Title : Conformational transition of insulin induced by n-alkyltrimethylammonium bromides in aqueous solution.

Pub. Date : 1996 Feb

PMID : 8852753






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1 A surfactant-induced conformational transition of bovine insulin has been detected by difference spectroscopy for a homologous series of n-alkytrimethylammonium bromides, chain length C10-C16 at pH 10.0, 25 degrees C. The transition was followed as a function of surfactant concentration by absorbance measurements at 275 nm and the data were analysed to obtain the Gibbs energy of the transition in water (delta Gw degree) and in a hydrophobic environment (delta Ghc degree) for saturated protein-surfactant complexes. Water insulin Bos taurus