Pub. Date : 2006 May 15
PMID : 16460309
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Activation of the superoxide-producing phagocyte NADPH oxidase requires co-operation between the tandem SH3 domains of p47phox in recognition of a polyproline type II helix and an adjacent alpha-helix of p22phox. | Superoxides | cytochrome b-245 alpha chain | Homo sapiens |
| 2 | Activation of the superoxide-producing phagocyte NADPH oxidase, crucial for host defence, requires an SH3 (Src homology 3)-domain-mediated interaction of the regulatory protein p47phox with p22phox, a subunit of the oxidase catalytic core flavocytochrome b558. | Superoxides | cytochrome b-245 alpha chain | Homo sapiens |
| 3 | Although previous analysis of a crystal structure has demonstrated that the tandem SH3 domains of p47phox sandwich a short PRR (proline-rich region) of p22phox (amino acids 151-160), containing a polyproline II helix, it has remained unknown whether this model is indeed functional in activation of the oxidase. | Proline | cytochrome b-245 alpha chain | Homo sapiens |
| 4 | Although previous analysis of a crystal structure has demonstrated that the tandem SH3 domains of p47phox sandwich a short PRR (proline-rich region) of p22phox (amino acids 151-160), containing a polyproline II helix, it has remained unknown whether this model is indeed functional in activation of the oxidase. | polyproline | cytochrome b-245 alpha chain | Homo sapiens |
| 5 | Deletion of the linker between the p47phox SH3 domains results not only in a defective binding to p22phox but also in a loss of the activity to support superoxide production. | Superoxides | cytochrome b-245 alpha chain | Homo sapiens |
| 6 | The present analysis using alanine-scanning mutagenesis identifies Pro152, Pro156 and Arg158 in the p22phox PRR as residues indispensable for the interaction with p47phox. | Alanine | cytochrome b-245 alpha chain | Homo sapiens |
| 7 | Amino acid substitution for any of the three residues in the p22phox PRR abrogates the superoxide-producing activity of the oxidase reconstituted in intact cells. | Superoxides | cytochrome b-245 alpha chain | Homo sapiens |