Title : Optimization and comparison of the MTT assay and the 3H-TdR assay for the detection of IL-2 in helper T cell precursor assays.

Pub. Date : 1998 Aug 1

PMID : 9776586






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1 Optimization and comparison of the MTT assay and the 3H-TdR assay for the detection of IL-2 in helper T cell precursor assays. Tritium interleukin 2 Homo sapiens
2 Due to the different measurement procedures, the volumes of the IL-2 dilutions were 75 microl in assays with MTT and 150 microl in assays with 3H-TdR. Tritium interleukin 2 Homo sapiens
3 Because of the possibility of IL-2 detection in the whole culture volume (150 microl), we found that the 3H-TdR assay was superior to the MTT assay with a 10-fold better sensitivity in different human sera. Tritium interleukin 2 Homo sapiens