Title : Further evidence for binding of three single-stranded DNA molecules by the RecA filament.

Pub. Date : 1995 May

PMID : 8586638






4 Functional Relationships(s)
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1 The ability to accommodate three single-strands of DNA by each filamentous RecA-DNA complex, in the presence of the non-hydrolysable ATP analog, adenosine 5"-O-3-thiotriphosphate (ATP gamma S), was demonstrated by monitoring the fast renaturation that occurs between the complementary homopolymers, poly(dA) and poly(dT), upon sodium dodecyl sulfate (SDS)-induced dissociation of RecA. Thymidine RAD51 recombinase Homo sapiens
2 The ability to accommodate three single-strands of DNA by each filamentous RecA-DNA complex, in the presence of the non-hydrolysable ATP analog, adenosine 5"-O-3-thiotriphosphate (ATP gamma S), was demonstrated by monitoring the fast renaturation that occurs between the complementary homopolymers, poly(dA) and poly(dT), upon sodium dodecyl sulfate (SDS)-induced dissociation of RecA. Thymidine RAD51 recombinase Homo sapiens
3 The reaction was fast and complete when SDS was added to a mixture of RecA having three nucleobases per subunit each of poly(dA) and poly(dT), while the reaction was slower and incomplete in the absence of RecA. Thymidine RAD51 recombinase Homo sapiens
4 Similar fast renaturation was observed upon the addition of SDS to a mixture of RecA with three bases each of poly(dA), poly(dT), and a non-complementary DNA, poly(dC), even when one of the complementary DNAs was added last as the third strand. Thymidine RAD51 recombinase Homo sapiens