PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
7836290-0	1995	Characterization of novel yeast RAD6 (UBC2) ubiquitin-conjugating enzyme mutants constructed by charge-to-alanine scanning mutagenesis.	Alanine	106-113	E2 ubiquitin-conjugating protein RAD6	Saccharomyces cerevisiae S288C	32-36	
7836290-0	1995	Characterization of novel yeast RAD6 (UBC2) ubiquitin-conjugating enzyme mutants constructed by charge-to-alanine scanning mutagenesis.	Alanine	106-113	E2 ubiquitin-conjugating protein RAD6	Saccharomyces cerevisiae S288C	38-42	
7836290-3	1995	We have explored the utility of "charge-to-alanine" scanning mutagenesis to generate novel RAD6 mutants that are enzymatically competent with respect to unfacilitated (E3-independent) ubiquitination but that are nevertheless severely handicapped with respect to several in vivo functions.	Alanine	43-50	E2 ubiquitin-conjugating protein RAD6	Saccharomyces cerevisiae S288C	91-95	
2157209-4	1990	Since the formation of a thioester adduct between E2 and ubiquitin is necessary for E2 activity, the single cysteine residue (Cys-88) present in RAD6 was changed to alanine or valine.	Alanine	165-172	E2 ubiquitin-conjugating protein RAD6	Saccharomyces cerevisiae S288C	145-149	
2157209-6	1990	We show that the rad6 Ala-88 and rad6 Val-88 mutant proteins lack the capacity for thioester formation with ubiquitin and, as a consequence, are totally devoid of any E2 activity.	Alanine	22-25	E2 ubiquitin-conjugating protein RAD6	Saccharomyces cerevisiae S288C	17-21	
2157209-7	1990	The rad6 Ala-88 and rad6 Val-88 mutations confer a defect in DNA repair, mutagenesis, and sporulation equivalent to that in the rad6 null allele.	Alanine	9-12	E2 ubiquitin-conjugating protein RAD6	Saccharomyces cerevisiae S288C	4-8