Pub. Date : 1994 Aug
PMID : 7518560
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Results of co-immunoprecipitation experiments suggest that phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. | phosphotyrosines y-1068 | growth factor receptor bound protein 2 | Homo sapiens |
| 2 | Results of co-immunoprecipitation experiments suggest that phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. | Y-1173 | growth factor receptor bound protein 2 | Homo sapiens |
| 3 | Competition experiments with synthetic phosphopeptides corresponding to known autophosphorylation sites on the EGFR demonstrated that phosphopeptides containing Y-1068, and to a lesser extent Y-1086, were able to inhibit the binding of Grb2 to the EGFR, while a Y-1173 peptide did not. | y-1068 | growth factor receptor bound protein 2 | Homo sapiens |
| 4 | These findings were confirmed by using a dephosphorylation protection assay and by measuring the dissociation constants of Grb2"s SH2 domain to tyrosine-phosphorylated peptides, using real-time biospecific interaction analysis (BIAcore). | Tyrosine | growth factor receptor bound protein 2 | Homo sapiens |
| 5 | These analyses reveal a hierarchy of interactions between Grb2 and Shc with the EGFR and indicate that Grb2 can bind the tyrosine-phosphorylated EGFR directly, as well as indirectly via Shc. | Tyrosine | growth factor receptor bound protein 2 | Homo sapiens |