PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24967183-8 2014 Under oxidative damage condition, 2h of pretreatment with 10-100 ng/mL EGF can mostly inhibit 50% lethal dose of 0.08 mmol/L H2O2-induced cell damage. Hydrogen Peroxide 125-129 epidermal growth factor Homo sapiens 71-74 31148064-0 2019 Imaging of Intracellular Hydrogen Peroxide Production with HyPer upon Stimulation of HeLa Cells with EGF. Hydrogen Peroxide 25-42 epidermal growth factor Homo sapiens 101-104 26807851-5 2016 Importantly, NBCD enabled the visualization of epidermal growth factor (EGF)-stimulated H2O2 generation inside the cells. Hydrogen Peroxide 88-92 epidermal growth factor Homo sapiens 47-70 26807851-5 2016 Importantly, NBCD enabled the visualization of epidermal growth factor (EGF)-stimulated H2O2 generation inside the cells. Hydrogen Peroxide 88-92 epidermal growth factor Homo sapiens 72-75 26846883-6 2016 Further assessment of expression characteristics and signaling pathways revealed the activation of three major H2O2-dependent pathways, EGF, FOXO1, and IKKalpha. Hydrogen Peroxide 111-115 epidermal growth factor Homo sapiens 136-139 21254838-0 2011 Assessment of redox changes to hydrogen peroxide-sensitive proteins during EGF signaling. Hydrogen Peroxide 31-48 epidermal growth factor Homo sapiens 75-78 22894498-0 2012 Real-time imaging elucidates the role of H2O2 in regulating kinetics of epidermal growth factor-induced and Src-mediated tyrosine phosphorylation signaling. Hydrogen Peroxide 41-45 epidermal growth factor Homo sapiens 72-95 21606925-0 2011 Epidermal growth factor protects the apical junctional complexes from hydrogen peroxide in bile duct epithelium. Hydrogen Peroxide 70-87 epidermal growth factor Homo sapiens 0-23 21606925-11 2011 Pretreatment of cell monolayers with EGF ameliorated hydrogen peroxide-induced tight junction disruption and barrier dysfunction. Hydrogen Peroxide 53-70 epidermal growth factor Homo sapiens 37-40 21606925-13 2011 Hydrogen peroxide increased tyrosine phosphorylation of ZO-1, claudin-3, E-cadherin and beta-catenin, and pretreatment of cells with EGF attenuated tyrosine phosphorylation of these proteins. Hydrogen Peroxide 0-17 epidermal growth factor Homo sapiens 133-136 21606925-15 2011 EGF prevents hydrogen peroxide-induced tight junction disruption by a PLCgamma and PKC-dependent mechanism. Hydrogen Peroxide 13-30 epidermal growth factor Homo sapiens 0-3 17573555-5 2007 We hypothesised that exposure to hydrogen peroxide, a potent generator of ROS, would induce apoptosis in term placental villous explants and that this could be reduced by treatment with EGF. Hydrogen Peroxide 33-50 epidermal growth factor Homo sapiens 186-189 20610539-4 2010 Using 5-(and-6)-chloromethyl-2",7"-dichlorodihydrofluorescein diacetate acetyl ester staining, we found that intracellular hydrogen peroxide (H(2)O(2)) production was increased in EGF-treated cells and could be inhibited by treatment with an EGFR inhibitor, AG1478, or an H(2)O(2) scavenger, polyethylene glycol (PEG)-catalase. Hydrogen Peroxide 123-140 epidermal growth factor Homo sapiens 180-183 20610539-4 2010 Using 5-(and-6)-chloromethyl-2",7"-dichlorodihydrofluorescein diacetate acetyl ester staining, we found that intracellular hydrogen peroxide (H(2)O(2)) production was increased in EGF-treated cells and could be inhibited by treatment with an EGFR inhibitor, AG1478, or an H(2)O(2) scavenger, polyethylene glycol (PEG)-catalase. Hydrogen Peroxide 142-150 epidermal growth factor Homo sapiens 180-183 20961289-4 2011 EGF (epidermal growth factor) potentiated H2O2-induced tight junction disruption in under-differentiated cell monolayers, which was attenuated by the MEK [MAPK (mitogen-activated protein kinase)/ERK kinase] inhibitor U0126. Hydrogen Peroxide 42-46 epidermal growth factor Homo sapiens 0-3 20961289-4 2011 EGF (epidermal growth factor) potentiated H2O2-induced tight junction disruption in under-differentiated cell monolayers, which was attenuated by the MEK [MAPK (mitogen-activated protein kinase)/ERK kinase] inhibitor U0126. Hydrogen Peroxide 42-46 epidermal growth factor Homo sapiens 5-28 20961289-5 2011 In contrast, EGF prevented H2O2-induced disruption of tight junctions in differentiated cell monolayers, which was also attenuated by U0126. Hydrogen Peroxide 27-31 epidermal growth factor Homo sapiens 13-16 20961289-8 2011 EGF prevented both H2O2-induced association of PP2A (protein phosphatase 2A), and loss of association of PKCzeta (protein kinase Czeta), with occludin by an ERK-dependent mechanism in differentiated cell monolayers, but not in under-differentiated cell