PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3492551-3 1987 The purity of the IL 1 was assessed by fluorography of one- and two-dimensional SDS-polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate 80-83 interleukin 1 alpha Homo sapiens 18-22 2656872-2 1989 Interleukin 1 alpha and beta immunoreactive species were detected by Western blot analysis only when epidermal extracts were obtained in extraction buffers containing dithiothreitol (DTT), sodium dodecyl sulfate (SDS), or 2 mercaptoethanol. Sodium Dodecyl Sulfate 189-211 interleukin 1 alpha Homo sapiens 0-19 2656872-2 1989 Interleukin 1 alpha and beta immunoreactive species were detected by Western blot analysis only when epidermal extracts were obtained in extraction buffers containing dithiothreitol (DTT), sodium dodecyl sulfate (SDS), or 2 mercaptoethanol. Sodium Dodecyl Sulfate 213-216 interleukin 1 alpha Homo sapiens 0-19 2649105-5 1989 Immunoaffinity chromatography followed by Western blotting shows that IL-6 species secreted by IL-1 alpha-induced HUVEC are of molecular mass 23-25, 27-30 and 45 kDa as judged by SDS-PAGE under reducing conditions. Sodium Dodecyl Sulfate 179-182 interleukin 1 alpha Homo sapiens 95-105 2527495-9 1989 The covalently labelled IL-1 receptors in both cell types were sensitive to treatment with endoglycosidase F, which decreased their molecular mass on SDS/PAGE by 12-13 kDa. Sodium Dodecyl Sulfate 150-153 interleukin 1 alpha Homo sapiens 24-28 6336003-4 1984 Both charged species of IL-1, pI 5.1 and 6.8 have a molecular weight of 14,500 as determined by SDS-polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate 96-99 interleukin 1 alpha Homo sapiens 24-28 2581705-3 1985 IL-1 activity was eluted from a single site from PAGE performed in the absence of SDS. Sodium Dodecyl Sulfate 82-85 interleukin 1 alpha Homo sapiens 0-4 3486814-5 1986 This could be demonstrated by incubating the plasma membranes in the presence of IL 1 and (gamma-32P)-ATP, separating the proteins by SDS-PAGE and subsequent autoradiography. Sodium Dodecyl Sulfate 134-137 interleukin 1 alpha Homo sapiens 81-85 19182376-4 2009 We detected the induction of IL-1alpha expression and its secretion into the cell culture medium by treatment with 0.075% SDS for 18 h in LabCyte culture using DNA microarray, quantitative reverse-transcription polymerase chain reaction (RT-PCR) and ELISA. Sodium Dodecyl Sulfate 122-125 interleukin 1 alpha Homo sapiens 29-38 32645342-6 2020 Further, IL-1alpha and MIF levels in EpiDerm, LabCyte, and EpiSkin models stimulated with sodium dodecyl sulfate (SDS) were inversely correlated with cell viability, and were detected even at low SDS concentrations without cell toxicity. Sodium Dodecyl Sulfate 90-112 interleukin 1 alpha Homo sapiens 9-18 32645342-6 2020 Further, IL-1alpha and MIF levels in EpiDerm, LabCyte, and EpiSkin models stimulated with sodium dodecyl sulfate (SDS) were inversely correlated with cell viability, and were detected even at low SDS concentrations without cell toxicity. Sodium Dodecyl Sulfate 114-117 interleukin 1 alpha Homo sapiens 9-18 32645342-6 2020 Further, IL-1alpha and MIF levels in EpiDerm, LabCyte, and EpiSkin models stimulated with sodium dodecyl sulfate (SDS) were inversely correlated with cell viability, and were detected even at low SDS concentrations without cell toxicity. Sodium Dodecyl Sulfate 196-199 interleukin 1 alpha Homo sapiens 9-18 27829611-6 2016 HaCaT keratinocytes treated with SLS had increased levels of intracellular ROS and IL-1alpha secretion. Sodium Dodecyl Sulfate 33-36 interleukin 1 alpha Homo sapiens 83-92 6602068-3 1983 The homogeneous IL 1 retains only a trace of its original biological activity because of the denturing effects of the sodium dodecyl sulfate used in the final step of purification. Sodium Dodecyl Sulfate 118-140 interleukin 1 alpha Homo sapiens 16-20 20564616-10 2010 To confirm paracrine signaling a twofold increase in IL-1 alpha was measured in keratinocyte-conditioned medium after incubation with SDS or Formi, which correlated with fibroblast NF-kappaB activity. Sodium Dodecyl Sulfate 134-137 interleukin 1 alpha Homo sapiens 53-63 19878710-0 