Title : Nucleotide binding and GTP hydrolysis by the 21-kDa product of the c-H-ras gene as monitored by proton-NMR spectroscopy.

Pub. Date : 1993 Apr 15

PMID : 8386636






3 Functional Relationships(s)
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1 Proton-NMR signals in the downfield region (below approximately 10 ppm) have been shown to provide a useful spectroscopic window to monitor the binding of guanine nucleotides to the active site of GTP/GDP-binding proteins via H-bonds, as specified here by the 21-kDa product of the c-H-ras gene (p21). Guanosine Triphosphate H3 histone pseudogene 16 Homo sapiens
2 The time course of the intensity change of certain peaks upon addition of GTP to nucleotide-free p21 corresponds to the GTP hydrolysis rate as determined by HPLC. Guanosine Triphosphate H3 histone pseudogene 16 Homo sapiens
3 The time course of the intensity change of certain peaks upon addition of GTP to nucleotide-free p21 corresponds to the GTP hydrolysis rate as determined by HPLC. Guanosine Triphosphate H3 histone pseudogene 16 Homo sapiens