PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 34723563-7 2021 By the addition of NaCl, Tween 20, and urea, we further confirmed that the secondary conformations of Lyz relied greatly on the interactions between TA and Lyz and dominated the growth rate of the multilayers. Sodium Chloride 19-23 lysozyme Homo sapiens 102-105 34764827-3 2021 In the present work, the influence of the pH value and ionic strengths up to 3000 mM of four technically relevant salts (sodium chloride, sodium sulfate, ammonium chloride, and ammonium sulfate) on the lysozyme adsorption on the mixed-mode resin Toyopearl MX-Trp-650M was studied systematically at 25C. Sodium Chloride 121-136 lysozyme Homo sapiens 202-210 34723563-7 2021 By the addition of NaCl, Tween 20, and urea, we further confirmed that the secondary conformations of Lyz relied greatly on the interactions between TA and Lyz and dominated the growth rate of the multilayers. Sodium Chloride 19-23 lysozyme Homo sapiens 156-159 34342225-4 2021 The experiments reveal that the presence of salts (NaCl or NaI) leads to an opposite ion-specific response for the two proteins: an addition of salt to LZM solutions increases water relaxation rates with respect to the salt-free case, while for BSA solutions, a decrease is observed. Sodium Chloride 51-55 lysozyme Homo sapiens 152-155 34265335-3 2021 Lysozyme aqueous solutions undergo LLPS around 0 C in the presence of NaCl near physiological conditions. Sodium Chloride 71-75 lysozyme Homo sapiens 0-8 34265335-4 2021 Here, it is shown that insertion of small amounts of 4-(2-hydroxyethyl)-1-piperazineethanesulfonate (HEPES, 0.1 M) as a second additive to lysozyme-NaCl-water solutions near physiological ionic strength (0.2 M) is an essential step for triggering conversion of protein-rich droplets into another phase. Sodium Chloride 148-152 lysozyme Homo sapiens 139-147 3782460-4 1986 Lysozyme activity in saliva samples made 0.5 M with respect to NaCl was compared with that in untreated samples with and without centrifugation. Sodium Chloride 63-67 lysozyme Homo sapiens 0-8 3117439-1 1987 After diluting faecal samples with a solution of Brij and saline and subsequently ultrafiltrating the faecal mixtures, lysozyme concentration can be reproducibly measured in the obtained faecal fluids, using a turbidimetric method. Sodium Chloride 58-64 lysozyme Homo sapiens 119-127 34179093-2 2021 The free energy of lysozyme interacting with two kinds of polyanionic excipients, citrate and tripolyphosphate, together with sodium chloride and TRIS-buffer, are analysed in multiple-walker metadynamics simulations to understand why tripolyphosphate causes lysozyme to precipitate but citrate does not. Sodium Chloride 126-141 lysozyme Homo sapiens 258-266 34179093-3 2021 The resulting multiscale decomposition of energy and entropy components for water, sodium chloride, excipients and lysozyme reveals that lysozyme is more stabilised by the interaction of tripolyphosphate with basic residues. Sodium Chloride 83-98 lysozyme Homo sapiens 137-145 6746018-4 1984 However, alpha-mannose bearing liposomes with entrapped lysozyme elicited an immune response similar to that induced by lysozyme in saline. Sodium Chloride 132-138 lysozyme Homo sapiens 120-128 33881613-5 2021 We also demonstrated that taurine attenuates LLPS-dependent cloudiness of lysozyme solution with 0.5 or 1 M NaCl at a critical temperature. Sodium Chloride 108-112 lysozyme Homo sapiens 74-82 30583232-3 2019 The dextran-UCON and the PEG-UCON systems with 75 mM NaCl showed effectiveness in separating 75% and 87% of mono-PEGylated lysozyme from the rest of