PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 17177332-5 2006 RESULT: SDS-PAGE and Western blot confirmed that gp120 N and gp120 N/IFN-gamma fusion proteins were successfully expressed. Sodium Dodecyl Sulfate 8-11 interferon gamma Homo sapiens 61-78 18589818-5 2008 The preliminary verification indicates that HBscFv-IFNgamma has the bioactivity of HBscFv and IFNgamma by SDS-PAGE, Western blotting and ELISA. Sodium Dodecyl Sulfate 106-109 interferon gamma Homo sapiens 51-59 18589818-5 2008 The preliminary verification indicates that HBscFv-IFNgamma has the bioactivity of HBscFv and IFNgamma by SDS-PAGE, Western blotting and ELISA. Sodium Dodecyl Sulfate 106-109 interferon gamma Homo sapiens 94-102 15281097-4 2004 SDS-PAGE showed successful conjugation of IFN-gamma with the temperature-sensitive polymer. Sodium Dodecyl Sulfate 0-3 interferon gamma Homo sapiens 42-51 12788634-8 2003 Western blot analysis showed that, in cells treated with interferon gamma, E5 could prevent the breakdown of Ii and block the formation of peptide-loaded, SDS-stable mature MHC class II dimers, correlating with diminished surface MHC class II expression. Sodium Dodecyl Sulfate 155-158 interferon gamma Homo sapiens 57-73 10954204-5 2000 The other difference was an increased interferon-gamma staining of infiltrating mononuclear inflammatory cells in the dermis in the sodium lauryl sulphate group compared with the nickel group. Sodium Dodecyl Sulfate 132-154 interferon gamma Homo sapiens 38-54 12062936-10 2002 For the weakly responding sensitisers DEA and MBT both IFN-gamma and IL-4 cytokine production was only observed after pretreatment of the animals with 10% SDS. Sodium Dodecyl Sulfate 155-158 interferon gamma Homo sapiens 55-64 8783018-2 1996 Sodium dodecyl sulfate-polyacrylamide get electrophoresis (SDS-PAGE) of natural, human interferon-gamma (IFN-gamma) showed two glycosylated variants with apparent molecular masses of 20 and 24 kDa. Sodium Dodecyl Sulfate 0-22 interferon gamma Homo sapiens 87-103 9350283-3 1997 SDS-PAGE analysis of HUVEC glycoproteins, metabolically radiolabelled in the carbohydrate portion, indicated that addition of IFN-gamma produced an altered protein glycosylation. Sodium Dodecyl Sulfate 0-3 interferon gamma Homo sapiens 126-135 8880934-8 1996 SDS PAGE analysis of the crystals demonstrated that both interferon gamma and the receptor were present. Sodium Dodecyl Sulfate 0-3 interferon gamma Homo sapiens 57-73 8783018-2 1996 Sodium dodecyl sulfate-polyacrylamide get electrophoresis (SDS-PAGE) of natural, human interferon-gamma (IFN-gamma) showed two glycosylated variants with apparent molecular masses of 20 and 24 kDa. Sodium Dodecyl Sulfate 0-22 interferon gamma Homo sapiens 105-114 8783018-2 1996 Sodium dodecyl sulfate-polyacrylamide get electrophoresis (SDS-PAGE) of natural, human interferon-gamma (IFN-gamma) showed two glycosylated variants with apparent molecular masses of 20 and 24 kDa. Sodium Dodecyl Sulfate 59-62 interferon gamma Homo sapiens 87-103 8783018-2 1996 Sodium dodecyl sulfate-polyacrylamide get electrophoresis (SDS-PAGE) of natural, human interferon-gamma (IFN-gamma) showed two glycosylated variants with apparent molecular masses of 20 and 24 kDa. Sodium Dodecyl Sulfate 59-62 interferon gamma Homo sapiens 105-114 8016072-3 1994 To understand the relationship between the solution form of IFN-gamma and the moiety that actually binds to the cellular receptor and activates cells, we examined irradiated nonradioactive and 32P-labeled IFN-gamma for its migration in