monolayers. Hydrogen Peroxide 19-23 epidermal growth factor Homo sapiens 0-3 20610539-0 2010 Hydrogen peroxide mediates EGF-induced down-regulation of E-cadherin expression via p38 MAPK and snail in human ovarian cancer cells. Hydrogen Peroxide 0-17 epidermal growth factor Homo sapiens 27-30 17573555-11 2007 Proliferation of cytotrophoblasts within villous explants was significantly reduced following exposure to 1,000 microM hydrogen peroxide, this was restored to control levels by simultaneous treatment with 10 or 100 ng/ml EGF. Hydrogen Peroxide 119-136 epidermal growth factor Homo sapiens 221-224 15670807-0 2005 EGF suppresses hydrogen peroxide induced Ca2+ influx by inhibiting L-type channel activity in cultured human corneal endothelial cells. Hydrogen Peroxide 15-32 epidermal growth factor Homo sapiens 0-3 15368538-0 2005 Effect of EGF on H2O2-induced inhibition of alpha-MG uptake in renal proximal tubule cells: involvement of MAPK and AA release. Hydrogen Peroxide 17-21 epidermal growth factor Homo sapiens 10-13 15368538-6 2005 When PTCs were exposed to 100 microM H2O2 and 50 ng/ml EGF simultaneously, a further increase in the phosphorylation of p44/42 MAPK, of [3H]-AA release, and of prostaglandin E2 (PGE2) production was elicited as compared with the effects of each individual agonist alone. Hydrogen Peroxide 37-41 epidermal growth factor Homo sapiens 55-58 15368538-8 2005 In conclusion, these results are consistent with the hypothesis that under conditions of oxidative stress, the H2O2-induced inhibition of alpha-MG uptake in the renal proximal tubule is mediated through a modulation of the EGF signaling pathway, promoting further phosphorylation of p44/42 MAPK, activation of PLA2. Hydrogen Peroxide 111-115 epidermal growth factor Homo sapiens 223-226 15670807-3 2005 Whereas pathophysiological concentrations of H2O2 (10 mM) induced irreversible large increases in [Ca2+]i, lower concentrations (up to 1 mM) had smaller effects, which were further reduced by exposure to either 5 microM nifedipine or EGF (10 ng ml(-1)). Hydrogen Peroxide 45-49 epidermal growth factor Homo sapiens 234-237 15670807-4 2005 EGF had a larger protective effect against H2O2-induced rises in [Ca2+]i than nifedipine. Hydrogen Peroxide 43-47 epidermal growth factor Homo sapiens 0-3 15670807-7 2005 Taken together, H2O2 induces rises in [Ca2+]i that occur through increases in Ca2+ permeation along plasma membrane pathways that include L-type Ca2+ channels as well as other EGF-sensitive pathways. Hydrogen Peroxide 16-20 epidermal growth factor Homo sapiens 176-179 15670807-8 2005 As EGF overcomes H2O2-induced rises in [Ca2+]i, its presence during eye bank storage could improve the outcome of corneal transplant surgery. Hydrogen Peroxide 17-21 epidermal growth factor Homo sapiens 3-6 14633709-10 2003 Taken together, these data indicate that BPQs, through the generation of hydrogen peroxide, activate the EGFR in MCF-10A cells, leading to increased cell number under EGF-deficient conditions. Hydrogen Peroxide 73-90 epidermal growth factor Homo sapiens 105-108 14750954-3 2003 RESULTS: Here, we show that by using A431 cells as a model system, expression of p52shc, or cell stimulation with EGF or H2O2 leads to phosphorylation of EGFR on Tyr 845 that is located to the activation segment of the catalytic domain. Hydrogen Peroxide 121-125 epidermal growth factor Homo sapiens 154-158 12063263-2 2002 In contrast, we have shown previously that exposure to oxidative stress in the form of hydrogen peroxide (H(2)O(2)) activated the EGF receptor but that the levels of activated receptors did not decline, which resulted in prolonged receptor signaling. Hydrogen Peroxide 87-104 epidermal growth factor Homo sapiens 130-133 35152831-9 2022 However, it is interesting that EGF-mediated intracellular signaling was significantly down-regulated in the H2O2-induced senescent HUVEC, and physiological concentration of UA could at least partially restore the EGF-mediated signaling. Hydrogen Peroxide 109-113 epidermal growth factor Homo sapiens 32-35 10505733-5 1999 Coadministration of epidermal growth factor (EGF) for 24 hr reduced the H2O2 and XO + X-induced inhibition of DNA synthesis; this effect of EGF was not observed up to 8 hr. Hydrogen Peroxide 72-76 epidermal growth factor Homo sapiens 45-48 10348664-8 1999 In contrast to the results with carbachol, H2O2 potentiated EGF-induced tyrosine phosphorylation. Hydrogen Peroxide 43-47 epidermal growth factor Homo sapiens 60-63 11156945-1 2001 Recently, we demonstrated that hydrogen peroxide (H2O2) inhibits the internalization of the epidermal growth