2010 A comparative study of leukaemia inhibitory factor and interleukin-1alpha intracellular content in a human keratinocyte cell line after exposure to cosmetic fragrances and sodium dodecyl sulphate. Sodium Dodecyl Sulfate 172-195 interleukin 1 alpha Homo sapiens 55-73 20021188-3 2003 We have shown that the allergens nickel, chromium, isoeugenol, and dinitrofluoro benzene induce the secretion of IL-1 betaat levels that are two- to threefold higher than those of controls and that the nonallergens and irritants sodium dodecyl sulfate, Tween-20, acetic acid, sodium hydroxide, and dimethyl sulfoxide fail to induce such a response. Sodium Dodecyl Sulfate 229-251 interleukin 1 alpha Homo sapiens 113-117 15679580-6 2005 Maximal increase in IL-1alpha secretion occurred within 2 h after exposure to both nickel sulfate and SDS and prior to increased chemokine secretion. Sodium Dodecyl Sulfate 102-105 interleukin 1 alpha Homo sapiens 20-29 16676119-5 2006 Incubation of reconstructed epidermis with taurine inhibited cytotoxic and proinflammatory effects induced by sodium dodecyl sulfate including (i) a decrease in interleukin-1 alpha and prostaglandin E2 release, (ii) stabilization of keratinocyte membrane integrity, and (iii) improvement of keratinocyte viability. Sodium Dodecyl Sulfate 110-132 interleukin 1 alpha Homo sapiens 161-180 10951249-4 2000 3,5-xylenol, chloroxylenol, hexyl-resorcinol, and sodium dodecyl sulfate, but not resorcinol or phenol, induced release of interleukin-1alpha from keratinocytes at cytotoxic concentrations. Sodium Dodecyl Sulfate 50-72 interleukin 1 alpha Homo sapiens 123-141 11737818-17 2001 There was a significant increase in IL-1alpha and a directional increase in IL-8 levels in adult skin sites treated with the irritant, sodium lauryl sulfate, even in the absence of visible skin irritation (erythema). Sodium Dodecyl Sulfate 135-156 interleukin 1 alpha Homo sapiens 36-45 12102660-3 2002 In this study, we have determined the mRNA levels of IL-1alpha in the epidermis after topical application of sodium dodecyl sulphate (SLS) in both a commercially available epidermal kit (EpiDerm) and in excised skin. Sodium Dodecyl Sulfate 109-132 interleukin 1 alpha Homo sapiens 53-62 8463123-7 1993 Furthermore, SDS-PAGE analyses of lysates of the B-cell lines that had been affinity cross-linked with 125I-IL-1 alpha revealed two bands corresponding to IL-1R structures of 60 and 110 kD. Sodium Dodecyl Sulfate 13-16 interleukin 1 alpha Homo sapiens 108-118 1354433-0 1992 Study of immune-associated antigens (IL-1 and ICAM-1) in normal human keratinocytes treated by sodium lauryl sulphate. Sodium Dodecyl Sulfate 95-117 interleukin 1 alpha Homo sapiens 9-41 1330002-3 1992 SDS-PAGE indicated that these biological activities were associated with the adaptive release of tunicate IL1-like (tunicate IL-1) molecules by stimulated hemocytes. Sodium Dodecyl Sulfate 0-3 interleukin 1 alpha Homo sapiens 106-109 1330002-3 1992 SDS-PAGE indicated that these biological activities were associated with the adaptive release of tunicate IL1-like (tunicate IL-1) molecules by stimulated hemocytes. Sodium Dodecyl Sulfate 0-3 interleukin 1 alpha Homo sapiens 125-129 1533322-12 1992 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a band at 85 Kd corresponding to the 68-Kd IL-1 receptor type II (IL-1RtII). Sodium Dodecyl Sulfate 0-22 interleukin 1 alpha Homo sapiens 108-112 1830582-10 1991 After cross-linking of IL-1 to cells followed by specific immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a band at 85 kDa corresponding to the 68-kDa IL-1RtII. Sodium Dodecyl Sulfate 79-101 interleukin 1 alpha Homo sapiens 23-27 2104217-8 1990 The specific binding of biosynthetically labeled IL 1 inhibitor to target cells (EL-4.6.1C10) showed a protein of 26 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Sodium Dodecyl Sulfate 138-160 interleukin 1 alpha Homo sapiens 49-53 2104217-8 1990 The specific binding of biosynthetically labeled IL 1 inhibitor to target cells (EL-4.6.1C10) showed a protein of 26 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Sodium Dodecyl Sulfate 197-200 interleukin 1 alpha Homo sapiens 49-53