Title : Side chain hydroxylations in biosynthesis of cholic acid. 25- and 26-Hydroxylation of 5beta-cholestane-3alpha, 7alpha, 12alpha-triol by reconstituted systems from rat liver microsomes.

Pub. Date : 1976 Jun 10

PMID : 931995






2 Functional Relationships(s)
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Protein Name
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1 Cytochrome P -450 was prepared either by sodium cholate treatment and ammonium sulfate fractionation or by subtilisin and sodium deoxycholate treatment followed by DEAE-cellulose chromatography. Sodium Cholate cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus
2 The use of increasing amounts of sodium cholate in the solubilization of cytochrome P -450 resulted in a gradual decrease of 25-hydroxylase activity and a gradual increase of 26-hydroxylase activity. Sodium Cholate cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus