PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30909598-5 2019 Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. Biotin 71-77 coagulation factor II, thrombin Homo sapiens 116-124 29148722-5 2017 The free end of the customized aptamer15 (apt15), which was attached to the microparticles via streptavidin-biotin linkage to form the PS-apt15 conjugate, was used to capture the model target protein, thrombin (th), by binding at exosite I to form the PS-apt15-th complex. Biotin 108-114 coagulation factor II, thrombin Homo sapiens 201-209 28231553-4 2017 MNPs coated with streptavidin can combine with biotin labeled thrombin aptamers. Biotin 47-53 coagulation factor II, thrombin Homo sapiens 62-70 24508546-2 2014 The silica nanoparticles covered by streptavidin were tagged with a thrombin aptamer (5"-biotin-GGTTGGTGTGGTTGG-3"), which was bound to the surface of silica nanoparticle through the specific interaction between streptavidin and biotin. Biotin 89-95 coagulation factor II, thrombin Homo sapiens 68-76 24296061-2 2014 The biotinylated aptamer of thrombin was immobilized onto an avidin-graphite epoxy composite (AvGEC) electrode surface by affinity interaction between biotin and avidin; electrochemical impedance measurements were performed in a solution containing the redox marker ferrocyanide/ferricyanide. Biotin 4-10 coagulation factor II, thrombin Homo sapiens 28-36 23202336-4 2013 Using ATP and thrombin as inputs, the split/intact aptamers as molecular recognition elements, biotin as a tracer, and SA-HR as a reporter molecule, the electronic logic operations can be easily realized by generating amplified current signals as outputs. Biotin 95-101 coagulation factor II, thrombin Homo sapiens 14-22 23584389-3 2013 The other biotinylated thrombin aptamer (TBA15) was grafted onto streptavidin pretreated SPR gold film through biotin-streptavidin recognition. Biotin 10-16 coagulation factor II, thrombin Homo sapiens 23-31 17689549-7 2007 Using a sandwiched assay scheme followed by an additional signaling step involving biotin-streptavidin chemistry, we have observed the simultaneous binding of the 15- and 29-mer aptamers to thrombin protein at different exosites and have found that one aptamer depletes thrombin"s affinity to the other when they bind together. Biotin 83-89 coagulation factor II, thrombin Homo sapiens 190-198 21393252-3 2011 Here we describe the purification of a phospholipase A(1) (PLA(1)) from thrombin-activated human platelets using sequential chromatographic steps followed by fluorophosphonate (FP)-biotin affinity labeling and proteomics characterization that identified acyl-protein thioesterase 1 (APT1), also known as lysophospholipase A-I (LYPLA-I; accession code O75608) as a novel PLA(1). Biotin 181-187 coagulation factor II, thrombin Homo sapiens 72-80 34075739-7 2021 The biotin ends of the aptamers were attached to the microparticles through streptavidin-biotin linkage, whereas the free ends of the aptamers were used to capture their target proteins of thrombin (th) and immunoglobulin E (IgE). Biotin 4-10 coagulation factor II, thrombin Homo sapiens 189-197 12724354-6 2003 This was confirmed by observing that thrombin pretreatment of HUVECs increased biotin-modified plasminogen binding. Biotin 79-85 coagulation factor II, thrombin Homo sapiens 37-45 9134755-1 1997 Human alpha-thrombin and Factor Xa were acylated at their active site serine hydroxyls with biotin-substituted cinnamate derivatives 1b-1c. Biotin 92-98 coagulation factor II, thrombin Homo sapiens 12-20 9134755-6 1997 Furthermore, the complex formed between the thrombin acyl enzyme (2c) and avidin was capable of binding to immobilized biotin. Biotin 119-125 coagulation factor II, thrombin Homo sapiens 44-52 11554721-0 2001 Biotin derivatives of D-Phe-Pro-Arg-CH2Cl for active-site-specific labeling of thrombin and other serine proteinases. Biotin 0-6 coagulation factor II, thrombin Homo sapiens 79-87 11554721-2 2001 To make the reagents more accessible, a simple, economical method was developed for preparation of three biotin derivatives of the thrombin-specific inhibitor D-Phe-Pro-Arg-CH2Cl containing increasing lengths of the spacer connecting biotin. Biotin 105-111 coagulation factor II, thrombin Homo sapiens 131-139 11554721-2 2001 To make the reagents more accessible, a simple, economical method was developed for preparation of three biotin derivatives of the thrombin-specific inhibitor D-Phe-Pro-Arg-CH2Cl containing increasing lengths of the spacer connecting biotin. Biotin 234-240 coagulation factor II, thrombin Homo sapiens 131-139 11554721-4 2001 The biotin-labeled inhibitors bound avidin with stoichiometries of 0.88-1.02 mol biotin compound/mol avidin subunits and irreversibly inactivated human thrombin with stoichiometries of 0.89-1.10 mol inhibitor/mol thrombin. Biotin 4-10 coagulation factor II, thrombin Homo sapiens 152-160 11554721-4 2001 The biotin-labeled inhibitors bound avidin with stoichiometries of 0.88-1.02 mol biotin compound/mol avidin subunits and irreversibly inactivated human thrombin with stoichiometries of 0.89-1.10 mol inhibitor/mol thrombin. Biotin 4-10 coagulation factor II, thrombin Homo sapiens 213-221 7656454-6 1995 The reagents were inhibited biotinylated thrombin, anti-biotin monoclonal antibody, and PIOP. Biotin 28-34 coagulation factor II, thrombin Homo sapiens 41-49 31594565-4 2020 Thrombin bound to one biotinylated aptamer, and then the thrombin-labeled aptamer was attached on the sandwich complex through streptavidin-biotin interaction by using streptavidin as a linker. Biotin 22-28 coagulation factor II, thrombin Homo sapiens 0-8