PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 34238157-8 2022 Human RPE cells overexpressing PEDF and/or GM-CSF or pre-treated with recombinant proteins presented significantly increased glutathione levels post-H2O2 incubation than non-transfected/untreated controls. Hydrogen Peroxide 149-153 serpin family F member 1 Homo sapiens 31-35 34238157-11 2022 PEDF and/or GM-CSF reduced H2O2-mediated oxidative stress damage in RPE cells and ROC offering an encouraging technique to re-establish a cell-protective environment to halt age-related retinal degeneration. Hydrogen Peroxide 27-31 serpin family F member 1 Homo sapiens 0-4 30431098-7 2019 The results of cell viability assays demonstrated that the numbers of apoptotic cells were increased following treatment with H2O2 in a dose-dependent manner; however, this effect was reversed following treatment with PEDF. Hydrogen Peroxide 126-130 serpin family F member 1 Homo sapiens 218-222 33369607-5 2020 To confirm the functionality and the potency of the PEDF and GM-CSF secreted by the transfected RPE cells, we have developed an in vitro assay to quantify the reduction of H2O2-induced oxidative stress on RPE cells in culture. Hydrogen Peroxide 172-176 serpin family F member 1 Homo sapiens 52-56 30431098-10 2019 Labeling of the UCP2 detector in the confocal images demonstrated decreased UCP2 protein staining in the retinal pigment epithelium (RPE) cells and RPE layers following H2O2 injury; however, this effect was inhibited following treatment with PEDF. Hydrogen Peroxide 169-173 serpin family F member 1 Homo sapiens 242-246 24762585-0 2014 Anti-inflammatory effects of a synthetic peptide derived from pigment epithelium-derived factor on H2O2-induced corneal injury in vitro. Hydrogen Peroxide 99-103 serpin family F member 1 Homo sapiens 62-95 25064413-7 2014 RESULT(S): We found that granulosa cell viability and the level of PEDF mRNA were both significantly reduced, in a dose-dependent manner, after exposure to H2O2. Hydrogen Peroxide 156-160 serpin family F member 1 Homo sapiens 67-71 24762585-3 2014 This study was to investigate the anti-inflammatory effects of PEDF-34 on H2O2-induced corneal injury in vitro. Hydrogen Peroxide 74-78 serpin family F member 1 Homo sapiens 63-67 24762585-10 2014 Western blotting analysis showed that the VEGF and ICAM-1 levels in the HCFs and HUVECs stimulated by H2O2 were significantly higher than those in the normal conditions, which were decreased dramatically in those treated with PEDF-34-NPs. Hydrogen Peroxide 102-106 serpin family F member 1 Homo sapiens 226-230 21946416-5 2012 The data show cortical cultures release PEDF and that H2O2 treatment causes cell death, increases activated caspase 3 levels and decreases release of PEDF. Hydrogen Peroxide 54-58 serpin family F member 1 Homo sapiens 150-154 21946416-8 2012 Treating cortical cultures with PEDF 24 h before H2O2 exposure mitigates oxidant-induced decreases in neuronal survival, Bcl-2 expression, and phosphorylation of ERK1/2 and also reduces elevated caspase 3 level and activity. Hydrogen Peroxide 49-53 serpin family F member 1 Homo sapiens 32-36 21946416-10 2012 However, only inhibition of ERK reduced the ability of PEDF to protect neurons from H2O2-induced Bcl-2 decrease and neuronal death. Hydrogen Peroxide 84-88 serpin family F member 1 Homo sapiens 55-59 16425980-5 2005 Various types of immunochemically distinct AGEs, which were prepared in vitro by incubating bovine serum albumin with glucose, glyceraldehyde or glycolaldehyde, significantly decreased endothelial mRNA levels of PEDF Furthermore, H2O2 dose-dependently suppressed PEDF gene expression in ECs. Hydrogen Peroxide 230-234 serpin family F member 1 Homo sapiens 212-216 16504212-3 2006 In the present study, the influence of PEDF in hydrogen peroxide (H(2)O(2))-induced RPE cell death was investigated using nontransformed human RPE cell line (ARPE-19). Hydrogen Peroxide 47-64 serpin family F member 1 Homo sapiens 39-43 16504212-3 2006 In the present