Title : Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression.

Pub. Date : 1997 Aug 15

PMID : 9269991






6 Functional Relationships(s)
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1 Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Tetracycline tumor necrosis factor Homo sapiens
2 We have recently modified a tetracycline (Tc) repressor/operator-based mammalian gene expression system and have generated a Tc-responsive recombinant adenovirus vector, AdVtTA.TNF-alpha. Tetracycline tumor necrosis factor Homo sapiens
3 We have recently modified a tetracycline (Tc) repressor/operator-based mammalian gene expression system and have generated a Tc-responsive recombinant adenovirus vector, AdVtTA.TNF-alpha. Tetracycline tumor necrosis factor Homo sapiens
4 We have recently modified a tetracycline (Tc) repressor/operator-based mammalian gene expression system and have generated a Tc-responsive recombinant adenovirus vector, AdVtTA.TNF-alpha. Tetracycline tumor necrosis factor Homo sapiens
5 A variety of human tumor cells and T lymphocytes transduced by AdVtTA.TNF-alpha secreted high-titer (5,000-100,000 pg/10(6) cells/24 h) and biologically active TNF-alpha in the absence of Tc. Tetracycline tumor necrosis factor Homo sapiens
6 Expression of TNF-alpha in the transduced cells was nondetectable when the culture medium contained as little as 0.1 microg/ml of Tc. Tetracycline tumor necrosis factor Homo sapiens