PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30598505-2 2019 Although alpha4 integrin is known to be a potential target of reactive oxygen species (ROS)-induced cysteine glutathionylation, the physiological significance and underlying regulatory mechanism of this event remain elusive. Reactive Oxygen Species 62-85 immunoglobulin (CD79A) binding protein 1 Mus musculus 9-15 30598505-2 2019 Although alpha4 integrin is known to be a potential target of reactive oxygen species (ROS)-induced cysteine glutathionylation, the physiological significance and underlying regulatory mechanism of this event remain elusive. Reactive Oxygen Species 87-90 immunoglobulin (CD79A) binding protein 1 Mus musculus 9-15 30598505-3 2019 Here, using in vitro and in vivo biochemical and cell biology approaches, we show that physiological ROS-induced glutathionylation of alpha4 integrin in neutrophils increases the binding of neutrophil-associated alpha4 integrin to vascular cell adhesion molecule 1 (VCAM-1) on human endothelial cells. Reactive Oxygen Species 101-104 immunoglobulin (CD79A) binding protein 1 Mus musculus 134-140 30598505-3 2019 Here, using in vitro and in vivo biochemical and cell biology approaches, we show that physiological ROS-induced glutathionylation of alpha4 integrin in neutrophils increases the binding of neutrophil-associated alpha4 integrin to vascular cell adhesion molecule 1 (VCAM-1) on human endothelial cells. Reactive Oxygen Species 101-104 immunoglobulin (CD79A) binding protein 1 Mus musculus 212-218 30598505-7 2019 Taken together, our results establish ROS-elicited glutathionylation and its modulation by Grx1 as pivotal regulatory mechanisms controlling alpha4 integrin affinity and neutrophil mobilization from the bone marrow under physiological conditions. Reactive Oxygen Species 38-41 immunoglobulin (CD79A) binding protein 1 Mus musculus 141-147