study, the influence of PEDF in hydrogen peroxide (H(2)O(2))-induced RPE cell death was investigated using nontransformed human RPE cell line (ARPE-19). Hydrogen Peroxide 66-74 serpin family F member 1 Homo sapiens 39-43 16504212-5 2006 The MTT cell viability assay showed that PEDF rescued ARPE-19 from H(2)O(2)-induced cell death in a dose- and time-dependent manner. Hydrogen Peroxide 67-75 serpin family F member 1 Homo sapiens 41-45 17593873-0 2007 Hydrogen peroxide stimulates pigment epithelium-derived factor gene and protein expression in the human hepatocyte cell line OUMS-29. Hydrogen Peroxide 0-17 serpin family F member 1 Homo sapiens 29-62 17593873-2 2007 Since oxidative stress has been postulated to play an important role in the pathogenesis of vascular injury in the metabolic syndrome, this study investigated the effects of hydrogen peroxide (H2O2) on PEDF in the immortalized human hepatocyte cell line OUMS-29. Hydrogen Peroxide 174-191 serpin family F member 1 Homo sapiens 202-206 17593873-2 2007 Since oxidative stress has been postulated to play an important role in the pathogenesis of vascular injury in the metabolic syndrome, this study investigated the effects of hydrogen peroxide (H2O2) on PEDF in the immortalized human hepatocyte cell line OUMS-29. Hydrogen Peroxide 193-197 serpin family F member 1 Homo sapiens 202-206 17593873-4 2007 H2O2 upregulated PEDF mRNA levels and increased PEDF protein production in OUMS-29 cells in time- and dose-dependent manners. Hydrogen Peroxide 0-4 serpin family F member 1 Homo sapiens 17-21 17593873-4 2007 H2O2 upregulated PEDF mRNA levels and increased PEDF protein production in OUMS-29 cells in time- and dose-dependent manners. Hydrogen Peroxide 0-4 serpin family F member 1 Homo sapiens 48-52 17593873-5 2007 The anti-oxidant N-acetylcysteine significantly blocked H2O2-induced PEDF overexpression in OUMS-29 cells. Hydrogen Peroxide 56-60 serpin family F member 1 Homo sapiens 69-73 17593873-7 2007 H2O2-induced PEDF overproduction in the liver may act as a negative feedback system against vascular damage in the metabolic syndrome. Hydrogen Peroxide 0-4 serpin family F member 1 Homo sapiens 13-17 16483542-7 2006 PEDF can also prevent H(2)O(2)-induced stress kinase p38/27-kDa heat shock protein signaling which is known to mediate actin rearrangement. Hydrogen Peroxide 22-30 serpin family F member 1 Homo sapiens 0-4 15797260-7 2005 An increased ratio of bax to bcl-2 mRNA level with subsequent activation of caspase-3 was observed in high-glucose- or H2O2-exposed pericytes, which was also completely prevented by PEDF. Hydrogen Peroxide 119-123 serpin family F member 1 Homo sapiens 182-186 15797260-9 2005 Further, PEDF was found to completely inhibit high-glucose- or H2O2-induced increase in a mRNA ratio of angiopoietin-2 to angiopoietin-1 and up-regulation of VEGF mRNA levels in pericytes. Hydrogen Peroxide 63-67 serpin family F member 1 Homo sapiens 9-13 15797260-10 2005 PEDF mRNA levels themselves were down-regulated in high-glucose- or H2O2-exposed pericytes. Hydrogen Peroxide 68-72 serpin family F member 1 Homo sapiens 0-4 15797260-11 2005 These results demonstrate that PEDF protects against high-glucose- or H2O2-induced pericyte apoptosis and dysfunction through its anti-oxidative properties via GPx induction. Hydrogen Peroxide 70-74 serpin family F member 1 Homo sapiens 31-35 11748590-8 2001 Conversely, while elevated levels of PEDF transcript and protein were seen in differentiated RPE cells, compared to non-differentiated cells, a marked decrease at both PEDF mRNA and protein levels was seen after treatment with H2O2. Hydrogen Peroxide 227-231 serpin family F member 1 Homo sapiens 37-41 11748590-8 2001 Conversely, while elevated levels of PEDF transcript and protein were seen in differentiated RPE cells, compared to non-differentiated cells, a marked decrease at both PEDF mRNA and protein levels was seen after treatment with H2O2. Hydrogen Peroxide 227-231 serpin family F member 1 Homo sapiens 168-172