the lysozyme species in the top and bottom phases, respectively. Sodium Chloride 53-57 lysozyme Homo sapiens 123-131 33561513-2 2021 METHODS: CMC/LYZ-ACP nanogels were developed, and the controlled delivery of ACP from the nanogels was induced by the presence of NaCl. Sodium Chloride 130-134 lysozyme Homo sapiens 13-16 30583232-3 2019 The dextran-UCON and the PEG-UCON systems with 75 mM NaCl showed effectiveness in separating 75% and 87% of mono-PEGylated lysozyme from the rest of the lysozyme species in the top and bottom phases, respectively. Sodium Chloride 53-57 lysozyme Homo sapiens 153-161 23804362-6 2013 With added ovalbumin, the lysozyme solubility decreased linearly at low salt concentration in sodium chloride and increased at high salt concentration in ammonium sulfate. Sodium Chloride 94-109 lysozyme Homo sapiens 26-34 27355857-8 2016 It is found that there is a dominant role of electrostatic forces in the HPTS-LYZ interaction process, because an increase in ionic strength by the addition of NaCl dislodges the fluorophore from the protein pocket to the bulk again. Sodium Chloride 160-164 lysozyme Homo sapiens 78-81 25992483-1 2015 A study of lysozyme adsorption below a behenic acid membrane and at the solid-liquid interface between aqueous lysozyme solution and a silicon wafer in the presence of sodium chloride is presented. Sodium Chloride 168-183 lysozyme Homo sapiens 11-19 25728660-8 2015 The adsorbed lysozyme can be eluted using 20mM phosphate buffer (pH 7.0) containing 1.0M NaCl with a recovery of 96%. Sodium Chloride 89-93 lysozyme Homo sapiens 13-21 25541147-7 2015 Crystallizing conditions could be found for lysozyme from chicken egg white using sodium chloride, for human lysozyme using sodium chloride or ammonium sulfate and glucose isomerase using ammonium sulfate. Sodium Chloride 82-97 lysozyme Homo sapiens 44-52 25541147-7 2015 Crystallizing conditions could be found for lysozyme from chicken egg white using sodium chloride, for human lysozyme using sodium chloride or ammonium sulfate and glucose isomerase using ammonium sulfate. Sodium Chloride 124-139 lysozyme Homo sapiens 109-117 19637151-1 2010 The complexation of lysozyme and sodium (sulfamate carboxylate) isoprene/ethylene oxide (SCIEO) at pH = 7.4 and the release of lysozyme from the complexes in the presence of NaCl were investigated. Sodium Chloride 174-178 lysozyme Homo sapiens 20-28 21940222-4 2011 High desorption of lysozyme from Fe(3)O(4) (PEG+CM-CTS) NPs were achieved using phosphate buffer solution (PBS) (20 mM, pH 5.0, 0.2 M NaCl), PBS (20 mM, pH 5.0, 0.5 M NaCl) and acetic acid (0.2 M, pH 4.0) as eluents. Sodium Chloride 134-138 lysozyme Homo sapiens 19-27 21940222-4 2011 High desorption of lysozyme from Fe(3)O(4) (PEG+CM-CTS) NPs were achieved using phosphate buffer solution (PBS) (20 mM, pH 5.0, 0.2 M NaCl), PBS (20 mM, pH 5.0, 0.5 M NaCl) and acetic acid (0.2 M, pH 4.0) as eluents. Sodium Chloride 167-171 lysozyme Homo sapiens 19-27 20945487-8 2010 Another method of reducing phytic acid concentration was to extract human lysozyme from rice flour at pH 10 with 50 mM NaCl in 50 mM sodium carbonate buffer. Sodium Chloride 119-123 lysozyme Homo sapiens 74-82 20570270-6 2010 In sodium chloride all PEGylated lysozyme derivatives are fully soluble in a concentration range between 0.1 mg protein/ml and 10 mg protein/ml. Sodium Chloride 3-18 lysozyme Homo sapiens 33-41 20570270-8 2010 In both salt solutions, ammonium sulfate and sodium chloride, the highest binding capacity of the resin was found for 5 kDa PEGylated lysozyme. Sodium Chloride 45-60 lysozyme Homo sapiens 134-142 20570270-13 