SDS/polyacrylamide gels (to determine its physical integrity), its binding to cells, its reactivity in an ELISA, and its antiviral activity. Sodium Dodecyl Sulfate 236-239 interferon gamma Homo sapiens 60-69 7595201-7 1995 The CHO cell lines, IFN-gamma-treated human peripheral-blood monocytes, and IFN-gamma-treated cells of the human monocytic cell line THP-1 all secreted multiple and identical HuMig species as revealed by SDS-PAGE. Sodium Dodecyl Sulfate 204-207 interferon gamma Homo sapiens 76-85 7748537-0 1995 Interferon-gamma and interleukin-4 production by human T cells recognizing Leishmania donovani antigens separated by SDS-PAGE. Sodium Dodecyl Sulfate 117-120 interferon gamma Homo sapiens 0-16 8016072-3 1994 To understand the relationship between the solution form of IFN-gamma and the moiety that actually binds to the cellular receptor and activates cells, we examined irradiated nonradioactive and 32P-labeled IFN-gamma for its migration in SDS/polyacrylamide gels (to determine its physical integrity), its binding to cells, its reactivity in an ELISA, and its antiviral activity. Sodium Dodecyl Sulfate 236-239 interferon gamma Homo sapiens 205-214 2493219-1 1989 Purified preparations of recombinant human interferon-gamma (rIFN-gamma) with Cys-Tyr-Cys at the N-terminus ([ Cys-Tyr-Cys]IFN-gamma) derived from Escherichia coli gave two closely migrating bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two peaks on reversed-phase high-performance liquid chromatography (rpHPLC). Sodium Dodecyl Sulfate 200-222 interferon gamma Homo sapiens 43-59 1379978-3 1992 PAGE/SDS gel investigations indicated that TNF alpha bound non-covalently while the IFN gamma interaction was covalent in nature. Sodium Dodecyl Sulfate 5-8 interferon gamma Homo sapiens 84-93 1374108-4 1992 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of covalently cross-linked 32P-labeled IFN-gamma and its receptor is consistent with a molecular mass of approximately 99 kD. Sodium Dodecyl Sulfate 0-22 interferon gamma Homo sapiens 117-126 2147181-10 1990 Sodium dodecyl sulfate-polyacrylamide gel analysis of the eluted mixture showed the presence of both interferon gamma and interferon gamma receptor at a ratio of 2:1. Sodium Dodecyl Sulfate 0-22 interferon gamma Homo sapiens 101-147 2493219-1 1989 Purified preparations of recombinant human interferon-gamma (rIFN-gamma) with Cys-Tyr-Cys at the N-terminus ([ Cys-Tyr-Cys]IFN-gamma) derived from Escherichia coli gave two closely migrating bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two peaks on reversed-phase high-performance liquid chromatography (rpHPLC). Sodium Dodecyl Sulfate 259-262 interferon gamma Homo sapiens 43-59 8299680-3 1994 Sodium dodecylsulfate polyacrylamide gel electrophoresis (PAGE) and silver staining of purified N-IFN-gamma displayed three bands with an approximate molecular mass of 66, 62 and 54 kDa. Sodium Dodecyl Sulfate 0-21 interferon gamma Homo sapiens 98-107 8349687-6 1993 IFN gamma also induced dimerization of IFN gamma receptors expressed at the cell surface as detected by chemically cross-linking receptor bound ligand and analyzing cell lysates by SDS-polyacrylamide gel electrophoresis and immunoblotting. Sodium Dodecyl Sulfate 181-184 interferon gamma Homo sapiens 0-9 8349687-6 1993 IFN gamma also induced dimerization of IFN gamma receptors expressed at the cell surface as detected by chemically cross-linking receptor bound ligand and analyzing cell lysates by SDS-polyacrylamide gel electrophoresis and immunoblotting. Sodium Dodecyl Sulfate 181-184 