Pub. Date : 2000 Nov
PMID : 11040087
11 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In order to define the ability of bovine lens aldose reductase (ALR2) to generate polyols from aldoses, the quantitative determination of glycerol in the presence of glyceraldehyde was performed by gas chromatography after derivatization with trifluoroacetic anhydride. | Glycerol | lens aldose reductase pseudogene | Bos taurus |
| 2 | In order to define the ability of bovine lens aldose reductase (ALR2) to generate polyols from aldoses, the quantitative determination of glycerol in the presence of glyceraldehyde was performed by gas chromatography after derivatization with trifluoroacetic anhydride. | Glyceraldehyde | lens aldose reductase pseudogene | Bos taurus |
| 3 | In order to define the ability of bovine lens aldose reductase (ALR2) to generate polyols from aldoses, the quantitative determination of glycerol in the presence of glyceraldehyde was performed by gas chromatography after derivatization with trifluoroacetic anhydride. | trifluoroacetic anhydride | lens aldose reductase pseudogene | Bos taurus |
| 4 | The generation of one equivalent of glycerol in the presence of ALR2, is paralleled by the oxidation of one equivalent of NADPH. | Glycerol | lens aldose reductase pseudogene | Bos taurus |
| 5 | The generation of one equivalent of glycerol in the presence of ALR2, is paralleled by the oxidation of one equivalent of NADPH. | NADP | lens aldose reductase pseudogene | Bos taurus |
| 6 | A similar result was obtained when S-glutathionyl-modified ALR2 was used, instead of the native enzyme, as a catalyst of glyceraldehyde reduction. | s-glutathionyl | lens aldose reductase pseudogene | Bos taurus |
| 7 | Sorbinil, a classical ALR2 inhibitor, present in the enzyme assay mixture, inhibits to the same extent both NADPH oxidation and glycerol formation. | sorbinil | lens aldose reductase pseudogene | Bos taurus |
| 8 | The demonstration of the stoichiometric ratio of 1:1 occurring in the presence of bovine lens ALR2 between the synthesis of glycerol from D, L -glyceraldehyde and the oxidation of NADPH, rules out doubts concerning the ability of the enzyme to catalyse the reduction of aldoses to the corresponding polyalcohols. | Glycerol | lens aldose reductase pseudogene | Bos taurus |
| 9 | The demonstration of the stoichiometric ratio of 1:1 occurring in the presence of bovine lens ALR2 between the synthesis of glycerol from D, L -glyceraldehyde and the oxidation of NADPH, rules out doubts concerning the ability of the enzyme to catalyse the reduction of aldoses to the corresponding polyalcohols. | d, l -glyceraldehyde | lens aldose reductase pseudogene | Bos taurus |
| 10 | The demonstration of the stoichiometric ratio of 1:1 occurring in the presence of bovine lens ALR2 between the synthesis of glycerol from D, L -glyceraldehyde and the oxidation of NADPH, rules out doubts concerning the ability of the enzyme to catalyse the reduction of aldoses to the corresponding polyalcohols. | polyalcohols | lens aldose reductase pseudogene | Bos taurus |
| 11 | This would indicate that the spectrophotometric monitoring of NADPH oxidation at 340 nm, in the presence of ALR2, is a reliable method to assay the enzyme activity. | NADP | lens aldose reductase pseudogene | Bos taurus |