Pub. Date : 1993 Mar
PMID : 8486612
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | In order to characterize the intracellular processing event of lysosomal cathepsin B, the proenzyme was purified from the rat liver microsomal contents using a Con A-Sepharose column, a Sepharose-Gly-Phe-GlySc column, and an anti-cathepsin B IgG column. | Sepharose | cathepsin B | Rattus norvegicus |
2 | In order to characterize the intracellular processing event of lysosomal cathepsin B, the proenzyme was purified from the rat liver microsomal contents using a Con A-Sepharose column, a Sepharose-Gly-Phe-GlySc column, and an anti-cathepsin B IgG column. | Sepharose | cathepsin B | Rattus norvegicus |
3 | When the purified proenzyme was incubated with the cathepsin B-free tritosomal contents, prepared by treatment of the tritosomal contents with anti-cathepsin B IgG Sepharose, at pH 3.0, 30 degrees C, a remarkable increase of enzymatic activity was observed. | Sepharose | cathepsin B | Rattus norvegicus |