Title : Reversible oxidative activation and inactivation of protein kinase C by the mitogen/tumor promoter periodate.

Pub. Date : 1991 Mar

PMID : 1654774






9 Functional Relationships(s)
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1 The oxidant mitogen/tumor promoter, periodate, was used to selectively modify either the regulatory domain or the catalytic domain of protein kinase C (PKC) to induce oxidative activation or inactivation of PKC, respectively. metaperiodate proline rich transmembrane protein 2 Homo sapiens
2 The oxidant mitogen/tumor promoter, periodate, was used to selectively modify either the regulatory domain or the catalytic domain of protein kinase C (PKC) to induce oxidative activation or inactivation of PKC, respectively. metaperiodate proline rich transmembrane protein 2 Homo sapiens
3 The oxidant mitogen/tumor promoter, periodate, was used to selectively modify either the regulatory domain or the catalytic domain of protein kinase C (PKC) to induce oxidative activation or inactivation of PKC, respectively. metaperiodate proline rich transmembrane protein 2 Homo sapiens
4 Periodate, at micromolar concentrations, modified the regulatory domain of PKC as determined by the loss of ability to stimulate kinase activity by Ca2+/phospholipid, and also by the loss of phorbol ester binding. metaperiodate proline rich transmembrane protein 2 Homo sapiens
5 However, at higher concentrations (greater than 100 microM) periodate also modified the catalytic domain, resulting in complete inactivation of PKC. metaperiodate proline rich transmembrane protein 2 Homo sapiens
6 In this aspect, the modification induced by periodate was different from that of the previously reported irreversible modification of PKC induced by H2O2. metaperiodate proline rich transmembrane protein 2 Homo sapiens
7 However, the inactivation of PKC induced by periodate at concentrations greater than 1 mM was not reversed by dithiothreitol. metaperiodate proline rich transmembrane protein 2 Homo sapiens
8 In the presence of phosphatidylserine, the catalytic site was selectively modified by periodate, resulting in formation of a form of PKC that exhibited phorbol ester binding but not kinase activity. metaperiodate proline rich transmembrane protein 2 Homo sapiens
9 Both reversible and irreversible oxidative activation and inactivation of PKC also were observed in intact cells treated with periodate. metaperiodate proline rich transmembrane protein 2 Homo sapiens