Title : Amidated carboxyl groups in elastin.

Pub. Date : 1976 May 20

PMID : 938666






1 Functional Relationships(s)
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1 Acid-labile amide-bound ammonia of elastin was quantitated after hydrolysis of the insoluble protein with 2 M HC1 by incubating aliquots of microdistilled hydrolysates with glutamate dehydrogenase, excess alpha-ketoglutarate, and reduced nicotinamide adenine dinucleotide and measuring the resultant decrease in A340 due to oxidation of the dinucleotide cofactor. Ketoglutaric Acids elastin Homo sapiens