Title : Intracellular UDP-N-acetylhexosamine pool affects N-glycan complexity: a mechanism of ammonium action on protein glycosylation.

Pub. Date : 1998 May-Jun

PMID : 9622521






1 Functional Relationships(s)
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1 To investigate this novel hypothesis, we induced an artificial increase in UDPGNAc pool by treating recombinant BHK cells expressing an IL-2 variant that features an artificial site for N-glycosylation, with glucosamine (1:2 molar ratio to glucose) and uridine (2 mmol L-1) in the absence of ammonium ions or glutamine. Uridine Diphosphate N-Acetylglucosamine interleukin-2 Mesocricetus auratus