Title : Probing the effects of calcium on gelsolin.

Pub. Date : 1997 Dec 16

PMID : 9398317






9 Functional Relationships(s)
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Protein Name
Organism
1 Probing the effects of calcium on gelsolin. Calcium gelsolin Homo sapiens
2 Gelsolin is a calcium-regulated actin severing and capping protein that binds two calcium ions and has three sites for actin; two recognize monomeric actin and one attaches to the sides of filaments. Calcium gelsolin Homo sapiens
3 Gelsolin is a calcium-regulated actin severing and capping protein that binds two calcium ions and has three sites for actin; two recognize monomeric actin and one attaches to the sides of filaments. Calcium gelsolin Homo sapiens
4 Here, we have analyzed the effects of calcium ions on (i) limited proteolysis of bacterially expressed human gelsolin by plasmin and (ii) dynamic light scattering and circular dichroism of gelsolin and various of its subdomains. Calcium gelsolin Homo sapiens
5 Following cleavage of gelsolin in the absence of calcium between Lys150 and His151 (the junction between G1 and G2), the molecule does not fall apart, nor does it bind actin without added calcium. Calcium gelsolin Homo sapiens
6 The noncovalently linked form of gelsolin shows three actin binding sites in calcium and requires 3 microM calcium for 50% activation of actin binding. Calcium gelsolin Homo sapiens
7 The noncovalently linked form of gelsolin shows three actin binding sites in calcium and requires 3 microM calcium for 50% activation of actin binding. Calcium gelsolin Homo sapiens
8 Measurements of light scattering and circular dichroism revealed structural changes in response to calcium for intact gelsolin and a number of its actin-binding subdomains. Calcium gelsolin Homo sapiens
9 These results are discussed in relation to the calcium control of gelsolin function and its three-dimensional structure (Burtnick et al. Calcium gelsolin Homo sapiens