PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25405767-6 2014 Exposure of islets lacking functional NLRP3 or caspase-1 to H2O2, rotenone or thapsigargin induced similar cell death as in wild-type islets. Hydrogen Peroxide 60-64 NLR family, pyrin domain containing 3 Mus musculus 38-43 27308893-6 2017 Furthermore, priming of the NLRP3 inflammasome was sensitive to the exogenous ROS levels induced by H2O2 or rotenone. Hydrogen Peroxide 100-104 NLR family, pyrin domain containing 3 Mus musculus 28-33 34904823-3 2022 Hence, the present study aimed to investigate the role of lipopolysaccharide (LPS) and hydrogen peroxide (H2O2) in activating NLRP3 inflammasome-driven neurodegeneration and elucidated the neuroprotective role of perillyl alcohol (PA) in in vitro and in vivo models of Parkinson"s disease (PD). Hydrogen Peroxide 87-104 NLR family, pyrin domain containing 3 Mus musculus 126-131 24140862-7 2014 In vivo, scavenging of O2(-) by Tempol and removal of H2O2 by catalase substantially inhibited NLRP3 inflammasome formation and activation in glomeruli of hHcys mice as shown by reduced colocalization of NLRP3 with ASC or caspase-1 and inhibition of caspase-1 activation and IL-1beta production. Hydrogen Peroxide 54-58 NLR family, pyrin domain containing 3 Mus musculus 95-100 24140862-9 2014 In conclusion, endogenously produced O2(-) and H2O2 primarily contribute to NLRP3 inflammasome formation and activation in mouse glomeruli resulting in glomerular injury or consequent sclerosis during hHcys. Hydrogen Peroxide 47-51 NLR family, pyrin domain containing 3 Mus musculus 76-81 34904823-3 2022 Hence, the present study aimed to investigate the role of lipopolysaccharide (LPS) and hydrogen peroxide (H2O2) in activating NLRP3 inflammasome-driven neurodegeneration and elucidated the neuroprotective role of perillyl alcohol (PA) in in vitro and in vivo models of Parkinson"s disease (PD). Hydrogen Peroxide 106-110 NLR family, pyrin domain containing 3 Mus musculus 126-131 34904823-4 2022 Initial priming of microglial cells with LPS following treatment with H2O2 induced NF-kappaB translocation to the nucleus with a robust generation of free radicals that act as signal 2 in augmenting NLRP3 inflammasome assembly and its downstream targets. Hydrogen Peroxide 70-74 NLR family, pyrin domain containing 3 Mus musculus 199-204 32194416-7 2020 Whereas, the inhibitory effect of BBR on ROS, TXNIP expression, NLRP3 inflammasome activation and pyroptosis could be reversed by H2O2 in AML12 cells. Hydrogen Peroxide 130-134 NLR family, pyrin domain containing 3 Mus musculus 64-69 34229586-3 2021 The mice and cellular models for IVD degeneration were established by using puncture method and H2O2 exposure, respectively, and we evidenced that NLRP3-mediated cell pyroptosis, apoptosis and inflammatory responses occurred during IVD degeneration progression in vitro and in vivo. Hydrogen Peroxide 96-100 NLR family, pyrin domain containing 3 Mus musculus 147-152 35091064-7 2022 The mechanistic effect of HMGB2 was studied in the 661w cell line treated with H2O2, showing that exogenous recombinant HMGB2 protein reduced the expressions of the antioxidant protein nuclear erythroid factor 2-related factor 2 (Nrf2) and its downstream target heme oxygenase-1 (HO-1), and induced NF-kappaB/NLRP3 signaling pathway. Hydrogen Peroxide 79-83 NLR family, pyrin domain containing 3 Mus musculus 309-314 34271086-9 2021 Though the intracellular levels of pyroptosis-related cytokine caspase-1, cleaved caspase-1, NLRP3, IL-18, IL-1beta were upregulated both in MI and H2O2 stimulation, knockout of TN-C resisted such injury and alleviated cardiac pyroptosis, which further decreased