2010 In 4 M sodium chloride a resolution of all PEGylated lysozyme forms was achieved. Sodium Chloride 7-22 lysozyme Homo sapiens 53-61 23201720-5 2013 Here, we examine the general features of pattern formation during sessile droplet evaporation of aqueous lysozyme solutions with varying concentrations of NaCl. Sodium Chloride 155-159 lysozyme Homo sapiens 105-113 22823457-7 2012 A cartoon representation of the frozen lysozyme solution in 0 mol/L NaCl is presented based on the SANS and Monte Carlo results, along with those obtained from other complementary methods. Sodium Chloride 68-72 lysozyme Homo sapiens 39-47 19637151-1 2010 The complexation of lysozyme and sodium (sulfamate carboxylate) isoprene/ethylene oxide (SCIEO) at pH = 7.4 and the release of lysozyme from the complexes in the presence of NaCl were investigated. Sodium Chloride 174-178 lysozyme Homo sapiens 127-135 19637151-6 2010 In the presence of NaCl, lysozyme can be released from the complexes. Sodium Chloride 19-23 lysozyme Homo sapiens 25-33 19880295-5 2010 Addition of sodium chloride could impair the neutralizing ability of positively charged lysozyme on negatively charged membrane via chloride counterion binding. Sodium Chloride 12-27 lysozyme Homo sapiens 88-96 19746957-2 2009 Specifically, we have measured the nine multicomponent diffusion coefficients, D(ij), for the lysozyme-poly(ethylene glycol)-NaCl-water system at pH 4.5 and 25 degrees C using precision Rayleigh interferometry. Sodium Chloride 125-129 lysozyme Homo sapiens 94-102 19664778-5 2009 Using lysozyme, in contrast, we could map out viscosity changes of saline solutions for a variety of different salts, for salt concentrations up to 1M, over a wide range of pH values, and over the temperature range most relevant for biological systems (5-40 degrees C). Sodium Chloride 67-73 lysozyme Homo sapiens 6-14 18788805-2 2008 We report measurements of lysozyme solubility in aqueous solutions as a function of NaCl, KCl, and NH4Cl concentrations at 25 degrees C and pH 4.5. Sodium Chloride 84-88 lysozyme Homo sapiens 26-34 17574261-5 2007 Turbidity experiments with varying pH (3-7) and ionic strength also showed decreased complex formation in mixtures between saliva and lysozyme in solution upon NaCl addition up to 200 mM. Sodium Chloride 160-164 lysozyme Homo sapiens 134-142 18641079-1 2008 Electrophoresis of a mixture of NaCl and CaCl2 in a lysozyme crystal is investigated using nonequilibrium molecular dynamics (MD) simulations. Sodium Chloride 32-36 lysozyme Homo sapiens 52-60 18441020-7 2008 An increase of the electrolyte (NaCl) concentration decreases the solubility of lysozyme in the presence and absence of polyethylene glycol. Sodium Chloride 32-36 lysozyme Homo sapiens 80-88 18763996-1 2008 The phase diagrams for lysozyme are calculated for two different precipitant salts, NaCl and NaSCN, using a potential of mean force that takes into account contributions from ion-dispersion forces [M. Bostrom, J. Phys. Sodium Chloride 84-88 lysozyme Homo sapiens 23-31 18763996-4 2008 Our results are consistent with a recent perturbation theory calculation (referenced above) in that the phase diagram for lysozyme with NaCl is quite different than for lysozyme with NaSCN for the same molar concentration (0.2M) . Sodium Chloride 136-140 lysozyme Homo sapiens 122-130 18763996-5 2008 However, in contrast to the perturbation theory calculation, we find that the lysozyme phase diagram with NaCl has a metastable fluid-fluid coexistence curve and that the metastability gap in the case of NaSCN is much larger than predicted by perturbation theory. Sodium Chloride 