Title : Osmoregulation and protein expression in a pbs2delta mutant of Saccharomyces cerevisiae during adaptation to hypersaline stress.

Pub. Date : 1997 Feb 17

PMID : 9042961






2 Functional Relationships(s)
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1 We deleted the PBS2 gene encoding the MAP kinase activator of the osmosignaling HOG pathway in Saccharomyces cerevisiae and examined the effects on the kinetics of the osmoregulatory glycerol response and protein induction during adaptation to 0.7 M NaCl. Glycerol mitogen-activated protein kinase kinase PBS2 Saccharomyces cerevisiae S288C
2 Northern analysis of the salt induced transcription of GPD1 and GPP2, encoding the cytosolic glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase involved in the osmostress induced glycerol production, demonstrated an about 20-fold PBS2-dependent transient activation, in agreement with the previously reported transient nature of the signal transduced by the HOG pathway. Glycerol mitogen-activated protein kinase kinase PBS2 Saccharomyces cerevisiae S288C