Title : Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells.

Pub. Date : 1987 Oct

PMID : 3479680






3 Functional Relationships(s)
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1 Stepwise selection of transfected CHO cells in increasing concentrations of methotrexate yielded cell lines that expressed amplified TGF-beta nucleic acid sequences. Methotrexate transforming growth factor, beta 1 Mus musculus
2 Antibodies directed toward sequences present in the mature growth factor readily identified a proteolytically processed recombinant TGF-beta which, on sodium dodecyl sulfate-polyacrylamide gels, comigrated with highly purified natural TGF-beta. Sodium Dodecyl Sulfate transforming growth factor, beta 1 Mus musculus
3 Antibodies directed toward sequences present in the mature growth factor readily identified a proteolytically processed recombinant TGF-beta which, on sodium dodecyl sulfate-polyacrylamide gels, comigrated with highly purified natural TGF-beta. polyacrylamide transforming growth factor, beta 1 Mus musculus