Title : Characterization of calcium-dependent forms of protein kinase C in adult rat ventricular myocytes.

Pub. Date : 1997 Jan

PMID : 9046017






3 Functional Relationships(s)
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1 PKC activity was measured in cytosolic and particulate fractions prepared from control myocytes and those treated with either phorbol ester (phorbol 12-myristate 13-acetate, PMA) or a permeant synthetic diacylglycerol analog (1-oleoyl-2-acetylglycerol, OAG) in the absence or presence of an inhibitor of diacylglycerol kinase activity, compound R59022. Tetradecanoylphorbol Acetate protein kinase C, alpha Rattus norvegicus
2 PKC activity was measured in cytosolic and particulate fractions prepared from control myocytes and those treated with either phorbol ester (phorbol 12-myristate 13-acetate, PMA) or a permeant synthetic diacylglycerol analog (1-oleoyl-2-acetylglycerol, OAG) in the absence or presence of an inhibitor of diacylglycerol kinase activity, compound R59022. Tetradecanoylphorbol Acetate protein kinase C, alpha Rattus norvegicus
3 Incubation of the myocytes with PMA, but not OAG, resulted in translocation of PKC from the cytosolic to the particulate fraction. Tetradecanoylphorbol Acetate protein kinase C, alpha Rattus norvegicus