106-110 lysozyme Homo sapiens 78-86 18310245-4 2008 Using this nondisruptive fluorescent tracer system, we characterized the effects of sodium chloride and ammonium sulfate concentrations on lysozyme-lysozyme interactions by steady-state and time-resolved fluorescence anisotropy measurements and the introduction of a novel interaction parameter, k(rot). Sodium Chloride 84-99 lysozyme Homo sapiens 139-147 18310245-4 2008 Using this nondisruptive fluorescent tracer system, we characterized the effects of sodium chloride and ammonium sulfate concentrations on lysozyme-lysozyme interactions by steady-state and time-resolved fluorescence anisotropy measurements and the introduction of a novel interaction parameter, k(rot). Sodium Chloride 84-99 lysozyme Homo sapiens 148-156 17156984-6 2007 In TRIS buffered saline, the high pI proteins chymotrypsin and lysozyme showed sustained release lasting over 150 h. Release into 0.15% NaCl led to relatively constant release of lysozyme and chymotrypsin over more than 2000 h; reduction of the releasate volume lengthened the lysozyme release to greater than 8 months. Sodium Chloride 136-140 lysozyme Homo sapiens 63-71 17156984-6 2007 In TRIS buffered saline, the high pI proteins chymotrypsin and lysozyme showed sustained release lasting over 150 h. Release into 0.15% NaCl led to relatively constant release of lysozyme and chymotrypsin over more than 2000 h; reduction of the releasate volume lengthened the lysozyme release to greater than 8 months. Sodium Chloride 136-140 lysozyme Homo sapiens 179-187 17156984-6 2007 In TRIS buffered saline, the high pI proteins chymotrypsin and lysozyme showed sustained release lasting over 150 h. Release into 0.15% NaCl led to relatively constant release of lysozyme and chymotrypsin over more than 2000 h; reduction of the releasate volume lengthened the lysozyme release to greater than 8 months. Sodium Chloride 136-140 lysozyme Homo sapiens 179-187 16471691-4 2006 They are compared to those calculated previously from diffusion data for lysozyme in aqueous NaCl and NH4Cl. Sodium Chloride 93-97 lysozyme Homo sapiens 73-81 16898772-2 2006 The comparison with previous results for the lysozyme-NaCl-water ternary system is used to examine the effect of salt stoichiometry on the transport properties of lysozyme-salt aqueous mixtures. Sodium Chloride 54-58 lysozyme Homo sapiens 45-53 16898772-2 2006 The comparison with previous results for the lysozyme-NaCl-water ternary system is used to examine the effect of salt stoichiometry on the transport properties of lysozyme-salt aqueous mixtures. Sodium Chloride 54-58 lysozyme Homo sapiens 163-171 16906839-5 2006 We found that the transition also occurred at lower lysozyme concentration when NaCl induced an attraction between lysozyme molecules. Sodium Chloride 80-84 lysozyme Homo sapiens 52-60 16906839-5 2006 We found that the transition also occurred at lower lysozyme concentration when NaCl induced an attraction between lysozyme molecules. Sodium Chloride 80-84 lysozyme Homo sapiens 115-123 14566069-2 2003 The crude egg white extract was passed through a cation exchanger Streamline trade mark SP and the bound lysozyme was eluted with 5% ammonium carbonate, pH 9.0, containing 1 M NaCl after elution of avidin. Sodium Chloride 176-180 lysozyme Homo sapiens 105-113 15617820-0 2005 HEW lysozyme salting by high-concentration NaCl solutions followed by titration calorimetry. Sodium Chloride 43-47 lysozyme Homo sapiens 4-12 15617820-1 2005 Concentration dependence of NaCl salting of 0-1.5 mM lysozyme solution in 0.1 M sodium acetate buffer, pH 4.25, was investigated for