PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8819333-6 1996 Expression of BIO2 was higher in the light relative to dark and was induced 5-fold during biotin-limited conditions. Biotin 90-96 Radical SAM superfamily protein Arabidopsis thaliana 14-18 33180716-5 2020 Additionally, a carbonate treatment increased the endogenous biotin content and expression of AtBIO2, which encodes a biotin synthase. Biotin 61-67 Radical SAM superfamily protein Arabidopsis thaliana 118-133 8680961-0 1996 Biotin synthesis in higher plants: isolation of a cDNA encoding Arabidopsis thaliana bioB-gene product equivalent by functional complementation of a biotin auxotroph mutant bioB105 of Escherichia coli K12. Biotin 0-6 Radical SAM superfamily protein Arabidopsis thaliana 85-89 8680961-0 1996 Biotin synthesis in higher plants: isolation of a cDNA encoding Arabidopsis thaliana bioB-gene product equivalent by functional complementation of a biotin auxotroph mutant bioB105 of Escherichia coli K12. Biotin 149-155 Radical SAM superfamily protein Arabidopsis thaliana 85-89 8680961-2 1996 We isolated a complete cDNA (1.3 kb) encoding A. thaliana bioB-gene product by functional complementation of the bioB105 biotin auxotroph mutant of Escherichia coli K12 using an A. thaliana cDNA library. Biotin 121-127 Radical SAM superfamily protein Arabidopsis thaliana 58-62 23031218-6 2013 In addition, we show that SUC5 is essential for the delivery of biotin into the embryo of biotin biosynthesis-defective mutants (bio1 and bio2). Biotin 64-70 Radical SAM superfamily protein Arabidopsis thaliana 138-142 23031218-6 2013 In addition, we show that SUC5 is essential for the delivery of biotin into the embryo of biotin biosynthesis-defective mutants (bio1 and bio2). Biotin 90-96 Radical SAM superfamily protein Arabidopsis thaliana 138-142 9428635-0 1997 Biotin synthesis in higher plants: purification and characterization of bioB gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells. Biotin 0-6 Radical SAM superfamily protein Arabidopsis thaliana 72-76 9428635-1 1997 Biotin synthase catalyses the final step in the biotin biosynthetic pathway and is encoded by the bioB gene in Escherichia coli. Biotin 48-54 Radical SAM superfamily protein Arabidopsis thaliana 98-102 16897469-3 2006 The last step in biotin biosynthesis occurs within the mitochondria and is catalyzed by the biotin synthase complex containing the BIO2 gene product. Biotin 17-23 Radical SAM superfamily protein Arabidopsis thaliana 131-135 16897469-5 2006 We describe the characterization of a new T-DNA allele of bio2, with the sole phenotype of an absence of biotin production, in contrast to the previously characterized EMS bio2 allele (Patton et al. Biotin 105-111 Radical SAM superfamily protein Arabidopsis thaliana 58-62 16897469-10 2006 Bio2 requires mitochondrial targeting for activity, enabling it to fulfill its role in biotin synthesis. Biotin 87-93 Radical SAM superfamily protein Arabidopsis thaliana 0-4 11706201-2 2001 Biotin synthase, encoded by the bio2 gene in Arabidopsis, catalyzes the final step in the biotin biosynthetic pathway. Biotin 90-96 Radical SAM superfamily protein Arabidopsis thaliana 0-15 11706201-2 2001 Biotin synthase, encoded by the bio2 gene in Arabidopsis, catalyzes the final step in the biotin biosynthetic pathway. Biotin 90-96 Radical SAM superfamily protein Arabidopsis thaliana 32-36 11706201-8 2001 In vitro inhibition of biotin synthase activity by acidomycin, a structural analog of biotin, showed that biotin synthase reaction was the specific target of this inhibitor of biotin synthesis. Biotin 23-29 Radical SAM superfamily protein Arabidopsis thaliana 106-121 11706201-8 2001 In vitro inhibition of biotin synthase activity by acidomycin, a structural analog of biotin, showed that biotin synthase reaction was the specific target of this inhibitor of biotin synthesis. Biotin 86-92 Radical SAM superfamily protein Arabidopsis thaliana 23-38 11706201-8 2001 In vitro