were substantially up-regulated in H2O2-triggered HepG2 cells, and Bax/Bcl-2 ratio and the relative protein expression levels of PARP were dramatically down-regulated. Hydrogen Peroxide 161-165 tumor protein p53 Homo sapiens 55-58 32901866-4 2020 Co-treatment with the TOPK inhibitor, OTS514, in combination with H2O2 increased p53 acetylation and its expression, whereas it decreased Sirtuin 1 (SIRT1) expression, contributing to the promotion of apoptosis. Hydrogen Peroxide 66-70 tumor protein p53 Homo sapiens 81-84 32901866-6 2020 Furthermore, the p53 inhibitor, Pifithrin-mu, diminished the augmentation in poly(ADP-ribose) polymerase (PARP) cleavage induced by OTS514 plus H2O2, while the Mdm2 antagonist, Nutlin 3, increased PARP cleavage. Hydrogen Peroxide 144-148 tumor protein p53 Homo sapiens 17-20 32901866-7 2020 Moreover, OTS514 further decreased the SIRT1 transcriptional activity decreased by H2O2, but promoted the H2O2-induced p53 or p21 transcriptional activity. Hydrogen Peroxide 106-110 tumor protein p53 Homo sapiens 119-122 32901866-9 2020 Taken together, the findings of the present study demonstrate that TOPK inhibition promotes p53-mediated granulosa cell apoptosis through SIRT1 downregulation in response to H2O2. Hydrogen Peroxide 174-178 tumor protein p53 Homo sapiens 92-95 32901866-10 2020 Therefore, it can be concluded that TOPK suppresses H2O2-induced apoptosis through the modulation of the p53/SIRT1 axis, suggesting a potential role of TOPK in the regulation of human granulosa cell apoptosis, leading to the promotion of abnormal follicular development. Hydrogen Peroxide 52-56 tumor protein p53 Homo sapiens 105-108 33195700-10 2020 This study highlights that miR-122 overexpression may restore H2O2-induced HUVEC injury by regulating the expression of p53. Hydrogen Peroxide 62-66 tumor protein p53 Homo sapiens 120-123 33046085-13 2020 CsA also attenuated the activation of p53, decreased the expression of Bax and cleavage of PARP, and increased the expression of Bcl-2 and pro-caspase-3 in the JEG-3 treated with H2O2. Hydrogen Peroxide 179-183 tumor protein p53 Homo sapiens 38-41 32402583-9 2020 Additionally, an intracellular calcium rise and subsequent mitochondrial membrane potential collapse, P53 phosphorylation, reduced BcL-2/Bax ratio in the mitochondrial membrane, release cytochrome c from mitochondria, leading to the subsequent activation of caspase 3 activation by H2O2 were all markedly suppressed in the presence of luteolin. Hydrogen Peroxide 282-286 tumor protein p53 Homo sapiens 102-105 32801360-5 2020 Then, autophagy improves intracellular H2O2 via promoting p53-mediated depletion of GSH and cysteine and downregulation of xCT. Hydrogen Peroxide 39-43 tumor protein p53 Homo sapiens 58-61 31908576-5 2020 In cultured VSMCs, hydrogen peroxide (H2O2) dose-dependently induced senescence, which was associated with increased numbers of senescence-associated beta-galactosidase-positive cells, decreased expression of SMP30, and activation of p53-p21 and p16 pathways. Hydrogen Peroxide 19-36 tumor protein p53 Homo sapiens 234-237 31894323-13 2020 Knockdown of p53 significantly decreased the expression levels of miR-335-5p in JEG-3 cells and in H2O2-treated cells. Hydrogen Peroxide 99-103 tumor protein p53 Homo sapiens 13-16 31092438-2 2019 The aim of this study was to investigate the relationship between p53 and IGF1 signaling pathway in young, senescent and H2O2-treated cells. Hydrogen Peroxide 121-125 tumor protein p53 Homo sapiens 66-69 31598361-4 2019 In addition, hydrogen peroxide-induced p53 activation and BH3 interacting-domain death agonist (BID) expression were higher in CsA-treated cells than those in non-treated cells, whereas hydrogen peroxide-induced activation of mitogen-activated protein kinases including p38, c-Jun N-terminal kinase, and extracellular signal-regulated kinase and activation of protein kinase B were not significantly altered by treatment with CsA. Hydrogen Peroxide 13-30 tumor protein p53 Homo sapiens 39-42 31598361-4 2019 In addition, hydrogen peroxide-induced p53 activation