Title : Intracellular pH regulation in single cultured astrocytes from rat forebrain.

Pub. Date : 1993 Aug

PMID : 8406681






5 Functional Relationships(s)
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1 Switching to a medium buffered to pH 7.40 with 5% CO2 and 25 mM HCO3(-) caused the steady-state pHi to increase by an average of 0.35, suggesting the presence of a HCO3(-) -dependent acid-extrusion mechanism. Bicarbonates glucose-6-phosphate isomerase Rattus norvegicus
2 Switching to a medium buffered to pH 7.40 with 5% CO2 and 25 mM HCO3(-) caused the steady-state pHi to increase by an average of 0.35, suggesting the presence of a HCO3(-) -dependent acid-extrusion mechanism. Bicarbonates glucose-6-phosphate isomerase Rattus norvegicus
3 In experiments in which astrocytes were exposed to nominally HCO3(-)-free (HEPES-buffered) solutions, the application and withdrawal of 20 mM extracellular NH4+ caused pHi to fall to a value substantially below the initial one. Bicarbonates glucose-6-phosphate isomerase Rattus norvegicus
4 Returning the Na+ caused pHi to increase rapidly, indicating the presence of an Na(+)-dependent/HCO3(-)-dependent acid-extrusion mechanism; the final pHi after returning Na+ was approximately 0.08 higher than the initial value. Bicarbonates glucose-6-phosphate isomerase Rattus norvegicus
5 Returning the Na+ caused pHi to increase rapidly, indicating the presence of an Na(+)-dependent/HCO3(-)-dependent acid-extrusion mechanism; the final pHi after returning Na+ was approximately 0.08 higher than the initial value. Bicarbonates glucose-6-phosphate isomerase Rattus norvegicus