Title : Improved method for isolating synaptosomes from 11 regions of one rat brain: electron microscopic and biochemical characterization and use in the study of drug effects on nerve terminal gamma-aminobutyric acid in vivo.

Pub. Date : 1985 Sep

PMID : 3928810






1 Functional Relationships(s)
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1 The postmortem increase in GABA level during removal and dissection of brain tissue and homogenization and fractionation procedures could be minimized by rapid processing of the tissue at low temperatures and inclusion of the GAD inhibitor 3-mercaptopropionic acid (3-MP; 1 mM) in the homogenizing medium. 3-Mercaptopropionic Acid glutamate-ammonia ligase Rattus norvegicus