and BH3 interacting-domain death agonist (BID) expression were higher in CsA-treated cells than those in non-treated cells, whereas hydrogen peroxide-induced activation of mitogen-activated protein kinases including p38, c-Jun N-terminal kinase, and extracellular signal-regulated kinase and activation of protein kinase B were not significantly altered by treatment with CsA. Hydrogen Peroxide 186-203 tumor protein p53 Homo sapiens 39-42 31598361-8 2019 Taken together, these data suggest that CsA can aggravate hydrogen peroxide-induced cell death through p53 activation, BID expression, and ROS production. Hydrogen Peroxide 58-75 tumor protein p53 Homo sapiens 103-106 31109646-7 2019 Furthermore, H2O2 triggers p53 activation and promotes p21 expression, indicating a role for the p53/p21 signaling pathway in oxidative stress-induced senescence in BCSCs. Hydrogen Peroxide 13-17 tumor protein p53 Homo sapiens 27-30 31109646-7 2019 Furthermore, H2O2 triggers p53 activation and promotes p21 expression, indicating a role for the p53/p21 signaling pathway in oxidative stress-induced senescence in BCSCs. Hydrogen Peroxide 13-17 tumor protein p53 Homo sapiens 97-100 31092438-6 2019 The gene expression levels of PI3K, TP53 and catalase (CAT) in senescent and H2O2-treated cells were increased compared to young cells. Hydrogen Peroxide 77-81 tumor protein p53 Homo sapiens 36-40 30303965-4 2018 Biological methods demonstrated the oxidative damage of P19 neurons and showed that quercetin improved neuronal survival by preventing H2O2-induced p53 and Bcl-2 down-regulation and modulated Akt and ERK1/2 signalling pathways. Hydrogen Peroxide 135-139 tumor protein p53 Homo sapiens 148-151 30988280-6 2019 H2O2 activated AMPK, which then phosphorylated SIRT1 and inhibited its deacetylation activity toward p53 in A549 cells or FOXO1 in NCI-H1299 cells. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 101-104 30456590-5 2019 H2O2 treated cells showed characteristic senescence-associated features including increased cell size, senescence-associated beta-galactosidase activity (SA-beta-gal), development of senescence-associated secretory phenotype (SASP), activation of reactive oxygen species (ROS) and pathways, DNA damage as well as induction of cell cycle inhibitors (p53/p21WAF1/p16INK4a). Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 349-352 30697266-12 2019 Co-treatment with captopril and the AKT inhibitor MK-2206 reduced the H2O2-induced P53 and ICAM-1 protein expression (p < 0.05). Hydrogen Peroxide 70-74 tumor protein p53 Homo sapiens 83-86 29803744-5 2018 SIRT1 protein level in ADSCs was increased by the treatment with H2O2, meanwhile, H2O2 activated p53-depended apoptosis in high concentration. Hydrogen Peroxide 65-69 tumor protein p53 Homo sapiens 97-100 29803744-5 2018 SIRT1 protein level in ADSCs was increased by the treatment with H2O2, meanwhile, H2O2 activated p53-depended apoptosis in high concentration. Hydrogen Peroxide 82-86 tumor protein p53 Homo sapiens 97-100 28860061-6 2018 In addition, porphyran significantly affected the p53-p21 pathways in H2O2-treated WI-38 cells. Hydrogen Peroxide 70-74 tumor protein p53 Homo sapiens 50-53 30280787-11 2018 RESULTS: Growing levels of DUSP, aging proteins P53 and P16, with inhibition of TLR4 signal pathway were found in the H2O2-induced aging model of myeloma cell line RPMI8226. Hydrogen Peroxide 118-122 tumor protein p53 Homo sapiens 48-51 30027149-7 2018 In the Calligonum extract+H2O2 group, apoptosis, as well as expression of apoptotic genes (Bax and P53), was significantly lower than the group treated with H2O2 alone. Hydrogen Peroxide 26-30 tumor protein p53 Homo sapiens 99-102 29600625-1 2018 This study aimed to investigate the effect of notoginsenoside R1 in delaying H2O2-induced vascular endothelial cell senescence through microRNA-34a/SIRT1/p53 signal pathway. Hydrogen Peroxide 77-81 tumor protein p53 Homo sapiens 154-157 29600625-14 2018 The possible mechanism is that notoginsenoside R1 can delay the senescence process of vascular endothelial cells induced by H2O2 by