Title : Regulation of phosphorylation of deoxycytidine and 2',2'-difluorodeoxycytidine (gemcitabine); effects of cytidine 5'-triphosphate and uridine 5'-triphosphate in relation to chemosensitivity for 2',2'-difluorodeoxycytidine.

Pub. Date : 1996 Apr 12

PMID : 8651941






3 Functional Relationships(s)
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1 dCK and dCDA activities were measured with both deoxycytidine and dFdC as substrates in 5 solid tumour cell lines, but no correlation with cellular sensitivity to dFdC was found with either substrate. gemcitabine Calcium/calmodulin-dependent protein kinase II Drosophila melanogaster
2 In conclusion, the efficiency of dCK (Vmax/Km ratio) seems to correlate with accumulation of dFdCTP, the active metabolite of dFdC, and with cellular sensitivity. gemcitabine Calcium/calmodulin-dependent protein kinase II Drosophila melanogaster
3 Both activation and inhibition have been observed; the physiologically low CTP pools and the relatively minor effect on dCK in A2780 cells seem to favour dFdC phosphorylation in these cells, which are the most sensitive. gemcitabine Calcium/calmodulin-dependent protein kinase II Drosophila melanogaster