Title : Advantages of immunostaining over DNA analysis using PCR amplification to detect p53 abnormality in long-term formalin-fixed tissues of human colorectal carcinomas.

Pub. Date : 1998 Oct

PMID : 9773930






5 Functional Relationships(s)
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1 Advantages of immunostaining over DNA analysis using PCR amplification to detect p53 abnormality in long-term formalin-fixed tissues of human colorectal carcinomas. Formaldehyde tumor protein p53 Homo sapiens
2 To study the appropriate period for formalin fixation in order to detect p53 abnormalities in formalin-fixed tissue, we used seven surgically resected human colorectal cancer specimens. Formaldehyde tumor protein p53 Homo sapiens
3 To study the appropriate period for formalin fixation in order to detect p53 abnormalities in formalin-fixed tissue, we used seven surgically resected human colorectal cancer specimens. Formaldehyde tumor protein p53 Homo sapiens
4 PCR amplification for p53 at exon 8 and K-ras at codon 12 was successful until 1 day and 2 weeks, respectively, of formalin-fixation for the specimens of all seven cases. Formaldehyde tumor protein p53 Homo sapiens
5 These results suggest that to detect p53 abnormality in specimens that have been formalin-fixed for long periods, immunohistochemical staining may have advantages over DNA analysis with PCR amplification. Formaldehyde tumor protein p53 Homo sapiens