PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
23924735-5	2013	To characterize this adaptor function, initially we investigated the binding of ALG-2 to ESCRT-I complexes containing each one of the four different VPS37 isoforms by two approaches: first, Far-Western blot analysis with biotin-labeled ALG-2 probe, and second, a pulldown assay to determine the binding of the four recombinant ESCRT-I complexes to Strep-tagged ALG-2 after co-expression in HEK293T cells.	Biotin	221-227	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	80-85	
20834162-4	2010	Next, overlay experiments with biotin-labeled ALG-2 were done to dissect the ALG-2 binding site (ABS).	Biotin	31-37	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	46-51	
14999017-2	2004	The yeast two-hybrid system and a biotin-tagged ALG-2 overlay assay were carried out to characterize the interaction between ALG-2 and Alix.	Biotin	34-40	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	125-130	
12445460-6	2002	Using recombinant ALG-2 proteins and the glutathione S-transferase (GST) fusion proteins of Anx7N and Anx11N, the direct Ca(2+)-dependent interaction was analyzed by a biotin-tagged ALG-2 overlay assay and by a real-time interaction analysis with a surface plasmon resonance (SPR) biosensor.	Biotin	168-174	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	18-23	
18256029-3	2008	In the present study, we found that ALG-2 interacts with PLSCR3 expressed in HEK293 cells in a Ca(2+)-dependent manner by co-immunoprecipitation, pulldown with glutathione S-transferase (GST) fused ALG-2 and an overlay assay using biotin-labeled ALG-2.	Biotin	231-237	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	36-41	
17196169-3	2007	In vitro GST pull down analysis demonstrated that ALG-2 and its alternatively spliced isoform interact with the COPII component Sec31A in a Ca2+-dependent manner, and a biotin-labeled ALG-2 overlay assay revealed direct binding of ALG-2 to Sec31A.	Biotin	169-175	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	50-55	
17196169-3	2007	In vitro GST pull down analysis demonstrated that ALG-2 and its alternatively spliced isoform interact with the COPII component Sec31A in a Ca2+-dependent manner, and a biotin-labeled ALG-2 overlay assay revealed direct binding of ALG-2 to Sec31A.	Biotin	169-175	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	184-189	
17196169-3	2007	In vitro GST pull down analysis demonstrated that ALG-2 and its alternatively spliced isoform interact with the COPII component Sec31A in a Ca2+-dependent manner, and a biotin-labeled ALG-2 overlay assay revealed direct binding of ALG-2 to Sec31A.	Biotin	169-175	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	184-189	
16004603-7	2005	Direct binding of ALG-2 to the TSG101 PRR was demonstrated by an overlay assay using biotin-labelled ALG-2 as a probe.	Biotin	85-91	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	18-23	
16004603-7	2005	Direct binding of ALG-2 to the TSG101 PRR was demonstrated by an overlay assay using biotin-labelled ALG-2 as a probe.	Biotin	85-91	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	101-106	
11883939-5	2002	Using recombinant biotin-tagged ALG-2 and the glutathione S-transferase (GST) fusion protein of AnxN, the direct interaction was analyzed by an ALG-2 overlay assay and by real-time interaction analysis with a surface plasmon resonance (SPR) biosensor.	Biotin	18-24	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	32-37	
11883939-5	2002	Using recombinant biotin-tagged ALG-2 and the glutathione S-transferase (GST) fusion protein of AnxN, the direct interaction was analyzed by an ALG-2 overlay assay and by real-time interaction analysis with a surface plasmon resonance (SPR) biosensor.	Biotin	18-24	ALG2 alpha-1,3/1,6-mannosyltransferase	Homo sapiens	144-149