PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9698365-2 1998 The binding site on the chemokine interleukin-8 (IL-8) for the glycosaminoglycan heparin has been characterized using a systematic series of site-directed mutants of IL-8 in which the basic residues of the protein have been replaced by alanine. Alanine 236-243 C-X-C motif chemokine ligand 8 Homo sapiens 34-47 9698365-2 1998 The binding site on the chemokine interleukin-8 (IL-8) for the glycosaminoglycan heparin has been characterized using a systematic series of site-directed mutants of IL-8 in which the basic residues of the protein have been replaced by alanine. Alanine 236-243 C-X-C motif chemokine ligand 8 Homo sapiens 49-53 8903513-4 1996 We first systematically mutated, in groups of two to four, all the residues in the three intracellular loops of the IL-8 type A receptor to alanine and analyzed the mutant receptors transiently expressed in 293 cells. Alanine 140-147 C-X-C motif chemokine ligand 8 Homo sapiens 116-120 9141135-5 1997 The Glu38-->Cys/Cys50-->Ala IL-8 crystallizes in space group P2(1)2(1)2(1) with cell parameters a = 46.4, b = 49.2, and c = 69.5 A, and has been refined to an R-value of 19.4% for data from 10 to 2 A resolution. Alanine 30-33 C-X-C motif chemokine ligand 8 Homo sapiens 34-38 1918013-1 1991 In order to identify residues required for the binding of interleukin-8 (IL-8) to its receptor, mutants were constructed in which clusters of charged amino acids were systematically replaced with alanine along the entire IL-8 sequence. Alanine 196-203 C-X-C motif chemokine ligand 8 Homo sapiens 58-71 8245475-10 1993 If the ELR sequence of IL-8 was substituted with alanines or if the carboxyl terminus distal to C50 was replaced with the MGSA sequence, a reduction occurred in binding competition. Alanine 49-57 C-X-C motif chemokine ligand 8 Homo sapiens 23-27 8103045-4 1993 To identify this key residue, we systematically mutated to Ala all acidic residues present on the ligand accessible surface of IL-8 receptor type A. Alanine 59-62 C-X-C motif chemokine ligand 8 Homo sapiens 127-131 1918013-1 1991 In order to identify residues required for the binding of interleukin-8 (IL-8) to its receptor, mutants were constructed in which clusters of charged amino acids were systematically replaced with alanine along the entire IL-8 sequence. Alanine 196-203 C-X-C motif chemokine ligand 8 Homo sapiens 73-77 2212672-12 1990 Both forms of IL-8 promoted degranulation of cytochalasin B-treated neutrophils [[ser-IL-8]72 (ED50 greater than 10 nM) was two- to three-fold more potent than [ala-IL-8]77], although in this regard they were less active than FMLP. Alanine 51-54 C-X-C motif chemokine ligand 8 Homo sapiens 14-18 1650387-13 1991 Intradermal injections of [Ala-IL-8]77 or [Ser-IL-8]72 induced dose-dependent PMN accumulation, which also was significantly reduced by i.v. Alanine 27-30 C-X-C motif chemokine ligand 8 Homo sapiens 31-35 2212672-4 1990 The IL-8 isolated from each of these cell types is a mixture of two IL-8 polypeptides, one consisting of 72 amino acids (herein called [ser-IL-8]72) and the other 77 amino acids (an N-terminal extended form herein called [ala-IL-8]77). Alanine 222-225 C-X-C motif chemokine ligand 8 Homo sapiens 4-8 2212672-12 1990 Both forms of IL-8 promoted degranulation of cytochalasin B-treated neutrophils [[ser-IL-8]72 (ED50 greater than 10 nM) was two- to three-fold more potent than [ala-IL-8]77], although in this regard they