PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8260593-3 1993 IL-3 produced by activated T cells was biosynthetically labeled with 35S-methionine and the three major IL-3 glycoforms forms, with M(r) values of 22,000, 28,000 and 36,000, were purified using antibody affinity chromatography and preparative SDS-PAGE. Sodium Dodecyl Sulfate 243-246 interleukin 3 Mus musculus 0-4 12207049-3 2002 The purified NIL-3 activity was a protein with relative molecular weight of 54.5 kDa (SDS-PAGE), which inhibited the growth of IL-3 dependent DA-1 cell growth in a dose dependent manner. Sodium Dodecyl Sulfate 86-89 interleukin 3 Mus musculus 14-18 2937061-6 1986 Two bands of MCGF/TCGF activity were eluted from gels after sodium dodecyl sulfate/PAGE; under nonreducing conditions, the activities corresponded to molecular masses of 20 and 15 kDa, while after reduction, the molecular masses were 21 and 16 kDa. Sodium Dodecyl Sulfate 60-82 interleukin 3 Mus musculus 13-17 2088482-5 1990 Chemically cross-linking of IL-3 to surface molecules of IC2 cells produced three types of complexes with Mr 95, 140, and 200-340 kd in SDS-PAGE, and pre-incubation with anti-Aic-2 IgM at 37 degrees C reduced the intensity of the three bands to the same degree. Sodium Dodecyl Sulfate 136-139 interleukin 3 Mus musculus 28-32 2404777-2 1990 When [125I]mIL-3 was cross-linked to receptor sites on the surfaces of intact B6SUtA1 cells with disuccinimidyl suberate (DSS), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed the existence of two radiolabeled species with molecular weights of 140 (p140) and 70 (p70) kd (after subtraction of [125I]mIL-3). Sodium Dodecyl Sulfate 128-150 interleukin 3 Mus musculus 11-16 2404777-2 1990 When [125I]mIL-3 was cross-linked to receptor sites on the surfaces of intact B6SUtA1 cells with disuccinimidyl suberate (DSS), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed the existence of two radiolabeled species with molecular weights of 140 (p140) and 70 (p70) kd (after subtraction of [125I]mIL-3). Sodium Dodecyl Sulfate 187-190 interleukin 3 Mus musculus 11-16 3262613-2 1988 Interleukin 3 (IL-3) derived from mouse T cells was biosynthetically labeled with either [35S]methionine or [3H]mannose, affinity-purified using various anti-IL-3 antibodies, and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate 191-213 interleukin 3 Mus musculus 0-19 2580015-5 1985 On the other hand, minimum size of IL 3 and IFN-gamma were shown to be about 25,000 and 20,000, respectively, by determining the lymphokine activities contained in the extracts from slices of SDS gels. Sodium Dodecyl Sulfate 192-195 interleukin 3 Mus musculus 35-39 3935881-5 1985 All megakaryocyte colony stimulating activity and interleukin 3 activity was found in the 22-25 Kd fraction from a preparative SDS-polyacrylamide gel using non-reducing conditions, a molecular weight range that coincided with the 28-Kd molecule obtained when reducing conditions were employed. Sodium Dodecyl Sulfate 127-130 interleukin 3 Mus musculus 50-63 6429132-7 1984 When purified PSF was chromatographed on a TSK 3000 SW column under denaturing conditions in 0.1% sodium dodecyl sulfate, the single peak of absorbance at 280 nm coincided with a sharp peak of biological activity. Sodium Dodecyl Sulfate 98-120 interleukin 3 Mus musculus 14-17