Title : Separation in a single step by affinity chromatography of cholinesterases differing in subunit number.

Pub. Date : 1991 Aug

PMID : 1821797






2 Functional Relationships(s)
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1 We describe an affinity chromatography method in which dimethylaminoethylbenzoic acid-Sepharose 4B is used, making it possible to separate in one step the molecular forms of globular acetylcholinesterase (AChE, EC 3.1.1.7) or butyrylcholinesterase (ChE, EC 3.1.1.8). dimethylaminoethylbenzoic acid-sepharose 4b acetylcholinesterase Rattus norvegicus
2 With rat brain AChE, two well-separated peaks were eluted in the presence of 1% Triton X-100; the first peak corresponded to 4 S forms and the second to 11 S forms. Octoxynol acetylcholinesterase Rattus norvegicus