PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
22280966-3	2012	In order to enhance the freeze tolerance of yeast cells, we constructed a self-cloning diploid baker"s yeast strain with simultaneous accumulation of proline, by expressing the PRO1-I150T allele, encoding the proline-feedback inhibition-less sensitive gamma-glutamyl kinase, and trehalose, by disrupting the NTH1 gene, encoding neutral trehalase.	Trehalose	279-288	glutamate 5-kinase	Saccharomyces cerevisiae S288C	177-181