Pub. Date : 1996 Nov 1
PMID : 8910295
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Endothelial nitric oxide synthase is regulated by tyrosine phosphorylation and interacts with caveolin-1. | Tyrosine | nitric oxide synthase 3 | Bos taurus |
| 2 | Here, we demonstrate that eNOS is tyrosine-phosphorylated in bovine aortic endothelial cells (BAEC) using 32P metabolic labeling followed by phosphoamino acid analysis and by phosphotyrosine specific Western blotting. | Tyrosine | nitric oxide synthase 3 | Bos taurus |
| 3 | Treatment of BAEC with hydrogen peroxide and the protein tyrosine phosphatase inhibitor, sodium orthovanadate, increases eNOS tyrosine phosphorylation. | Tyrosine | nitric oxide synthase 3 | Bos taurus |
| 4 | Because eNOS is localized in plasmalemma caveolae, we examined if tyrosine phosphorylated eNOS interacts with caveolin-1, the coat protein of caveolae. | Tyrosine | nitric oxide synthase 3 | Bos taurus |
| 5 | Conversely, immunoprecipitation of caveolin-1 resulted in the co-precipitation of tyrosine-phosphorylated eNOS. | Tyrosine | nitric oxide synthase 3 | Bos taurus |
| 6 | Thus, tyrosine phosphorylation is a novel regulatory mechanism for eNOS and caveolin-1 is the first eNOS-associated protein. | Tyrosine | nitric oxide synthase 3 | Bos taurus |
| 7 | Collectively, these observations provide a novel regulatory mechanism for eNOS and suggest that tyrosine phosphorylation may influence its activity, subcellular trafficking, and interaction with other caveolin-interacting proteins in caveolae. | Tyrosine | nitric oxide synthase 3 | Bos taurus |