Title : Identification of an adenine-nucleotide-binding site on interferon alpha2.

Pub. Date : 1997 Aug 1

PMID : 9288895






6 Functional Relationships(s)
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1 Using 32P-labeled 2-azidoadenosine 5"-triphosphate (2N3ATP) and 8-azidoadenosine 5"-triphosphate (8N3ATP), we have identified a site on human interferon alpha2 (IFN-alpha2) that binds adenine nucleotides. 8-azidoadenosine 5'-triphosphate interferon alpha 2 Homo sapiens
2 Using 32P-labeled 2-azidoadenosine 5"-triphosphate (2N3ATP) and 8-azidoadenosine 5"-triphosphate (8N3ATP), we have identified a site on human interferon alpha2 (IFN-alpha2) that binds adenine nucleotides. 8-azidoadenosine 5'-triphosphate interferon alpha 2 Homo sapiens
3 Using 32P-labeled 2-azidoadenosine 5"-triphosphate (2N3ATP) and 8-azidoadenosine 5"-triphosphate (8N3ATP), we have identified a site on human interferon alpha2 (IFN-alpha2) that binds adenine nucleotides. 8-azidoadenosine 5'-triphosphate interferon alpha 2 Homo sapiens
4 Using 32P-labeled 2-azidoadenosine 5"-triphosphate (2N3ATP) and 8-azidoadenosine 5"-triphosphate (8N3ATP), we have identified a site on human interferon alpha2 (IFN-alpha2) that binds adenine nucleotides. 8-azidoadenosine 5'-triphosphate interferon alpha 2 Homo sapiens
5 Half-maximal saturation of IFN-alpha2 was observed at 10 microM 2N3ATP or 35 microM 8N3ATP. 8-azidoadenosine 5'-triphosphate interferon alpha 2 Homo sapiens
6 Immobilized-Al3+ affinity chromatography and HPLC were used to purify the modified peptides from IFN-alpha2 that had been photolabeled with 8N3ATP and digested with trypsin or chymotrypsin. 8-azidoadenosine 5'-triphosphate interferon alpha 2 Homo sapiens