IL-6, TNF-alpha, MCP-1 expression. Hydrogen Peroxide 148-152 NLR family, pyrin domain containing 3 Mus musculus 93-98 32470468-9 2020 Additionally, both conditioned medium from H9c2 cardiomyocytes exposed to hydrogen peroxide (H9c2-H2O2-CM) and combination of mtDNA and ATP (mtDNA-ATP) increased the expression of NLRP3 and cleaved caspase-1 (p10) as well as intracellular ROS production in RAW264.7 macrophages, which were abrogated by Met treatment. Hydrogen Peroxide 74-91 NLR family, pyrin domain containing 3 Mus musculus 180-185 32470468-9 2020 Additionally, both conditioned medium from H9c2 cardiomyocytes exposed to hydrogen peroxide (H9c2-H2O2-CM) and combination of mtDNA and ATP (mtDNA-ATP) increased the expression of NLRP3 and cleaved caspase-1 (p10) as well as intracellular ROS production in RAW264.7 macrophages, which were abrogated by Met treatment. Hydrogen Peroxide 98-102 NLR family, pyrin domain containing 3 Mus musculus 180-185 31060676-3 2019 Results After treatment with H2O2,the expression of caspase-1 protein and NLRP3 mRNA in BV2 cells was increased,and IL-1beta protein in BV2 cells was significantly increased after treatment with GdCl3(P=0.0036).After treatment with CA-074Me,the doses of NLRP3 mRNA(P=0.037),caspase-1(P=0.021),and IL-1beta(P= 0.036)were significantly reduced.Cells in the H2O2 group and H2O2+GdCl3 group grew more slowly.The expressions of CTSB mRNA and TRPML1 mRNA,or CTSB and TRPML1 proteins in BV2 cells in the treatment group with 200 mumol/L of H2O2 concentration were similar.H2O2-induced CTSB protein expression was inhibited after silencing TRPML1 gene.The changes of other cathepsins were not affected for the different concentration of H2O2.In the BV2 cells treated with TRPML1 gene silencing,the expression of CTSB protein was significantly reduced and the difference was statistically significant(P=0.021)between the H2O2 +siRNA treatment group and the H2O2 treatment group.<b>Conclusion</b> CTSB regulates the activation of NLRP3 inflammasome in the oxidative stress model of microglia cells,probably mediated by calcium channel protein TRPML1. Hydrogen Peroxide 29-33 NLR family, pyrin domain containing 3 Mus musculus 74-79 31137980-10 2019 Inhibition of hydrogen peroxide production from superoxide anions in the gp91phox-/- status prevented the increased TEWL and decreased skin hydration level noted with degradation of NLRP3 and caspase-1. Hydrogen Peroxide 14-31 NLR family, pyrin domain containing 3 Mus musculus 182-187 31060676-3 2019 Results After treatment with H2O2,the expression of caspase-1 protein and NLRP3 mRNA in BV2 cells was increased,and IL-1beta protein in BV2 cells was significantly increased after treatment with GdCl3(P=0.0036).After treatment with CA-074Me,the doses of NLRP3 mRNA(P=0.037),caspase-1(P=0.021),and IL-1beta(P= 0.036)were significantly reduced.Cells in the H2O2 group and H2O2+GdCl3 group grew more slowly.The expressions of CTSB mRNA and TRPML1 mRNA,or CTSB and TRPML1 proteins in BV2 cells in the treatment group with 200 mumol/L of H2O2 concentration were similar.H2O2-induced CTSB protein expression was inhibited after silencing TRPML1 gene.The changes of other cathepsins were not affected for the different concentration of H2O2.In the BV2 cells treated with TRPML1 gene silencing,the expression of CTSB protein was significantly reduced and the difference was statistically significant(P=0.021)between the H2O2 +siRNA treatment group and the H2O2 treatment group.