Pub. Date : 1989 Aug 1
PMID : 2508623
13 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In plasma membranes from AKR-2B cells, TGF beta 1 increased binding of the radiolabelled, non-hydrolysable GTP analogue, guanosine 5"-[gamma-[35S]thio]triphosphate (GTP[35S]), in a dose-dependent manner. | Guanosine Triphosphate | transforming growth factor, beta 1 | Mus musculus |
| 2 | In plasma membranes from AKR-2B cells, TGF beta 1 increased binding of the radiolabelled, non-hydrolysable GTP analogue, guanosine 5"-[gamma-[35S]thio]triphosphate (GTP[35S]), in a dose-dependent manner. | guanosine 5"-[gamma-[35s]thio]triphosphate | transforming growth factor, beta 1 | Mus musculus |
| 3 | In plasma membranes from AKR-2B cells, TGF beta 1 increased binding of the radiolabelled, non-hydrolysable GTP analogue, guanosine 5"-[gamma-[35S]thio]triphosphate (GTP[35S]), in a dose-dependent manner. | Guanosine Triphosphate | transforming growth factor, beta 1 | Mus musculus |
| 4 | In plasma membranes from AKR-2B cells, TGF beta 1 increased binding of the radiolabelled, non-hydrolysable GTP analogue, guanosine 5"-[gamma-[35S]thio]triphosphate (GTP[35S]), in a dose-dependent manner. | Sulfur-35 | transforming growth factor, beta 1 | Mus musculus |
| 5 | Instead, TGF beta 1 increased the number of available binding sites for GTP[35S] from 16.2 +/- 1.2 to 21.6 +/- 2.1 pmol/mg of protein. | Guanosine Triphosphate | transforming growth factor, beta 1 | Mus musculus |
| 6 | Instead, TGF beta 1 increased the number of available binding sites for GTP[35S] from 16.2 +/- 1.2 to 21.6 +/- 2.1 pmol/mg of protein. | Sulfur-35 | transforming growth factor, beta 1 | Mus musculus |
| 7 | Only guanine nucleotides were able to compete for binding, and of the growth factors tested (epidermal growth factor, platelet-derived growth factor, insulin, TGF beta 1 and TGF beta 2) only TGF beta 1 affected GTP[35S] binding. | Guanosine Triphosphate | transforming growth factor, beta 1 | Mus musculus |
| 8 | Only guanine nucleotides were able to compete for binding, and of the growth factors tested (epidermal growth factor, platelet-derived growth factor, insulin, TGF beta 1 and TGF beta 2) only TGF beta 1 affected GTP[35S] binding. | Sulfur-35 | transforming growth factor, beta 1 | Mus musculus |
| 9 | TGF beta 1 increased GTPase activity, as determined by the release of 32PO4(3-) from GTP gamma[32P], from 116 +/- 5.5 to 175 +/- 4.3 pmol/mg of protein following a 15 min incubation. | 32po4 | transforming growth factor, beta 1 | Mus musculus |
| 10 | TGF beta 1 increased GTPase activity, as determined by the release of 32PO4(3-) from GTP gamma[32P], from 116 +/- 5.5 to 175 +/- 4.3 pmol/mg of protein following a 15 min incubation. | Guanosine Triphosphate | transforming growth factor, beta 1 | Mus musculus |
| 11 | TGF beta 1 increased GTPase activity, as determined by the release of 32PO4(3-) from GTP gamma[32P], from 116 +/- 5.5 to 175 +/- 4.3 pmol/mg of protein following a 15 min incubation. | Phosphorus-32 | transforming growth factor, beta 1 | Mus musculus |
| 12 | Pretreatment of the membranes with pertussis toxin inhibited both TGF beta 1-stimulated binding of GTP[35S] as well as TGF beta 1-stimulated GTPase activity. | Guanosine Triphosphate | transforming growth factor, beta 1 | Mus musculus |
| 13 | Pretreatment of the membranes with pertussis toxin inhibited both TGF beta 1-stimulated binding of GTP[35S] as well as TGF beta 1-stimulated GTPase activity. | Sulfur-35 | transforming growth factor, beta 1 | Mus musculus |