were less active than FMLP. Alanine 51-54 C-X-C motif chemokine ligand 8 Homo sapiens 86-90 2212672-7 1990 Thrombin was found to efficiently convert [ala-IL-8]77 to [ser-IL-8]72. Alanine 43-46 C-X-C motif chemokine ligand 8 Homo sapiens 47-51 2212672-12 1990 Both forms of IL-8 promoted degranulation of cytochalasin B-treated neutrophils [[ser-IL-8]72 (ED50 greater than 10 nM) was two- to three-fold more potent than [ala-IL-8]77], although in this regard they were less active than FMLP. Alanine 51-54 C-X-C motif chemokine ligand 8 Homo sapiens 86-90 2212672-7 1990 Thrombin was found to efficiently convert [ala-IL-8]77 to [ser-IL-8]72. Alanine 43-46 C-X-C motif chemokine ligand 8 Homo sapiens 63-67 2212672-13 1990 Our data suggest that [ala-IL-8]77 and [ser-IL-8]72 have qualitatively similar and potentially complex biological activities, and that full activation of IL-8 requires cleavage to the [ser-IL-8]72 form. Alanine 23-26 C-X-C motif chemokine ligand 8 Homo sapiens 27-31 2212672-9 1990 In competitive binding assays using 125I[ala-IL-8]77 neutrophils exhibited a twofold preference for [ser-IL-8]72 over [ala-IL-8]77. Alanine 41-44 C-X-C motif chemokine ligand 8 Homo sapiens 45-49 2212672-9 1990 In competitive binding assays using 125I[ala-IL-8]77 neutrophils exhibited a twofold preference for [ser-IL-8]72 over [ala-IL-8]77. Alanine 41-44 C-X-C motif chemokine ligand 8 Homo sapiens 105-109 30016752-2 2018 The aim of the study was to evaluate the influence of PDT with delta-aminolevulinic acid (ALA-PDT) used in sub-lethal dose on the interleukins secretion (IL-6, IL-8 and IL-10) by the residual colon cancer cells (CCC) under hypoxia-like conditions (addition of cobalt chloride- CoCl2). Alanine 90-93 C-X-C motif chemokine ligand 8 Homo sapiens 160-164 2212672-9 1990 In competitive binding assays using 125I[ala-IL-8]77 neutrophils exhibited a twofold preference for [ser-IL-8]72 over [ala-IL-8]77. Alanine 41-44 C-X-C motif chemokine ligand 8 Homo sapiens 105-109 2212672-11 1990 However, [ser-IL-8]72 was approximately 10-fold more potent than [ala-IL-8]77 in these assays (ED50 approximately 0.3 nM for [ser-IL-8]72 vs approximately 3 nM for [ala-IL-8]77. Alanine 66-69 C-X-C motif chemokine ligand 8 Homo sapiens 70-74 2212672-11 1990 However, [ser-IL-8]72 was approximately 10-fold more potent than [ala-IL-8]77 in these assays (ED50 approximately 0.3 nM for [ser-IL-8]72 vs approximately 3 nM for [ala-IL-8]77. Alanine 66-69 C-X-C motif chemokine ligand 8 Homo sapiens 70-74 2212672-11 1990 However, [ser-IL-8]72 was approximately 10-fold more potent than [ala-IL-8]77 in these assays (ED50 approximately 0.3 nM for [ser-IL-8]72 vs approximately 3 nM for [ala-IL-8]77. Alanine 66-69 C-X-C motif chemokine ligand 8 Homo sapiens 70-74 34175752-4 2021 The characteristic peak, related to (dipeptide-H + 2Na)+ species, is observed in the mass spectrum of Phe-Ala and Tyr-Ala dipeptides in the presence of NaF, while the breaking of the peptide bond (OC-NH) occurs for the Phe-Phe in the presence of the aforementioned salts. Alanine 106-109 C-X-C motif chemokine ligand 8 Homo sapiens 152-155 33860562-6 2021 Besides, ALA-PDT could up-regulate the expression of CXCL8 in vivo and in vitro, amplifying the inflammatory response by recruiting T cells, B cells, neutrophils and macrophages. Alanine 9-12 C-X-C motif chemokine ligand 8 Homo sapiens 53-58 33860562-7 2021 We also found that ALA-PDT elevated the expression of CXCL8 via p38 pathway. Alanine 19-22 