Title : High frequency (139.5 GHz) electron paramagnetic resonance characterization of Mn(II)-H2(17)O interactions in GDP and GTP forms of p21 ras.

Pub. Date : 1996 Sep 17

PMID : 8810926






4 Functional Relationships(s)
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1 X-ray crystallographic studies of p21 suggest that coordination between threonine-35 and the divalent metal ion plays an important role in these conformational changes. Threonine H3 histone pseudogene 16 Homo sapiens
2 Recent ESEEM studies of p21 in solution, however, place threonine-35 more distant from the metal and were interpreted as weak or indirect coordination of this residue. Threonine H3 histone pseudogene 16 Homo sapiens
3 We report high frequency (139.5 GHz) EPR spectroscopy of p21.Mn(II) complexes of two guanine nucleotides that probes the link between threonine-35 and the divalent metal ion. Threonine H3 histone pseudogene 16 Homo sapiens
4 This result rules out indirect coordination of threonine-35 in the solution structure of p21.Mn(II).GMPPNP, and implicates direct, weak coordination of this residue as suggested by Halkides et al. Threonine H3 histone pseudogene 16 Homo sapiens