PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 23416531-2 2013 The predominant functional species is a non-covalent heterodimer of 33 and 13kDa, termed Xa33/13, which has predicted newly exposed C-terminal lysines that are important for tissue plasminogen activator (tPA)-mediated plasminogen activation to plasmin. Lysine 143-150 chromosome 20 open reading frame 181 Homo sapiens 174-202 7620566-3 1995 Internal Arg/Lys residues that become C-terminal upon proteolysis or zymogen activation, such as in the two-chain form of tissue plasminogen activator, may also be removed from the mature protein. Lysine 13-16 chromosome 20 open reading frame 181 Homo sapiens 122-150 7818846-4 1994 Moreover, excess lysine could inhibit the binding of tPA to the 45-kDa protein in both coincubation and reversibility experiments. Lysine 17-23 chromosome 20 open reading frame 181 Homo sapiens 53-56 2508254-5 1989 Three different treatments of citrated plasma samples (acidification/reneutralization, addition of 5 mM EDTA or of 0.5 M lysine) prior to determination by ELISA all resulted in increased tPA levels. Lysine 121-127 chromosome 20 open reading frame 181 Homo sapiens 187-190 3124875-3 1987 Isolation of TPA was done by using a lysine-sepharose affinity chromatoculumn, then, TPA was measured by a fibrin plate method. Lysine 37-43 chromosome 20 open reading frame 181 Homo sapiens 13-16 3105618-4 1987 Acidification or addition of lysine to plasma is also required for maximum immunoadsorption of plasma tPA antigen on anti-tPA-Ig-sepharose. Lysine 29-35 chromosome 20 open reading frame 181 Homo sapiens 102-105 3105618-4 1987 Acidification or addition of lysine to plasma is also required for maximum immunoadsorption of plasma tPA antigen on anti-tPA-Ig-sepharose. Lysine 29-35 chromosome 20 open reading frame 181 Homo sapiens 122-125 4040396-1 1985 Tissue plasminogen activator produced by a human melanoma cell line (Bowes), was purified from large volumes of supernatant fluid using immunosorbent chromatography on monoclonal antibodies, followed by chromatography on lysine-Sepharose 4B and gel filtration on Sephadex G-150. Lysine 221-228 chromosome 20 open reading frame 181 Homo sapiens 0-28 6541374-0 1984 A sensitive assay for tissue plasminogen activator activity in plasma, using adsorption on lysine-sepharose. Lysine 91-97 chromosome 20 open reading frame 181 Homo sapiens 22-50 1932747-6 1991 Lysine analogues and active or diisopropylfluorophosphate-inactivated u-PA inhibited t-PA binding to monocytes, monocytoid cells, and endothelial cells with similar IC50 (concentration producing 50% inhibition) values, suggesting that the same recognition specificity mediates t-PA binding to all of these cell types. Lysine 0-6 chromosome 20 open reading frame 181 Homo sapiens 85-89 1840295-6 1991 dMM treatment was found to increase both the lysine affinity and catalytic activity of tPA. Lysine 45-51 chromosome 20 open reading frame 181 Homo sapiens 87-90 1840295-8 1991 To evaluate the effects of alterations at site 184 and site 448, the catalytic activity and lysine affinity of type I and type II tPA were monitored individually. Lysine 92-98 chromosome 20 open reading frame 181 Homo sapiens 130-133 1840295-9 1991 In the dMM-treated sample, type I tPA (with sugars at sites 117, 184 and 448) was found to have 2- to 3-fold increased catalytic activity and an affinity for lysine which was greater than that of type I from untreated preparations, but less than that of control type II tPA (containing sugar only at sites 117 and 448). Lysine 158-164 chromosome 20 open reading frame 181 Homo sapiens 34-37 28798096-3 2017 Using a multiwell in vitro assay, S100P is now shown for the first time to exhibit a strong, C-terminal lysine-dependent activation of tissue plasminogen activator (tPA), but not of urokinase-catalysed plasminogen activation. Lysine 104-110 chromosome 20 open reading frame 181 Homo sapiens 135-163 23416531-2 2013 The predominant functional species is a non-covalent heterodimer of 33 and 13kDa, termed Xa33/13, which has predicted newly exposed C-terminal lysines that are important for tissue plasminogen activator (tPA)-mediated plasminogen activation to plasmin. Lysine 143-150 chromosome 20 open reading frame 181 Homo sapiens 204-207 12735467-8 2003 The step-by-step modeling of hypothetical affinity pairs between t-PA and different types of oligo/polymer forms of linear and branched lysine derivatives obtained both by initiated polycondensation and solid-phase peptide synthesis using HPMDC seemed to be possible and a quite useful tool. Lysine 136-142 chromosome 20 open reading frame 181 Homo sapiens 65-69 12926031-1 2003 Studies on the interactions of tissue plasminogen activator (tPA) and plasminogen with polyurethane surfaces containing epsilon-lysine moieties (epsilon-amino group free) are reported. Lysine 128-134 chromosome 20 open reading frame 181 Homo sapiens 31-59 12926031-1 2003 Studies on the interactions of tissue plasminogen activator (tPA) and plasminogen with polyurethane surfaces containing epsilon-lysine moieties (epsilon-amino group free) are reported. Lysine 128-134 chromosome 20 open reading frame 181 Homo sapiens 61-64 21791417-3 2011 Retained tPA effectively increased the lysine binding site-dependent binding of plasminogen on the cell surface and pericellular area; this was abolished by inhibition of enzymatic activity of either tPA or plasmin, which suggests that de novo generation of carboxyl-terminal lysine as a consequence of degradation of surface/pericellular proteins by plasmin is essential. Lysine 39-45 chromosome 20 open reading frame 181 Homo sapiens 9-12 21791417-3 2011 Retained tPA effectively increased the lysine binding site-dependent binding of plasminogen on the cell surface and pericellular area; this was abolished by inhibition of enzymatic activity of either tPA or plasmin, which suggests that de novo generation of carboxyl-terminal lysine as a consequence of degradation of surface/pericellular proteins by plasmin is essential. Lysine 39-45 chromosome 20 open reading frame 181 Homo sapiens 200-203 8857956-8 1996 Competition studies with lysine indicated that the binding was largely kringle-dependent, and when binding of tPA to actin was assessed, it also bound to actin with 70-80% of binding inhibited by lysine. Lysine 25-31 chromosome 20 open reading frame 181 Homo sapiens 110-113 8857956-8 1996 Competition studies with lysine indicated that the binding was largely kringle-dependent, and when binding of tPA to actin was assessed, it also bound to actin with 70-80% of binding inhibited by lysine. Lysine 196-202 chromosome 20 open reading frame 181 Homo sapiens 110-113