Pub. Date : 1997 Mar
PMID : 9056490
1 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | With repeated cycles of freezing and thawing as a first step, the purification of the recombinant ch-DHFR to homogeneity requires only one further step, gel filtration on a Sephadex G-75 column with 85-90% enzyme recovery, two to three times higher than that obtained with the commonly used affinity chromatography on a methotrexate-Sepharose column. | Sepharose | dihydrofolate reductase | Escherichia coli |