C-X-C motif chemokine ligand 8 Homo sapiens 54-59 33860562-8 2021 SB203580, a p38 pathway inhibitor, decreased the expression of CXCL8 in sebocytes after ALA-PDT. Alanine 88-91 C-X-C motif chemokine ligand 8 Homo sapiens 63-68 33860562-9 2021 These findings indicate that ALA-PDT amplifies the intense inflammatory response in treatment of acne vulgaris via CXCL8. Alanine 29-32 C-X-C motif chemokine ligand 8 Homo sapiens 115-120 30016752-6 2018 After ALA-PDT we found no change in the IL-6 level secreted by SW480 cells, but decrease of IL-6, IL-10 secretion by SW620 cells, an increase in the IL-8 secreted by both cells lines. Alanine 6-9 C-X-C motif chemokine ligand 8 Homo sapiens 149-153 19232500-3 2009 In this study, we show [ala-IL-8](77) is the predominant circulating isoform of IL-8 in premature neonates but not in term neonates/adults, who have [ser-IL-8](72) as the major isoform. Alanine 24-27 C-X-C motif chemokine ligand 8 Homo sapiens 28-32 29510260-7 2018 CONCLUSIONS: ALA-PDT in sublethal doses might influence colon cancer cell"s progression and invasion by reducing the secretion of IL-6, IL-10 and increasing the IL-8 concentration with higher values in the more malignant cell line. Alanine 13-16 C-X-C motif chemokine ligand 8 Homo sapiens 161-165 19232500-3 2009 In this study, we show [ala-IL-8](77) is the predominant circulating isoform of IL-8 in premature neonates but not in term neonates/adults, who have [ser-IL-8](72) as the major isoform. Alanine 24-27 C-X-C motif chemokine ligand 8 Homo sapiens 80-84 23498057-0 2013 Glutamine and alanine-induced differential expression of intracellular IL-6, IL-8, and TNF-alpha in LPS-stimulated monocytes in human whole-blood. Alanine 14-21 C-X-C motif chemokine ligand 8 Homo sapiens 77-81 17883254-3 2007 The inhibition of H 2O 2-induced IL-8 secretion from Caco-2 cells was observed by pretreatment with Cys, Val, Ile, Leu, Trp, His, Lys, and Ala. Alanine 139-142 C-X-C motif chemokine ligand 8 Homo sapiens 33-37 19232500-3 2009 In this study, we show [ala-IL-8](77) is the predominant circulating isoform of IL-8 in premature neonates but not in term neonates/adults, who have [ser-IL-8](72) as the major isoform. Alanine 24-27 C-X-C motif chemokine ligand 8 Homo sapiens 80-84 19232500-4 2009 This isoform switch from the less potent [ala-IL-8](77) to [ser-IL-8](72) correlates with a maturational increase in the neutrophil chemotactic potency of plasma IL-8. Alanine 42-45 C-X-C motif chemokine ligand 8 Homo sapiens 46-50 19232500-4 2009 This isoform switch from the less potent [ala-IL-8](77) to [ser-IL-8](72) correlates with a maturational increase in the neutrophil chemotactic potency of plasma IL-8. Alanine 42-45 C-X-C motif chemokine ligand 8 Homo sapiens 64-68 19232500-4 2009 This isoform switch from the less potent [ala-IL-8](77) to [ser-IL-8](72) correlates with a maturational increase in the neutrophil chemotactic potency of plasma IL-8. Alanine 42-45 C-X-C motif chemokine ligand 8 Homo sapiens 64-68 15297492-0 2004 Alanine-threonine polymorphism of Helicobacter pylori RpoB is correlated with differential induction of interleukin-8 in MKN45 cells. Alanine 0-7 C-X-C motif chemokine ligand 8 Homo sapiens 104-117 12379337-2 2002 Two main forms of IL-8 exist, one containing 77 amino acids (Ala-IL-8(77)) and a second containing 72 amino acids (Ser-IL-8(72)), which comprise more than 90% of IL-8 protein in cell cultures. Alanine 61-64 C-X-C motif chemokine ligand 8 Homo sapiens 18-22