Title : Efficient replication of polyomavirus DNA in a cell-free system supplemented with Escherichia coli single-stranded DNA binding protein, which exhibits species-specificity in the requirement for DNA polymerase alpha-primase.

Pub. Date : 1995 Aug

PMID : 8543582






1 Functional Relationships(s)
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1 By supplementing with E. coli SSB, we were able to reduce the amount of cell extracts in the reaction mixture, and to lower the concentrations of creatine phosphate and Tris, rendering this system more resistant to salts than the conventional PyV DNA replication system. Phosphocreatine single-stranded DNA-binding protein Escherichia coli