regulating microRNA-34a/SIRT1/p53 signal pathway. Hydrogen Peroxide 124-128 tumor protein p53 Homo sapiens 162-165 29702192-8 2018 Moreover, PAB depleted intracellular GSH via p53-mediated xCT pathway, which further exacerbated accumulation of H2O2 and lipid peroxides. Hydrogen Peroxide 113-117 tumor protein p53 Homo sapiens 45-48 29920999-6 2018 Interestingly, p53 pathway proteins and mitogen-activated protein kinase (MAPK) signalling pathway components were up-regulated by H2 O2 but exhibited a downward trend in MSCs co-cultured with ADASs. Hydrogen Peroxide 131-136 tumor protein p53 Homo sapiens 15-18 29667777-7 2018 The results showed that iPS-CM significantly reduced H2 O2 -induced ILC apoptosis through down-regulation of autophagic and apoptotic proteins LC3-I/II, Beclin-1, P62, P53 and BAX as well as up-regulation of BCL-2, which could be inhibited by LY294002 (25 mumol/L). Hydrogen Peroxide 53-58 tumor protein p53 Homo sapiens 168-171 29579543-8 2018 Furthermore, the expression levels of p300, Ac-p53, p53, p-p21 and p16 were significantly increased in senescent and H2O2-induced cells compared to young cells. Hydrogen Peroxide 117-121 tumor protein p53 Homo sapiens 47-50 29579543-8 2018 Furthermore, the expression levels of p300, Ac-p53, p53, p-p21 and p16 were significantly increased in senescent and H2O2-induced cells compared to young cells. Hydrogen Peroxide 117-121 tumor protein p53 Homo sapiens 52-55 29472838-11 2018 In all, these results show that cancer imprints an increased resistance to H2O2-induced death in lymphocytes that persists after AD development, and is dependent on both PARP-1 and p53. Hydrogen Peroxide 75-79 tumor protein p53 Homo sapiens 181-184 29367587-8 2018 H2O2 obviously upregulated expressions of H19, miR-675, and NGF, and downregulated let-7a and TP53 levels; however, MT treatment reduced expressions of H19, miR-675, and NGF, and improved let-7a and TP53 levels. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 94-98 29367587-8 2018 H2O2 obviously upregulated expressions of H19, miR-675, and NGF, and downregulated let-7a and TP53 levels; however, MT treatment reduced expressions of H19, miR-675, and NGF, and improved let-7a and TP53 levels. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 199-203 29331104-2 2018 The present study aimed to investigate whether microRNA-34a (miR-34a), which is a downstream target of P53, is involved in H2O2-induced MSC cell death. Hydrogen Peroxide 123-127 tumor protein p53 Homo sapiens 103-106 29233996-5 2017 However, the PEPD-p53 complex is critical for p53 response to stress, as stress signals doxorubicin and H2O2 each must free p53 from PEPD in order to achieve robust p53 activation, which is mediated by reactive oxygen species. Hydrogen Peroxide 104-108 tumor protein p53 Homo sapiens 18-21 29242407-4 2017 By RNA-sequencing, transcriptome changes were analyzed in human ECs exposed to H2O2, highlighting a pivotal role of p53-signaling. Hydrogen Peroxide 79-83 tumor protein p53 Homo sapiens 116-119 29242407-6 2017 Among microRNAs (miRNAs), miR-192-5p was the most induced by H2O2 treatment, in a p53-dependent manner. Hydrogen Peroxide 61-65 tumor protein p53 Homo sapiens 82-85 29242407-9 2017 A central role of the p53-pathway was also confirmed by the analysis of differential exon usage: Upon H2O2 treatment, the expression of p53-dependent 5"-isoforms of MDM2 and PVT1 increased selectively. Hydrogen Peroxide 102-106 tumor protein p53 Homo sapiens 22-25 29242407-9 2017 A central role of the p53-pathway was also confirmed by the analysis of differential exon usage: Upon H2O2 treatment, the expression of p53-dependent 5"-isoforms of MDM2 and PVT1 increased selectively. Hydrogen Peroxide 102-106 tumor protein p53 Homo sapiens 136-139 29233996-5 2017 However, the PEPD-p53 complex is critical for p53 response to stress, as stress signals doxorubicin and H2O2 each must free p53 from PEPD in order to achieve robust p53 activation, which is mediated by reactive oxygen species. Hydrogen Peroxide 104-108 tumor protein p53 Homo sapiens 46-49 29233996-5 2017 