PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
19703407-0	2009	Kinetics of the thermal inactivation and aggregate formation of rabbit muscle pyruvate kinase in the presence of trehalose.	Trehalose	113-122	pyruvate kinase PKLR	Oryctolagus cuniculus	78-93	
19703407-2	2009	Because dimethylsulfoxide is known to perturb structure and function of many proteins, we have explored the effect of trehalose on the kinetics of thermal inactivation and stability of pyruvate kinase; this is because trehalose, in contrast to dimethyl sulfoxide, is totally excluded from the hydration shell of proteins.	Trehalose	118-127	pyruvate kinase PKLR	Oryctolagus cuniculus	185-200	
19703407-2	2009	Because dimethylsulfoxide is known to perturb structure and function of many proteins, we have explored the effect of trehalose on the kinetics of thermal inactivation and stability of pyruvate kinase; this is because trehalose, in contrast to dimethyl sulfoxide, is totally excluded from the hydration shell of proteins.	Trehalose	218-227	pyruvate kinase PKLR	Oryctolagus cuniculus	185-200	
19703407-3	2009	The results show that 600 mM trehalose inhibits the activity of pyruvate kinase by about 20% at 25 degrees C, however, trehalose protects pyruvate kinase from thermal inactivation at 60 degrees C, increases the Tm(app) of unfolding by 7.2 degrees C, induces a more compact state, and stabilizes its tetrameric structure.	Trehalose	29-38	pyruvate kinase PKLR	Oryctolagus cuniculus	64-79	
19703407-3	2009	The results show that 600 mM trehalose inhibits the activity of pyruvate kinase by about 20% at 25 degrees C, however, trehalose protects pyruvate kinase from thermal inactivation at 60 degrees C, increases the Tm(app) of unfolding by 7.2 degrees C, induces a more compact state, and stabilizes its tetrameric structure.	Trehalose	119-128	pyruvate kinase PKLR	Oryctolagus cuniculus	64-79	
19703407-3	2009	The results show that 600 mM trehalose inhibits the activity of pyruvate kinase by about 20% at 25 degrees C, however, trehalose protects pyruvate kinase from thermal inactivation at 60 degrees C, increases the Tm(app) of unfolding by 7.2 degrees C, induces a more compact state, and stabilizes its tetrameric structure.	Trehalose	119-128	pyruvate kinase PKLR	Oryctolagus cuniculus	138-153