interferon gamma Homo sapiens 39-48 1374108-4 1992 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of covalently cross-linked 32P-labeled IFN-gamma and its receptor is consistent with a molecular mass of approximately 99 kD. Sodium Dodecyl Sulfate 59-62 interferon gamma Homo sapiens 117-126 2466913-2 1988 When [32P]HuIFN-gamma was bound and crosslinked to IFN-gamma the receptor of human cells with a bifunctional crosslinker disuccinimidyl suberate (DSS), a single diffused 32P-labeled band corresponding to the IFN-gamma.receptor complex was visualized by SDS-polyacrylamide gel electrophoresis and autoradiography. Sodium Dodecyl Sulfate 253-256 interferon gamma Homo sapiens 12-21 2466913-2 1988 When [32P]HuIFN-gamma was bound and crosslinked to IFN-gamma the receptor of human cells with a bifunctional crosslinker disuccinimidyl suberate (DSS), a single diffused 32P-labeled band corresponding to the IFN-gamma.receptor complex was visualized by SDS-polyacrylamide gel electrophoresis and autoradiography. Sodium Dodecyl Sulfate 253-256 interferon gamma Homo sapiens 51-60 2934408-8 1985 Monocytes degraded 125I-IFN gamma into trichloroacetic acid-soluble counts at 37 degrees C but not at 4 degrees C, at an approximate rate of 5,000 molecules/cell per h. The receptor was partially characterized by SDS-polyacrylamide gel electrophoresis analysis of purified U937 membranes that had been incubated with 125I-IFN gamma. Sodium Dodecyl Sulfate 213-216 interferon gamma Homo sapiens 24-33 2949008-4 1987 Cross-linking of 125I-rHuIFN-gamma to platelet membrane proteins with the use of a bifunctional agent (DSS) yielded a predominant complex of 100,000 +/- 5,000 daltons on SDS-PAGE autoradiography, which confirms the presence of specific receptors for IFN-gamma. Sodium Dodecyl Sulfate 170-173 interferon gamma Homo sapiens 25-34 3106525-2 1986 One preparation of CHO-derived IFN-gamma showed three bands, with the middle band being a doublet, in a SDS-polyacrylamide gel. Sodium Dodecyl Sulfate 104-107 interferon gamma Homo sapiens 31-40 2944578-6 1986 Cross-linking of membrane-bound 125I-IFN-gamma with disuccinimidyl suberate and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed, in both leukemia and carcinoma cells, three distinct complexes with molecular weights of approximately 70,000, 92,000, and 160,000, suggesting the existence of IFN-gamma receptor subunits. Sodium Dodecyl Sulfate 91-113 interferon gamma Homo sapiens 37-46 3010991-2 1986 After phosphorylation, interferon-gamma was separated from the adenosine phosphates and the kinase and analyzed by SDS-PAGE, reverse phase HPLC, and HPLC peptide mapping. Sodium Dodecyl Sulfate 115-118 interferon gamma Homo sapiens 23-39 2934742-1 1986 When 32P-labeled human recombinant immune interferon gamma (Hu-[32P]IFN-gamma) is crosslinked to human cells with disuccinimidyl suberate, a complex with a molecular size of approximately equal to 117,000 Da was identified by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate 226-248 interferon gamma Homo sapiens 68-77 2934408-8 1985 Monocytes degraded 125I-IFN gamma into trichloroacetic acid-soluble counts at 37 degrees C but not at 4 degrees C, at an approximate rate of 5,000 molecules/cell per h. The receptor was partially characterized by SDS-polyacrylamide gel electrophoresis analysis of purified U937 membranes that had been incubated with 125I-IFN gamma. Sodium Dodecyl Sulfate 213-216 interferon gamma Homo sapiens 322-331 6425412-7 1984 Analysis of IFN-gamma eluted from the column by NaDodSO4/polyacrylamide