inhibition of biotin synthase activity by acidomycin, a structural analog of biotin, showed that biotin synthase reaction was the specific target of this inhibitor of biotin synthesis. Biotin 86-92 Radical SAM superfamily protein Arabidopsis thaliana 106-121 11706201-9 2001 It is important that combination experiments using purified Bio2 protein and extracts from pea (Pisum sativum) leaf or potato (Solanum tuberosum) organelles showed that only mitochondrial fractions could elicit biotin formation in the plant-reconstituted system. Biotin 211-217 Radical SAM superfamily protein Arabidopsis thaliana 60-64 11706201-10 2001 Our data demonstrated that one or more unidentified factors from mitochondrial matrix (pea and potato) and from mitochondrial membranes (pea), in addition to the Bio2 protein, are obligatory for the conversion of dethiobiotin to biotin, highlighting the importance of mitochondria in plant biotin synthesis. Biotin 219-225 Radical SAM superfamily protein Arabidopsis thaliana 162-166 11706201-10 2001 Our data demonstrated that one or more unidentified factors from mitochondrial matrix (pea and potato) and from mitochondrial membranes (pea), in addition to the Bio2 protein, are obligatory for the conversion of dethiobiotin to biotin, highlighting the importance of mitochondria in plant biotin synthesis. Biotin 229-235 Radical SAM superfamily protein Arabidopsis thaliana 162-166 9501126-3 1998 Arrested embryos from this bio2 mutant are defective in the final step of biotin synthesis, the conversion of dethiobiotin to biotin. This enzymatic reaction, catalyzed by the bioB product (biotin synthase) in Escherichia coli, has been studied extensively in plants and bacteria because it involves the unusual addition of sulfur to form a thiophene ring. Biotin 75-81 Radical SAM superfamily protein Arabidopsis thaliana 28-32 9501126-3 1998 Arrested embryos from this bio2 mutant are defective in the final step of biotin synthesis, the conversion of dethiobiotin to biotin. This enzymatic reaction, catalyzed by the bioB product (biotin synthase) in Escherichia coli, has been studied extensively in plants and bacteria because it involves the unusual addition of sulfur to form a thiophene ring. Biotin 75-81 Radical SAM superfamily protein Arabidopsis thaliana 191-206 9501126-3 1998 Arrested embryos from this bio2 mutant are defective in the final step of biotin synthesis, the conversion of dethiobiotin to biotin. This enzymatic reaction, catalyzed by the bioB product (biotin synthase) in Escherichia coli, has been studied extensively in plants and bacteria because it involves the unusual addition of sulfur to form a thiophene ring. Biotin 127-133 Radical SAM superfamily protein Arabidopsis thaliana 28-32 9501126-3 1998 Arrested embryos from this bio2 mutant are defective in the final step of biotin synthesis, the conversion of dethiobiotin to biotin. This enzymatic reaction, catalyzed by the bioB product (biotin synthase) in Escherichia coli, has been studied extensively in plants and bacteria because it involves the unusual addition of sulfur to form a thiophene ring. Biotin 127-133 Radical SAM superfamily protein Arabidopsis thaliana 191-206 9501126-4 1998 Three lines of evidence indicate that bio2 is defective in biotin synthase production: mutant embryos are rescued by biotin but not dethiobiotin, the mutant allele maps to the same chromosomal location as the cloned biotin synthase gene, and gel-blot hybridizations and polymerase chain reaction amplifications revealed that homozygous mutant plants contain a deletion spanning the entire BIO2-coding region. Biotin 60-66 Radical SAM superfamily protein Arabidopsis thaliana 39-43 9428635-2 1997 To investigate the conversion of dethiobiotin to biotin in the plant kingdom, the cDNA encoding the bioB gene product equivalent from Arabidopsis thaliana was used to construct an E. coli overexpression strain. Biotin 39-45 Radical SAM superfamily protein Arabidopsis thaliana 100-104