Title : Inadequacy of high K+/nigericin for calibrating BCECF. I. Estimating steady-state intracellular pH.

Pub. Date : 1996 Oct

PMID : 8897819






4 Functional Relationships(s)
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1 This discrepancy became small if pHi had really been approximately 0.2 lower than what was estimated using nigericin-calibrated pHi values. Nigericin glucose-6-phosphate isomerase Rattus norvegicus
2 This discrepancy became small if pHi had really been approximately 0.2 lower than what was estimated using nigericin-calibrated pHi values. Nigericin glucose-6-phosphate isomerase Rattus norvegicus
3 If the true pHi values were approximately 0.2-0.4 lower than our nigericin-calibrated values, then the sum of beta int and beta CO2 equals beta tot. Nigericin glucose-6-phosphate isomerase Rattus norvegicus
4 Finally, one potential source for these discrepancies is described: selecting an inappropriate external K+ to use with nigericin can produce systematic errors in pHi of approximately 0.1. Nigericin glucose-6-phosphate isomerase Rattus norvegicus