Title : Induction of apoptosis in human lung cancer cells after wild-type p53 activation by methoxyestradiol.

Pub. Date : 1997 Jan 23

PMID : 9018125






7 Functional Relationships(s)
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1 2-Methoxyestradiol (2-MeOE2) treatment caused significant growth inhibition of H460 and A549 human lung cancer cell lines which contain wild-type p53. 2-Methoxyestradiol tumor protein p53 Homo sapiens
2 2-Methoxyestradiol (2-MeOE2) treatment caused significant growth inhibition of H460 and A549 human lung cancer cell lines which contain wild-type p53. 2-Methoxyestradiol tumor protein p53 Homo sapiens
3 Western blot analysis indicated that 2-MeOE2 treatment resulted in an eightfold increase in the endogenous wild-type p53 protein, while the level of the mutant p53 protein remained unchanged. 2-Methoxyestradiol tumor protein p53 Homo sapiens
4 TdT staining indicated that following 2-MeOE2-mediated increases in wildtype p53 protein, cells bypass the G1-S checkpoint of the cell cycle with 30 to 40% undergoing apoptosis. 2-Methoxyestradiol tumor protein p53 Homo sapiens
5 Introduction of anti-sense wt-p53 into wt-p53 cells abrogated the 2-MeOE2 effect. 2-Methoxyestradiol tumor protein p53 Homo sapiens
6 Introduction of anti-sense wt-p53 into wt-p53 cells abrogated the 2-MeOE2 effect. 2-Methoxyestradiol tumor protein p53 Homo sapiens
7 A significant portion of lung cancer retains the wild-type p53 gene therefore, 2-MeOE2 may have therapeutic application. 2-Methoxyestradiol tumor protein p53 Homo sapiens