Title : Divergent effects of cycloheximide on the induction of class II and class III cytochrome P450 mRNAs in cultures of adult rat hepatocytes.

Pub. Date : 1990 Sep

PMID : 2393297






2 Functional Relationships(s)
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1 Analysis of Northern blots hybridized with oligonucleotides specific for P450PCN1(IIIA1) and P450PCN2(IIIA2), respectively, revealed that untreated male rat liver and cultures of hepatocytes prepared from these animals expressed readily detectable amounts of P450PCN1(IIIA1) mRNA. Oligonucleotides cytochrome P450, family 3, subfamily a, polypeptide 2 Rattus norvegicus
2 We conclude that the pathways for the induction of P450b/e and P450p by phenobarbital, and the pathways for the gender-specific basal expression of P450PCN1(IIIA1) and P450PCN2(IIIA2) are not the same and can be distinguished by their differential response to inhibition of ongoing protein synthesis. Phenobarbital cytochrome P450, family 3, subfamily a, polypeptide 2 Rattus norvegicus