gel electrophoresis (SDS-PAGE) indicated that both of the known molecular weight subspecies of IFN-gamma (25,000 and 20,000 MW), as well as the presumed dimer of 45,000 MW, were retained by the B1 antibody affinity column. Sodium Dodecyl Sulfate 93-96 interferon gamma Homo sapiens 12-21 2410553-2 1985 Using SDS-polyacrylamide gel electrophoresis, we found that both the monomeric (17 000 to 18 000) and dimeric (34 000 to 35 000) molecular weight forms of IFN-gamma became intensely radioactive. Sodium Dodecyl Sulfate 6-9 interferon gamma Homo sapiens 155-164 6423641-2 1984 35S-IFN-gamma, immunoprecipitated from the crude culture fluid with a monoclonal antibody, was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis into three monomeric forms with molecular weights of 25,000, 20,000, and 15,500, which we designate IFN-gamma I, II, and III, respectively. Sodium Dodecyl Sulfate 107-129 interferon gamma Homo sapiens 4-13 6425412-7 1984 Analysis of IFN-gamma eluted from the column by NaDodSO4/polyacrylamide gel electrophoresis (SDS-PAGE) indicated that both of the known molecular weight subspecies of IFN-gamma (25,000 and 20,000 MW), as well as the presumed dimer of 45,000 MW, were retained by the B1 antibody affinity column. Sodium Dodecyl Sulfate 93-96 interferon gamma Homo sapiens 167-176 6417232-10 1983 In comparison, Escherichia coli-derived recombinant human IFN-gamma separated by SDS-PAGE yielded two major active fractions with m.w. Sodium Dodecyl Sulfate 81-84 interferon gamma Homo sapiens 58-67 6305965-7 1983 125I-labeled IFN-gamma was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis into two bands with molecular weights of 25,000 and 20,000. Sodium Dodecyl Sulfate 39-61 interferon gamma Homo sapiens 13-22 6300774-5 1983 Molecular weight of hIFN-gamma produced by E. coli was estimated to be about 32,000 and 17,000 by gel filtration and SDS-polyacrylamide gel electrophoresis respectively. Sodium Dodecyl Sulfate 117-120 interferon gamma Homo sapiens 20-30 30943978-7 2019 Extensive aerosol studies (aerosol output and droplet analysis, non-invasive and invasive aerosol therapy) were conducted in line with regulatory requirements and characterization of the stability and bioactivity of the IFN-gamma post-nebulization was confirmed using SDS-PAGE and stimulation of Human C-X-C motif chemokine 10 (CXCL 10) also known as IFN-gamma-induced protein 10KDa (IP 10) expression from THP-1 derived macrophages (THP-1 cells). Sodium Dodecyl Sulfate 268-271 interferon gamma Homo sapiens 220-229 6170583-7 1981 IFN produced after stimulation with TPA or mezerein, singly or in combination with phytohemagglutinin, had several properties characteristic of IFN-gamma, e.g., it was largely inactivated by dialysis at pH 2, or after exposure to sodium dodecyl sulfate, whereas it was not neutralized by antibody to IFN-alpha and IFN-beta. Sodium Dodecyl Sulfate 230-252 interferon gamma Homo sapiens 144-153 30943978-12 2019 SDS-PAGE gels indicated that IFN-gamma remains stable after nebulization by both devices and this was confirmed by bioactivity studies using a THP-1 cell model in which an alveolar macrophage response to IFN-gamma was determined. Sodium Dodecyl Sulfate 0-3 interferon gamma Homo sapiens 29-38 30943978-12 2019 SDS-PAGE gels indicated that IFN-gamma remains stable after nebulization by both devices and this was confirmed by bioactivity studies using a THP-1 cell model in which an alveolar macrophage response to IFN-gamma was determined. Sodium Dodecyl Sulfate 0-3 interferon gamma Homo sapiens 204-213