Pub. Date : 1998 Sep
PMID : 9801832
2 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | As to the catalytic mechanism of CPA, it is generally believed that while in the cases of ester substrates the carboxylate of Glu-270 functions as the nucleophile which attacks the scissile carbonyl carbon (anhydride pathway), in the case of peptide substrates the zinc-bound water molecule attacks the scissile peptide bond (general base pathway). | carboxylate | carboxypeptidase A1 | Homo sapiens |
| 2 | We have also demonstrated that N-(hydroxyacetyl)-L-phenylalanine (L-2) inactivates the enzyme with the kinact/KI value of 0.071 M-1 s-1, suggesting that the carboxylate becomes to attack the peptide carbonyl carbon to generate the same anhydride intermediate as that formed in the inactivation of CPA by L-1. | carboxylate | carboxypeptidase A1 | Homo sapiens |