However, the PEPD-p53 complex is critical for p53 response to stress, as stress signals doxorubicin and H2O2 each must free p53 from PEPD in order to achieve robust p53 activation, which is mediated by reactive oxygen species. Hydrogen Peroxide 104-108 tumor protein p53 Homo sapiens 46-49 29233996-5 2017 However, the PEPD-p53 complex is critical for p53 response to stress, as stress signals doxorubicin and H2O2 each must free p53 from PEPD in order to achieve robust p53 activation, which is mediated by reactive oxygen species. Hydrogen Peroxide 104-108 tumor protein p53 Homo sapiens 46-49 28193070-4 2016 After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric signal using the system hydroquinone/H2O2 was related to the levels of p53-autoantibodies in the sample. Hydrogen Peroxide 144-148 tumor protein p53 Homo sapiens 178-181 28801242-8 2017 H2O2 contributed to T/B-mediated ERK1/2 activation that mediated p53 phosphorylation at serine 15 (Ser15) and increased p21 expression. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 65-68 28892622-6 2017 As Au-NPFe2O3NC possess high peroxidase-like activity for the oxidation of TMB in the presence of H2O2 [TMB is a common chromogenic substrate for HRP in enzyme-linked immunosorbent assays (ELISAs)], we envisage that our assay could find a wide range of application in developing ELISA-based sensing approaches in the fields of medicine (i.e., detection of other biomarkers the same as p53 autoantibody), biotechnology, and environmental sciences. Hydrogen Peroxide 98-102 tumor protein p53 Homo sapiens 385-388 29051731-14 2017 In addition, deregulations of the p53 pathway seem to contribute to the H2O2-induced death in MCI and AD lymphocytes, which show increased p53 expression. Hydrogen Peroxide 72-76 tumor protein p53 Homo sapiens 34-37 29051731-14 2017 In addition, deregulations of the p53 pathway seem to contribute to the H2O2-induced death in MCI and AD lymphocytes, which show increased p53 expression. Hydrogen Peroxide 72-76 tumor protein p53 Homo sapiens 139-142 28927457-4 2017 Benzoyl peroxide and hydrogen peroxide elicit oxidative stress, which upregulates p53. Hydrogen Peroxide 21-38 tumor protein p53 Homo sapiens 82-85 28440512-0 2017 Astemizole protects against human umbilical vein endothelial cell injury induced by hydrogen peroxide via the p53 signaling pathway. Hydrogen Peroxide 84-101 tumor protein p53 Homo sapiens 110-113 28440512-8 2017 In conclusion, astemizole effectively protected endothelial cells against oxidative stress induced by H2O2, a function that may involve ROS/p53/p21Cip1/Waf1/ p16INK4a signaling pathways. Hydrogen Peroxide 102-106 tumor protein p53 Homo sapiens 140-143 28694916-6 2017 The H2O2-mediated increased expression of the proapoptotic genes, BAX and p53, was attenuated by IAA treatment, while IAA treatment increased antiapoptotic genes, BCL-2 and ATF5 expression. Hydrogen Peroxide 4-8 tumor protein p53 Homo sapiens 74-77 28130753-7 2017 Our data indicated that up-regulation of intracellular ROS induced by H2O2 significantly inhibited proliferation and induced apoptosis accompanying G1 cell cycle arrest and elevated expression of p53. Hydrogen Peroxide 70-74 tumor protein p53 Homo sapiens 196-199 27090187-2 2016 AEF effectively antagonized the senescence-associated beta-galactosidase staining and upregulation of p53 and p21(Cip1/WAF1) induced by UVB or H2O2 treatment in HS68 cells. Hydrogen Peroxide 143-147 tumor protein p53 Homo sapiens 102-105 27498709-6 2016 The H2O2-exposed hMSCs showed cellular senescence with significantly increased protein levels of acetyl-p53 and p21 in comparison with the untreated hMSCs, and these effects were prevented by pre-treatment with EGCG. Hydrogen Peroxide 4-8 tumor protein p53 Homo sapiens 104-107 27599894-8 2016 H2O2-induced ROS production increased the levels of phosphorylated-p38 mitogen activated protein kinase, c-Jun N-terminal kinase, ataxia telangiectasia mutated and p53; these increases were inhibited by pretreatment with C. setidens. Hydrogen Peroxide 0-4 tumor protein p53 Homo sapiens 164-167