Title : Preparation of immobilized baker's-yeast glucose 6-phosphate dehydrogenase attached to modified sepharose and sephadex and a comparison of the properties of these preparations with those of the soluble enzyme.

Pub. Date : 1976 Aug 1

PMID : 9068






2 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate-NADP+ oxidoreductase, EC 1.1.1.49) from baker"s yeast (Saccharomyces cerevisiae) was immobilized on CNBr-activated Sepharose 4B with retention of about 3% of enzyme activity. Sepharose glucose-6-phosphate dehydrogenase Saccharomyces cerevisiae S288C
2 Glucose 6-phosphate dehydrogenase immobilized on CNBr-activated Sephadex G-25 was unstable, as was enzyme attached to CNBr-activated Sepharose 4B to which glycine, asparitic acid, valine or ethylenediamine was added at the same time as the enzyme. Sepharose glucose-6-phosphate dehydrogenase Saccharomyces cerevisiae S288C