factor (EGF) receptor and the EGF-induced mono-ubiquitination of EGF receptor pathway substrate clone #15 (Eps15) in fibroblasts. Hydrogen Peroxide 31-48 epidermal growth factor Homo sapiens 117-120 11156945-1 2001 Recently, we demonstrated that hydrogen peroxide (H2O2) inhibits the internalization of the epidermal growth factor (EGF) receptor and the EGF-induced mono-ubiquitination of EGF receptor pathway substrate clone #15 (Eps15) in fibroblasts. Hydrogen Peroxide 50-54 epidermal growth factor Homo sapiens 117-120 11156945-2 2001 In addition, it was suggested that EGF receptor internalization might be inhibited by H2O2 by inhibition of ubiquitination of proteins involved in endocytosis. Hydrogen Peroxide 86-90 epidermal growth factor Homo sapiens 35-38 11156945-3 2001 Here, we show that H2O2 also inhibits the poly-ubiquitination of the EGF receptor in fibroblasts. Hydrogen Peroxide 19-23 epidermal growth factor Homo sapiens 69-72 10037455-5 1999 Administration of EGF delayed H2O2 and XO + X-induced changes in TER, dilution potential, and [3H]mannitol flux. Hydrogen Peroxide 30-34 epidermal growth factor Homo sapiens 18-21 35152831-9 2022 However, it is interesting that EGF-mediated intracellular signaling was significantly down-regulated in the H2O2-induced senescent HUVEC, and physiological concentration of UA could at least partially restore the EGF-mediated signaling. Hydrogen Peroxide 109-113 epidermal growth factor Homo sapiens 214-217 3045813-5 1988 After treatment with H2O2, [Ahx21]-hEGF was clearly separated from methionine-oxidized hEGF by one-step reverse-phase HPLC. Hydrogen Peroxide 21-25 epidermal growth factor Homo sapiens 35-39 3045813-5 1988 After treatment with H2O2, [Ahx21]-hEGF was clearly separated from methionine-oxidized hEGF by one-step reverse-phase HPLC. Hydrogen Peroxide 21-25 epidermal growth factor Homo sapiens 87-91 30476538-1 2019 Binding of epidermal growth factor (EGF) to its cell surface receptor induces production of H2O2, which serves as an intracellular messenger. Hydrogen Peroxide 92-96 epidermal growth factor Homo sapiens 11-34 30926755-4 2019 To validate the signaling role of H2O2, we showed that epidermal growth factor induces a transient increase in intracellular H2O2 levels, and the essential cysteine residue of protein-tyrosine phosphatases is a target for specific and reversible oxidation by the H2O2 produced in such cells. Hydrogen Peroxide 34-38 epidermal growth factor Homo sapiens 55-78 30926755-4 2019 To validate the signaling role of H2O2, we showed that epidermal growth factor induces a transient increase in intracellular H2O2 levels, and the essential cysteine residue of protein-tyrosine phosphatases is a target for specific and reversible oxidation by the H2O2 produced in such cells. Hydrogen Peroxide 125-129 epidermal growth factor Homo sapiens 55-78 30926755-4 2019 To validate the signaling role of H2O2, we showed that epidermal growth factor induces a transient increase in intracellular H2O2 levels, and the essential cysteine residue of protein-tyrosine phosphatases is a target for specific and reversible oxidation by the H2O2 produced in such cells. Hydrogen Peroxide 125-129 epidermal growth factor Homo sapiens 55-78 30476538-0 2019 Inactivation of the PtdIns(4)P phosphatase Sac1 at the Golgi by H2O2 produced via Ca2+-dependent Duox in EGF-stimulated cells. Hydrogen Peroxide 64-68 epidermal growth factor Homo sapiens 105-108 30476538-1 2019 Binding of epidermal growth factor (EGF) to its cell surface receptor induces production of H2O2, which serves as an intracellular messenger. Hydrogen Peroxide 92-96 epidermal growth factor Homo sapiens 36-39 30476538-4 2019 EGF induced a transient increase in Golgi PtdIns(4)P as well as a transient oxidation of Sac1 in a manner dependent on elevation of the intracellular Ca2+ concentration and on H2O2. Hydrogen Peroxide 176-180 epidermal growth factor Homo sapiens 0-3 30476538-7 2019 Expression of a Golgi-targeted H2O2 probe revealed transient EGF-induced H2O2 production at this organelle. Hydrogen Peroxide 31-35 epidermal growth factor Homo sapiens 61-64 30476538-7 2019 Expression of a Golgi-targeted H2O2 probe revealed transient EGF-induced H2O2 production at this organelle. Hydrogen Peroxide 73-77 epidermal growth factor Homo sapiens 61-64 30476538-8 2019 Our findings have thus uncovered a previously unrecognized EGF signaling pathway that links intracellular Ca2+ mobilization to events at the Golgi including Duox activation, H2O2 production, Sac1 oxidation, and PtdIns(4)P accumulation. Hydrogen Peroxide 174-178 epidermal growth factor Homo sapiens 59-62