<b>Conclusion</b> CTSB regulates the activation of NLRP3 inflammasome in the oxidative stress model of microglia cells,probably mediated by calcium channel protein TRPML1. Hydrogen Peroxide 29-33 NLR family, pyrin domain containing 3 Mus musculus 254-259 31060676-3 2019 Results After treatment with H2O2,the expression of caspase-1 protein and NLRP3 mRNA in BV2 cells was increased,and IL-1beta protein in BV2 cells was significantly increased after treatment with GdCl3(P=0.0036).After treatment with CA-074Me,the doses of NLRP3 mRNA(P=0.037),caspase-1(P=0.021),and IL-1beta(P= 0.036)were significantly reduced.Cells in the H2O2 group and H2O2+GdCl3 group grew more slowly.The expressions of CTSB mRNA and TRPML1 mRNA,or CTSB and TRPML1 proteins in BV2 cells in the treatment group with 200 mumol/L of H2O2 concentration were similar.H2O2-induced CTSB protein expression was inhibited after silencing TRPML1 gene.The changes of other cathepsins were not affected for the different concentration of H2O2.In the BV2 cells treated with TRPML1 gene silencing,the expression of CTSB protein was significantly reduced and the difference was statistically significant(P=0.021)between the H2O2 +siRNA treatment group and the H2O2 treatment group.<b>Conclusion</b> CTSB regulates the activation of NLRP3 inflammasome in the oxidative stress model of microglia cells,probably mediated by calcium channel protein TRPML1. Hydrogen Peroxide 29-33 NLR family, pyrin domain containing 3 Mus musculus 254-259 29393339-3 2018 The objective of the present study was to investigate whether NALP3 inflammasome activation is involved in H2O2-mediated collagen synthesis, in addition to examining the possible cell signaling mechanisms underlying this effect. Hydrogen Peroxide 107-111 NLR family, pyrin domain containing 3 Mus musculus 62-67 29859240-4 2018 We reported here that H2S attenuated hydrogen peroxide (H2O2)-induced NLRP3 inflammasome activation, which led to caspase-1 activation and IL-1beta production in macrophages. Hydrogen Peroxide 37-54 NLR family, pyrin domain containing 3 Mus musculus 70-75 29859240-4 2018 We reported here that H2S attenuated hydrogen peroxide (H2O2)-induced NLRP3 inflammasome activation, which led to caspase-1 activation and IL-1beta production in macrophages. Hydrogen Peroxide 56-60 NLR family, pyrin domain containing 3 Mus musculus 70-75 29393339-5 2018 H2O2-exposed fibroblasts exhibited activated NALP3 inflammasomes via increased NALP3, apoptosis-associated Speck-like protein and caspase-1 expression and the secretion of interleukin-1beta. Hydrogen Peroxide 0-4 NLR family, pyrin domain containing 3 Mus musculus 45-50 29393339-5 2018 H2O2-exposed fibroblasts exhibited activated NALP3 inflammasomes via increased NALP3, apoptosis-associated Speck-like protein and caspase-1 expression and the secretion of interleukin-1beta. Hydrogen Peroxide 0-4 NLR family, pyrin domain containing 3 Mus musculus 79-84 29393339-9 2018 Taken together, these findings indicate that the NALP3 inflammasome is involved in H2O2-induced type I collagen synthesis, which is mediated by the NF-kappaB signaling pathway. Hydrogen Peroxide 83-87 NLR family, pyrin domain containing 3 Mus musculus 49-54 29156799-11 2017 In vitro, AA concentration-dependently inhibited LPS/H2O2-induced NLRP3 inflammaosome activation in KCs and RAW264.7 cells. Hydrogen Peroxide 53-57 NLR family, pyrin domain containing 3 Mus musculus 66-71 28752965-12 2017 in vitroexperiment, H2O2 increased NALP3 (P<0.05), ASC (P<0.01), caspase-1 (P<0.05) expressions and IL-1beta releasing (P<0.05)and promoted the expressions of collagen-1 and alpha-SMA in both gene and protein levels (P<0.05) in NIH-3T3. Hydrogen Peroxide 20-24 NLR family, pyrin domain containing 3 Mus musculus 35-40 28752965-13 2017 NALP3 activation was partly inhibited in H2O2+SF group (P<0.05). Hydrogen Peroxide 41-45 NLR family, pyrin domain containing 3 Mus musculus 0-5