NaCl concentration varying up to 0.9 M. Calorimetric experiments demonstrated that depending on the salt concentration the estimated number of the binding sites on the lysozyme surface varied in the range of 5 up to 13, and the increase of salt concentration caused the decrease of the number of accessible sites. Sodium Chloride 28-32 lysozyme Homo sapiens 53-61 15617820-2 2005 The small, but significant, local maximum centered at 0.63 M NaCl concentration indicated the specific salting-out of the lysozyme accompanied by binding of approximately 2-3 chloride anions. Sodium Chloride 61-65 lysozyme Homo sapiens 122-130 14967244-5 2004 For a 0.05 M NaCl hen-egg lysozyme solution at pH 7, B22 increases from 2.4 x 10(-4) to 4.7 x 10(-4) ml mol/g2 upon addition of monohydric alcohols and to 5.8 x 10(-4) ml mol/g2 upon addition of glycerol. Sodium Chloride 13-17 lysozyme Homo sapiens 26-34 14606900-0 2003 Lysozyme-lysozyme and lysozyme-salt interactions in the aqueous saline solution: a new square-well potential. Sodium Chloride 64-70 lysozyme Homo sapiens 0-8 14606900-0 2003 Lysozyme-lysozyme and lysozyme-salt interactions in the aqueous saline solution: a new square-well potential. Sodium Chloride 64-70 lysozyme Homo sapiens 22-30 11291029-2 2000 Deuterated lysozyme was dissolved in protonated buffer at pH 2.16 and precipitated with ammonium sulfate, sodium chloride, and potassium thiocyanate. Sodium Chloride 106-121 lysozyme Homo sapiens 11-19 11681581-3 2001 In the presence of 1 M NaCl the transition temperature decreased to about 30 degrees C. In a swollen state the size of pore is large enough to accommodate lysozyme (mol. Sodium Chloride 23-27 lysozyme Homo sapiens 155-163 11551435-1 2001 Liquid-liquid phase-separation data were obtained for aqueous saline solutions of hen egg-white lysozyme at a fixed protein concentration (87 g/l). Sodium Chloride 62-68 lysozyme Homo sapiens 96-104 9795050-5 1998 In vitro release experiments indicated that an initial lysozyme release rate from the microspheres was mainly controlled by ionic interaction between basic amino acid residues in lysozyme and free carboxylate groups in PLGA polymer chain ends, which was probed by incubating the microspheres in a series of media having different NaCl concentrations. Sodium Chloride 330-334 lysozyme Homo sapiens 55-63 9615398-8 1998 The results obtained are in agreement with the hypothesis that lysozyme crystals in NaCl grow by addition of monomeric particles. Sodium Chloride 84-88 lysozyme Homo sapiens 63-71 8889187-3 1996 The flux from 25K MWCO membranes has been measured for lysozyme in pH 4.0 buffered solutions of 1, 3, 4, 5, and 7% NaCl over a wide range of protein concentrations. Sodium Chloride 115-119 lysozyme Homo sapiens 55-63 15299857-3 1997 Static and dynamic light scattering have been employed to investigate the behaviour of lysozyme solutions when varying the concentration of (NH(4))(2)SO(4) and NaCl for screening the repulsive forces between the monomers. Sodium Chloride 160-164 lysozyme Homo sapiens 87-95 15299643-0 1996 Repartitioning of NaCl and protein impurities in lysozyme crystallization. Sodium Chloride 18-22 lysozyme Homo sapiens 49-57 1478067-2 1992 Binding of biotin-heparin to immobilized lactoferrin and lysozyme was optimum at pH 6.0, 100 mM NaCl. Sodium Chloride 96-100 lysozyme Homo sapiens 57-65 1765173-1 1991 The intensity autocorrelation functions of light scattered by lysozyme solutions under pre-crystallization conditions in NaCl-containing media were recorded at scattering angles from 20 degrees to 90 degrees. Sodium Chloride 121-125 lysozyme Homo sapiens 62-70